Pusa Su'esu'ega mo Calprotectin (Fluorescence Immunochromatographic Su'ega)

fa'amatalaga puupuu:


  • Taimi o su'ega:10-15 minute
  • Taimi aoga:24 masina
  • Sa'o:Sili atu i le 99%
  • Fa'amatalaga:1/25 su'ega/pusa
  • Teuga vevela:2℃-30℃
  • Fa'amatalaga Oloa

    Faailoga o oloa

    Pusa Su'esu'ega moCalprotectin(Fluorescence Immunochromatographic Assay)
    Mo na'o le fa'aogaina o su'esu'ega in vitro

    Fa'amolemole faitau ma le toto'a lenei afifi fa'apipi'i a'o le'i fa'aogaina ma mulimulita'i i fa'atonuga. E le mafai ona fa'amaonia le fa'atuatuaina o fa'ai'uga su'esu'e pe a iai ni 'ese'esega mai fa'atonuga o lo'o i totonu o lenei afifi fa'aofi.

    FA'A'OA'OGA
    Pusa Su'esu'ega moCalprotectin(Cal) e talafeagai mo le fuaina o le faeces tagata Cal e ala i le fluorescence immunochromatographic assay, lea e taua fesoasoani taua diagnostic mo ma'i inflammatory bowel.All lelei faataitaiga e tatau ona faamaonia e isi methodologies. O lenei su'ega ua fa'amoemoe mo na'o le fa'aoga fa'apolofesa fa'alesoifua maloloina.

    AOTELEGA
    Cal o se heterodimer, lea e aofia ai le MRP 8 ma le MRP 14[1]. O lo'o i ai i neutrophils cytoplasm ma fa'aalia i luga o membranes cell mononuclear. O le kal o se polotini vaega ma'ai, o lo'o i ai se vaega mautu pe a ma le tasi le vaiaso i fe'au o le tagata, e fa'amautu e avea ma fa'ailoga fa'ama'i inflammatory bowel.[2-3]. O le pusa o se suʻega faʻataʻitaʻi faigofie, faʻataʻitaʻiga e iloa ai Cal i feʻau o tagata, e maualuga lona lagona maʻaleʻale ma malosi faʻapitoa. O le su'ega e fa'avae i luga ole tulaga maualuga ole fa'aogaina o le sanuisi fa'ama'i fa'alua ma le fa'aogaina ole immunochromatographic su'esu'ega tekinolosi, lea e mafai ona maua ai se fa'ai'uga i totonu ole 15 minute.

    FA'AVAE O LE TULAGA
    O lo'o iai le anti-Cal coating antibody i le vaega o su'ega, lea e fa'amau i le chromatography membrane i luma atu. O lo'o fa'apipi'iina le pepa fa'aigoa i le fluorescence ua fa'ailoga anti-Cal antibody muamua. A fa'ata'ita'i fa'ata'ita'iga lelei, o le Cal i fa'ata'ita'iga e mafai ona fa'afefiloi ma le fluorescence fa'ailoga anti-Cal antibody, ma fa'atupu ai le paluga puipuia. A'o fa'ataga le fa'afefiloi e femalaga'i i luga o le fasi pepa su'ega, o le Cal conjugate lavelave e pu'eina e le anti-Cal coating antibody i luga o le membrane ma fa'atupu fa'alavelave. Ole malosi ole fluorescence e fetaui lelei ma le Cal content. Ole Cal ile fa'ata'ita'iga e mafai ona iloa ile fluorescence immunoassay analyzer.

    REAGENTS MA MEA E FAIA

    25T vaega afifi
    Fa'ata'ita'i pepa ta'itasi pepa pepa o lo'o tu'u i ai se mea fa'amago 25T
    Fa'ata'ita'i fa'afefiloi 25T
    Fa'apipi'i afifi 1

    O MEA E MANAOMIA AE LE TUSIA
    Fa'ata'ita'iga koneteina aoina, taimi

    FA'ATA'ITA'I AOPOTI MA TE'UINA
    1. Fa'aaogā se atigipusa mama tu'u'eseina e aoina ai fa'ata'ita'i fe'ai fou, ma fa'ata'ita'i vave. Afai e le mafai ona faʻataʻitaʻi vave, faʻamolemole teu i le 2-8°C mo le 3 aso poʻo lalo ifo -15°C mo le 6 masina.

    2. Ave ese le la'au fa'ata'ita'i, fa'aofi i totonu o le fa'ata'ita'i fa'ata'ita'i, toe fai le gaioiga fa'a 3 taimi, ave vaega 'ese'ese ole fa'ata'ita'iga fa'ata'ita'i i taimi ta'itasi, ona toe tu'u lea i tua le la'au fa'ata'ita'i, fa'amalo fa'amalo ma lulu lelei, Po'o le fa'aogaina o le la'au fa'ata'ita'i na pikiina. e tusa ma le 50mg faeces sample, ma tu'u i totonu o le faeces sample tube o lo'o i ai fa'ata'ita'iga fa'afefiloi, ma fa'amau fa'amau.
    3. Fa'aaogā fa'ata'ita'iga paipa lafoa'i ave fa'ata'ita'i feces mai le ma'i manava tatā, fa'aopoopo 3 mataua (pe tusa ma le 100µL) i le fa'asu'amea fa'ameamea ma lulu lelei.

    Fa'amatalaga:
    1. Aloese mai ta'amilosaga fa'a'aisa.

    2.Thaw faʻataʻitaʻiga ile vevela potu aʻo leʻi faʻaaogaina.

    TULAFONO O SU'ESU'EGA

    Fa'amolemole faitau le tusi lesona o meafaigāluega ma mea fa'apipi'i afifi a'o le'i faia su'ega.
    1. Tuu ese uma reagents ma samples i le vevela potu.
    2.Tatala le Portable Immune Analyzer (WIZ-A101), ulufale i le faʻamatalaga faʻamatalaga faʻamatalaga e tusa ai ma le faʻaogaina o le meafaifaʻaili, ma ulufale i le atinaʻe faʻamatalaga.
    3. Su'e le fa'ailoga e fa'amaonia ai le mea su'ega.
    4. Aveese le pepa su'ega mai le taga pepa.
    5. Fa'aofi le pepa su'ega i totonu o le avanoa kata, fa'ata'ita'i le QR code, ma fuafua le mea su'ega.
    6. Aveese le pulou mai le paipa faʻataʻitaʻiga ma lafoaʻi muamua lua mataua faʻafefiloi faʻataʻitaʻiga, faʻaopopo 3 mataua (tusa ma le 100uL) leai se faʻataʻitaʻiga faʻafefeteina faʻataʻitaʻi saʻo ma faʻagesegese i totonu o le faʻataʻitaʻiga vaieli o le kata ma tuʻuina atu dispette.
    7. Kiliki le "suʻega masani" faʻamau, pe a uma le 15 minute, o le a otometi lava ona iloa e le meafaigaluega le kata suʻega, e mafai ona faitau iʻuga mai le faʻaaliga faʻaaliga o le meafaigaluega, ma faʻamaumau / lolomi iuga o suʻega.
    8. Va'ai ile fa'atonuga a le Portable Immune Analyzer(WIZ-A101).

    TULAGA FA'AMANATU
    Cal <60μg/g

    E fautuaina le falesu'esu'e ta'itasi e fa'atutu lana lava tulaga masani e fai ma sui o le aofa'i o tagata ma'i.

    I'UGA O SU'ESU'EGA MA FA'AU'UGA
    1.O le Cal i le faʻataʻitaʻiga e sili atu i le 60μg / g, ma e tatau ona faʻaumatia le suiga o le tulaga o le physiological. O fa'ai'uga e moni e le masani ma e tatau ona fa'amauina i fa'ailoga fa'afoma'i.

    2. O taunuʻuga o lenei metotia e naʻo le faʻaogaina o faʻamaumauga o loʻo faʻatuina i lenei metotia, ma e leai se faʻatusatusa tuusaʻo ma isi metotia.
    3.O isi mea e mafai foi ona mafua ai mea sese i taunuuga o le suʻesuʻeina, e aofia ai mafuaʻaga faʻapitoa, faʻaoga sese ma isi mea faʻataʻitaʻi.

    TEUINA MA LE MALAMAU
    1.O le pusa o le 18 masina faʻaola-ola mai le aso na gaosia ai. Taofi pusa e le'i fa'aaogaina ile 2-30°C. AUA LE FA'AISA. Aua le fa'aaogaina i tua atu o le aso e muta ai.

    2. Aua le tatalaina le taga faʻamaufaʻailoga seia e sauni e fai se suʻega, ma o le suʻega faʻaoga tasi e fautuaina e faʻaaoga i lalo o le siosiomaga manaʻomia (vevela 2-35 ℃, susu 40-90%) i totonu ole 60 mins vave. pe a mafai.
    3. Fa'ata'ita'iga fa'afefiloi e fa'aaoga vave pe a uma ona tatalaina.

    LAPATA’IGA MA FA’AALIGA
    .O le pusa e tatau ona faʻamaufaʻailogaina ma puipuia mai le susu.

    .O fa'ata'ita'iga lelei uma e tatau ona fa'amaonia e isi metotia.
    .O fa'ata'ita'iga uma e tatau ona fa'atatauina e ono fa'aleagaina.
    .AUA LE fa'aogaina le reagent ua uma le aoga.
    .AUA LE fesuia'i reagents i pusa ma eseese lot Nu..
    .'A'E LE toe fa'aogaina kata su'ega ma so'o se mea fa'aogaina.
    .Misoperation, fa'atele pe la'ititi fa'ata'ita'iga e mafai ona ta'ita'iina i'uga fa'aletonu.

    TAULAGA
    .E pei o so'o se su'esu'ega e fa'aogaina ai fa'ama'i isumu, o lo'o iai le avanoa mo le fa'alavelaveina e tagata fa'ama'i isu (HAMA) i totonu o le fa'ata'ita'iga. O fa'ata'ita'iga mai tagata mama'i na maua tapenaga o monoclonal antibodies mo su'esu'ega po'o togafitiga e ono iai le HAMA. O ia faʻataʻitaʻiga e mafai ona mafua ai iʻuga lelei sese poʻo iʻuga leaga sese.

    .O lenei suʻega suʻega e naʻo le suʻesuʻeina o falemaʻi, e le tatau ona naʻo le pau lea o le faʻavae mo suʻesuʻega faʻapitoa ma togafitiga, e tatau i tagata maʻi ona tuʻufaʻatasia le iloiloga faʻatasi ma ona faʻamaoniga, talaʻaga faʻafomaʻi, isi suʻesuʻega suʻesuʻe, tali togafitiga, epidemiology ma isi faʻamatalaga .
    .O lenei reagent e faʻaaogaina mo suʻega fecal. Atonu e le maua se taunuuga saʻo pe a faʻaaogaina mo isi faʻataʻitaʻiga e pei ole faua ma urine ma isi.

    UIGA FAATINO

    Linearity 10μg/g i le 2400μg/g va'aiga vavalalata:-15% i le +15%.
    Fa'asologa fa'atasi laina:(r)≥0.9900
    Sa'o Ole fua ole toe fa'aleleia e tatau ona i totonu ole 85% - 115%.
    Toe fai CV≤15%
    Fa'apitoa(E leai se mea i le fa'alavelave fa'ata'ita'i na fa'alavelave ile su'ega) Fa'alavelave Fa'alavelave fa'alavelave
    Hemoglobin 200μg/mL
    transferrin 100μg/mL
    Horse radish peroxidase 2000μg/mL

    FAAMATALAGA
    1.Li,G.&Y.L.Li.O le sootaga i le va o le calcium ma le maʻi faʻamaʻi[J].The Journal of Practical Medicine,2007,23(15)

    2.Han,W.,Xu,JM,et al.O le suʻesuʻega faʻapitoa o calamine ma lactoferrin i tagata mamaʻi ma le ulcerative colitis [J]. Chinese Journal of Gastroenterology, 2010,15(4)
    3.Wang,ZH,Guo,HB,et al.O le suʻesuʻega i le va o fecal calamine ma faʻamaʻi inflammatory bowel [J]. Saienisi Tekonolosi ma Inisinia,2010-03,10(8)

    Ki i fa'ailoga fa'aoga:

     t11-1 In Vitro Diagnostic Medical Device
     tt-2 Tufuga
     tt-71 Teu i le 2-30 ℃
     tt-3 Uma le aoga
     tt-4 Aua le Toe Fa'aaogaina
     tt-5 FAATUATUA
     tt-6 Fa'atalanoa Fa'atonuga mo le Fa'aaogā

    Xiamen Wiz Biotech CO.,LTD
    Tuatusi:3-4 Folafola, NO.16 Fale, Bio-medical Workshop, 2030 Wengjiao West Road, Haicang District, 361026, Xiamen, Saina
    Telefoni: + 86-592-6808278
    Fax:+86-592-6808279


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