PRINCIPLE

Iyo membrane yechishandiso chekuyedza yakavharwa nemicroalbumin antigen padunhu rekuyedza uye mbudzi anti tsuro IgG antibody panzvimbo yekutonga. Lable pad yakaputirwa nefluorescence yakanyorwa microalbumin uye tsuro IgG pamberi. Kana musina albumin muweti, colloidal gold-labeled anti-Alb-labeled monoclonal antibody pabepa regoridhe re colloidal inomhanya pamembrane neweti kuenda kumutsara wekuona, uye kusanganisa neAlb-coated antigen ine inooneka. line. Uye ruvara rwemutsara rwakasviba kupfuura ruvara rwemutsara munzvimbo yekutonga (C), iyi mhedzisiro yakaipa. Kana weti iine albumin, vanozokwikwidza neAlb-coated antigen pa membrane kuti isungirire kune mashoma masoja ekudzivirira chirwere pane colloidal goridhe-yakanyorwa anti-Alb-yakanyorwa monoclonal antibody. Sezvo huwandu hwealbumin muweti hunowedzera, kuedza

Muvara wemutsara uchave wakareruka uye wakareruka. Zviri mukati mealbumin muweti zvinogona kuwonekwa semi-quantitatively nekuenzanisa iyo yekuona (T) nzvimbo nenzvimbo yekutonga (C). Iyo yemhando yekudzora nzvimbo (C) uye nzvimbo yekutarisa (R) pakiti ichagara ichionekwa panguva yekuedzwa, uye haina chekuita nekuvapo kwealbumin weti. Nzvimbo yekutonga (C) uye nzvimbo yereferensi (R) mutsara inogona kushandiswa seyemukati yemhando yekudzora index index yekiti.

Test Procedure:

Ndokumbira uverenge bhuku rekushandisa chekushandisa uye pasuru isa usati waedza. Chengetedza sampuli kune tembiricha yekamuri usati washandisa.

1.Bvisa kadhi rekuedza kubva mubhegi refoil. Iise yakatsetseka panzvimbo yakachinjika womaka.

2.Tora weti sampuli nepipette inoraswa, bvisa madonhwe maviri ekutanga emuenzaniso weti. Wedzera madonhwe matatu (angangoita 100uL) eweti isina bubble pakati pegomba remuenzaniso wekadhi rebvunzo rakamira uye tanga kutara nguva.

3.Verenga mhedzisiro mu10-15 maminitsi. Haikodzeri kana inopfuura maminitsi gumi nemashanu.