PRINCIPLE

Iyo membrane yechishandiso chekuyedza yakavharwa neTf antibody padunhu reyedzo uye yembudzi anti tsuro IgG antibody padunhu rekutonga. Lable pad yakaputirwa nefluorescence yakanyorwa anti Tf antibody uye tsuro IgG pamberi. Paunenge uchiyedza sampuli yakanaka, iyo Tf antigen mumuenzaniso inosanganisa nefluorescence yakanyorwa anti Tf antibody, uye gadzira musanganiswa wekudzivirira muviri. Pasi pechiito che immunochromatography, iyo yakaoma inoyerera ichienda kune inonwisa bepa. Kana yakaoma ikapfuura dunhu rekuyedzwa, yakasanganiswa neanti Tf yekuvhara antibody, inoumba nyowani yakaoma.

Kana zvisina kunaka, sampuli haina transferrin kana zvirimo zvakaderera, kuitira kuti immune yakaoma irege kuumbwa. Pachave pasina mutsara mutsvuku munzvimbo yekuona (T). Hazvina mhosva kuti Tf antibody iriko here mumuenzaniso kana kuti kwete, iyo yakasara colloidal yegoridhe-yakanyorwa mbeva inorwisa-munhu IgM monoclonal antibody uye mbudzi inorwisa mbeva IgG antibody yakavharwa munzvimbo yemhando yekudzora (C) mutsara. Ipapo iyo agglutinates inovandudza ruvara munzvimbo yekudzora yemhando, uye mutsara mutsvuku uchaonekwa mu (C). Mutsara mutsvuku ndiwo chiyero chinoonekwa munharaunda yekudzora yemhando (C) yekutonga kana paine masampuli akakwana uye kana iyo chromatography process yakajairika. Inoshandiswawo seyemukati yekudzora chiyero che reagents.

Test Procedure

Ndokumbira uverenge pasuru usati waedza.

1.Bvisa kadhi rekuedza kubva mubhegi refoil, riise patafura yezinga uye nyora.

2.Bvisa chivharo kubva kumuenzaniso wechubhu uye urasire madonhwe maviri ekutanga diluted sample, wedzera madonhwe matatu (anenge 100uL) hapana bubble diluted sample vertically uye zvishoma nezvishoma mumuenzaniso mutsime wekadhi ine dipette yakapiwa. Zvadaro tanga timer.