Pusa Su'esu'ega mo Isoenzyme MB o le Creatine Kinase

fa'amatalaga puupuu:


  • Taimi o su'ega:10-15 minute
  • Taimi aoga:24 masina
  • Sa'o:Sili atu i le 99%
  • Fa'amatalaga:1/25 su'ega/pusa
  • Teuga vevela:2℃-30℃
  • Fa'amatalaga Oloa

    Faailoga o oloa

    Pusa Su'esu'ega mo Isoenzyme MB o Creatine Kinase(su'esu'ega o le immunochromatographic fluorescence)
    Mo na'o le fa'aogaina o su'esu'ega in vitro

    Fa'amolemole faitau ma le toto'a lenei afifi fa'apipi'i a'o le'i fa'aogaina ma mulimulita'i i fa'atonuga. E le mafai ona fa'amaonia le fa'atuatuaina o fa'ai'uga su'esu'e pe a iai ni 'ese'esega mai fa'atonuga o lo'o i totonu o lenei afifi fa'aofi.

    FA'A'OA'OGA
    Su'esu'ega Su'esu'ega mo Isoenzyme MB o Creatine Kinase (fluorescence immunochromatographic assay) o se su'esu'ega immunochromatographic fluorescence mo le su'esu'eina fa'atele o Isoenzyme MB o Creatine Kinase (CK-MB) i le toto o le tagata po'o le plasma, e fa'aaogaina mo su'esu'ega fesoasoani o le AMI (Acute Myocardial). Infarction). O faʻataʻitaʻiga lelei uma e tatau ona faʻamaonia e isi metotia. O lenei su'ega ua fa'amoemoe mo na'o le fa'aoga fa'apolofesa fa'alesoifua maloloina.

    AOTELEGA
    CK-MB maualuga maualuga 4-6 itula ina ua mavae Myocardial infarction tupu, tumutumu i 18-24 itula, ma toe foi i le masani 2-3 aso mulimuli ane. CK-MB fa'amatu'u atu i le ta'amilosaga mulimuli ane nai lo le hemoglobin.CK-MB o le fa'ailoga fa'aleaganu'u o le fa'ama'i tigaina o le myocardial.

    FA'AVAE O LE TULAGA
    O le membrane o le masini su'ega e ufiufi i le anti CK-MB antibody i luga o le vaega o su'ega ma 'oti anti lapiti IgG antibody i luga o le itulagi pulea. O lo'o fa'apipi'iina le pepa fa'ailoga i le fa'amalama e fa'aigoaina le anti CK-MB antibody ma le lapiti IgG muamua. A fa'ata'ita'i fa'ata'ita'iga lelei, o le CK-MB antigen i le fa'ata'ita'iga e tu'u fa'atasi ma le fluorescence e fa'ailoga antibody CK-MB, ma fa'atupu ai le paluga puipuia. I lalo o le gaioiga a le immunochromatography, o le faʻalavelave faʻafefe i le itu o le pepa absorbent, pe a pasia le suʻega o le suʻega, faʻatasi ai ma le anti CK-MB coating antibody, e fausia ai se lavelave fou. Ole maualuga ole CK-MB o lo'o fa'amaopoopo lelei ma fa'ailo fa'amalama, ma o le fa'atonuga ole CK-MB ile fa'ata'ita'iga e mafai ona iloa ile su'ega immunoassay ole fluorescence.

    REAGENTS MA MEA E FAIA

    25T vaega afifi
    Fa'ata'ita'i pepa ta'itasi pepa pepa o lo'o tu'u i ai se mea fa'amago 25T
    Fa'ata'ita'i fa'afefiloi 25T
    Fa'apipi'i afifi 1

    O MEA E MANAOMIA AE LE TUSIA
    Fa'ata'ita'iga koneteina aoina, taimi

    FA'ATA'ITA'I AOPOTI MA TE'UINA
    1. O faʻataʻitaʻiga faʻataʻitaʻiga e mafai ona avea ma serum, heparin anticoagulant plasma poʻo EDTA anticoagulant plasma.

    2.E tusa ai ma metotia masani aoina faʻataʻitaʻiga. Ole sua poo le plasma sample e mafai ona teu ile pusaaisa ile 2-8 ℃ mo le 7 aso ma le cryopreservation i lalo -15°C mo le 6 masina.
    3. Fa'ata'ita'iga uma aloese mai ta'amilosaga fa'aisa-liu.

    TULAFONO O SU'ESU'EGA
    Fa'amolemole faitau le tusi lesona o meafaigāluega ma mea fa'apipi'i afifi a'o le'i faia su'ega.

    1. Tuu ese uma reagents ma samples i le vevela potu.
    2.Tatala le Portable Immune Analyzer (WIZ-A101), ulufale i le faʻamatalaga faʻamatalaga faʻamatalaga e tusa ai ma le faʻaogaina o le meafaifaʻaili, ma ulufale i le atinaʻe faʻamatalaga.
    3. Su'e le fa'ailoga e fa'amaonia ai le mea su'ega.
    4. Aveese le pepa su'ega mai le taga pepa.
    5. Fa'aofi le pepa su'ega i totonu o le avanoa kata, fa'ata'ita'i le QR code, ma fuafua le mea su'ega.
    5.Add 40μL serum poʻo plasma faʻataʻitaʻiga i faʻataʻitaʻiga faʻafefiloi, ma faʻafefiloi lelei ..
    6. Faʻaopoopo 80μL faʻataʻitaʻiga fofo i faʻataʻitaʻiga lelei o le kata.
    7. Kiliki le "suʻega masani" faʻamau, pe a uma le 15 minute, o le a otometi lava ona iloa e le meafaigaluega le kata suʻega, e mafai ona faitau iʻuga mai le faʻaaliga faʻaaliga o le meafaigaluega, ma faʻamaumau / lolomi iuga o suʻega.
    8. Va'ai ile fa'atonuga a le Portable Immune Analyzer(WIZ-A101).

    TULAGA FA'AMANATU
    CK-MB <5ng/mL

    E fautuaina le falesu'esu'e ta'itasi e fa'atutu lana lava tulaga masani e fai ma sui o le aofa'i o tagata ma'i.

    I'UGA O SU'ESU'EGA MA FA'AU'UGA
    .O faʻamatalaga o loʻo i luga o le taunuʻuga lea o le suʻega CK-MB reagent, ma e fautuaina e tatau i fale suʻesuʻe taʻitasi ona faʻatuina se faʻasologa o tau suʻesuʻega CK-MB talafeagai mo le faitau aofaʻi o lenei itulagi. O fa'ai'uga o lo'o i luga e mo na'o fa'amatalaga.

    .O taunuʻuga o lenei metotia e naʻo le faʻaogaina o faʻamaufaʻailoga ua faʻatuina i lenei metotia, ma e leai se faʻatusatusa tuusaʻo ma isi metotia.
    .O isi mea e mafai foi ona mafua ai mea sese i taunuuga o suʻesuʻega, e aofia ai mafuaʻaga faʻapitoa, mea sese faʻagaioiga ma isi faʻataʻitaʻiga.

    TEUINA MA LE MALAMAU
    1.O le pusa o le 18 masina faʻaola-ola mai le aso na gaosia ai. Taofi pusa e le'i fa'aaogaina ile 2-30°C. AUA LE FA'AISA. Aua le fa'aaogaina i tua atu o le aso e muta ai.

    2. Aua le tatalaina le taga faʻamaufaʻailoga seia e sauni e fai se suʻega, ma o le suʻega faʻaoga tasi e fautuaina e faʻaaoga i lalo o le siosiomaga manaʻomia (vevela 2-35 ℃, susu 40-90%) i totonu ole 60 mins vave. pe a mafai.
    3. Fa'ata'ita'iga fa'afefiloi e fa'aaoga vave pe a uma ona tatalaina.

    LAPATA’IGA MA FA’AALIGA
    .O le pusa e tatau ona faʻamaufaʻailogaina ma puipuia mai le susu.

    .O fa'ata'ita'iga lelei uma e tatau ona fa'amaonia e isi metotia.
    .O fa'ata'ita'iga uma e tatau ona fa'atatauina e ono fa'aleagaina.
    .AUA LE fa'aogaina le reagent ua uma le aoga.
    .AUA LE fesuia'i reagents i pusa ma eseese lot Nu..
    .'A'E LE toe fa'aogaina kata su'ega ma so'o se mea fa'aogaina.
    .Misoperation, fa'atele pe la'ititi fa'ata'ita'iga e mafai ona ta'ita'iina i'uga fa'aletonu.

    LFA'A'ITA'I
    .E pei o so'o se su'esu'ega e fa'aogaina ai fa'ama'i isumu, o lo'o iai le avanoa mo le fa'alavelaveina e tagata fa'ama'i isu (HAMA) i totonu o le fa'ata'ita'iga. O fa'ata'ita'iga mai tagata mama'i na maua tapenaga o monoclonal antibodies mo su'esu'ega po'o togafitiga e ono iai le HAMA. O ia faʻataʻitaʻiga e mafai ona mafua ai iʻuga lelei sese poʻo iʻuga leaga sese.

    .O lenei suʻega suʻega e naʻo le suʻesuʻeina o falemaʻi, e le tatau ona naʻo le pau lea o le faʻavae mo suʻesuʻega faʻapitoa ma togafitiga, e tatau i tagata maʻi ona tuʻufaʻatasia le iloiloga faʻatasi ma ona faʻamaoniga, talaʻaga faʻafomaʻi, isi suʻesuʻega suʻesuʻe, tali togafitiga, epidemiology ma isi faʻamatalaga .
    .O lenei reagent e naʻo le faʻaaogaina mo suʻega toto ma plasma. Atonu e le maua se taunuuga saʻo pe a faʻaaogaina mo isi faʻataʻitaʻiga e pei ole faua ma urine ma isi.

    UIGA FAATINO

    Linearity 0.5ng/mL i le 80ng/mL va'aiga vavalalata:-15% i le +15%.
    Fa'asologa fa'atasi laina:(r)≥0.9900
    Sa'o Ole fua ole toe fa'aleleia e tatau ona i totonu ole 85% - 115%.
    Toe fai CV≤15%
    Fa'apitoa(E leai se mea i le faʻalavelave faʻalavelave faʻalavelave faʻalavelave i le suʻega)

    Fa'alavelave

    Fa'alavelave fa'alavelave

    sTnI

    1000μg/L

    cTnT

    1000μg/L

    ABP

    1000μg/L

    cTnI

    1000μg/L

    cTnC

    1000μg/L

    sTnT

    1000μg/L

    LO'U

    1000μg/L

    RFAAMATALAGA
    1.Hansen JH,et al.HAMA Fa'alavelave ile Murine Monoclonal Antibody-Based Immunoassays[J].J of Clin Immunoassay,1993,16:294-299.

    2.Levinson SS.O le Natura o Heterophilic Antibodies ma le matafaioi i le Immunoassay Interference[J].J of Clin Immunoassay,1992,15:108-114.

    Ki i fa'ailoga fa'aoga:

     t11-1 In Vitro Diagnostic Medical Device
     tt-2 Tufuga
     tt-71 Teu i le 2-30 ℃
     tt-3 Uma le aoga
     tt-4 Aua le Toe Fa'aaogaina
     tt-5 FAATUATUA
     tt-6 Fa'atalanoa Fa'atonuga mo le Fa'aaogā

    Xiamen Wiz Biotech CO.,LTD
    Tuatusi:3-4 Folafola, NO.16 Fale, Bio-medical Workshop, 2030 Wengjiao West Road, Haicang District, 361026, Xiamen, Saina
    Telefoni: + 86-592-6808278
    Fax:+86-592-6808279


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