Tuzitangira guha abaguzi bacu b'icyubahiro dukoresheje serivisi zishishikaye cyane kubiciro byavuzweUbushinwa Lh IkizaminiStrip Ashyushye Igurishwa Lh Ovulation Ikizamini Hagati, Turakora tubikuye ku mutima gutanga serivisi nziza kubakiriya bose nabacuruzi.
Tuzitangira guha abaguzi bacu b'icyubahiro dukoresheje serivisi zitekerejwe cyaneUbushinwa Lh Ikizamini, Ibizamini, Isosiyete yacu izubahiriza "Ubwiza bwa mbere ,, gutunganirwa ubuziraherezo, bushingiye ku bantu, guhanga udushya" filozofiya y'ubucuruzi. Akazi gakomeye kugirango ukomeze gutera imbere, guhanga udushya mu nganda, kora ibishoboka byose kugirango umushinga wo mucyiciro cya mbere. Turagerageza uko dushoboye kugira ngo twubake uburyo bwo kuyobora siyanse, twige ubumenyi bwinshi bwumwuga, dutezimbere ibikoresho byumusaruro bigezweho hamwe nuburyo bwo kubyaza umusaruro, gukora ibicuruzwa byambere byita ibicuruzwa byiza nibisubizo, igiciro cyiza, serivisi nziza, gutanga vuba, gutanga urema agaciro gashya.
Igikoresho cyo gusuzuma kuri Alpha-fetoprotein(fluorescence immunochromatographic assay)
Kuri vitro kwisuzumisha koresha gusa
Nyamuneka soma iyi paki shyiramo witonze mbere yo gukoresha kandi ukurikize neza amabwiriza. Ibisubizo byizewe ntibishobora kwemezwa niba hari gutandukana kwamabwiriza muriyi paki yinjizamo.

GUKORESHA

Igikoresho cyo gusuzuma indwara ya Alpha-fetoprotein (fluorescence immunochromatographic assay) ni fluorescence immunochromatographic assay yo kumenya umubare wa Alpha-fetoproteine ​​(AFP) muri serumu yumuntu cyangwa plasma, ikoreshwa cyane mugupima indwara zifasha, kuvura no kuvura kanseri yibanze ya kanseri y'umwijima. Icyitegererezo cyiza cyose kigomba kwemezwa nubundi buryo. Iki kizamini kigenewe ubuvuzi bwumwuga gusa.

INCAMAKE

Alpha-fetoprotein (AFP) ni kimwe mu bimenyetso bikoreshwa cyane mu bibyimba.Ni glycoproteine ​​ifite uburemere bwa molekile ingana na 70.000 hamwe nisukari ya 4% .Byakozwe cyane cyane n'umwijima w'inda, bigakurikirwa n'umuhondo wa sac. Uruhinja rwatangiye guhuza Ibyumweru 6, bigera ku mpinga yibyumweru 12 kugeza kuri 15, serumu yibanze kuri 1 kugeza kuri 3 g / L, hamwe namaraso yumutima mugihe cyo kuvuka 10 mg / 100 mg; 90 kugeza 500 ng / mL hagati; Ubusanzwe serumu yumuntu AFP iri hagati ya 2 na 8 ng / mL, ariko indwara nyinshi, cyane cyane hepatite, zigira ingaruka ku gaciro ka AFP.

IHame RY'UBURYO

Ibice by'igikoresho cyo kwipimisha bisizwe na antibody anti-AFP mukarere k'ibizamini hamwe na antibody y'ihene irwanya urukwavu IgG mukarere kayobora. Lable pad isizwe na fluorescence yanditseho antibody ya AFP ninkwavu IgG mbere. Iyo ugerageza icyitegererezo cyiza, antigen ya AFP murugero ikomatanya na fluorescence yanditseho antibody ya AFP, hanyuma igakora imvange yumubiri. Mubikorwa bya immunochromatografiya, urujya n'uruza rugana mu cyerekezo cyimpapuro zinjira, iyo complexe yatsinze akarere k’ibizamini, ihujwe na anti-AFP coating antibody, ikora urwego rushya.Urwego rwa AFP rufitanye isano neza n’ikimenyetso cya fluorescence, hamwe n’ubushakashatsi bwa AFP murugero rushobora gutahurwa na fluorescence immunoassay assay.

REAGENTS N'IBIKORWA BYUZUWE

25T ibice：

.Ikarita yipimisha kugiti cyawe ifu yometse kuri desiccant 25T
.Urugero rwimikorere 25T
.Paki yinjizamo 1

IBIKORWA BISABWA ARIKO NTIBITANZWE
Icyitegererezo cyo gukusanya ibikoresho, igihe
GUKORANYA URUGERO N'UBubiko
1.Ingero zapimwe zishobora kuba serumu, heparin anticoagulant plasma cyangwa plasma ya EDTA anticoagulant.
2.Kurikije tekiniki zisanzwe zegeranya icyitegererezo. Icyitegererezo cya serumu cyangwa plasma birashobora kubikwa muri firigo kuri 2-8 ℃ muminsi 7 hamwe no kubika munsi -15 ° C mumezi 6.
3.Icyitegererezo cyose wirinde gukonjesha.

GUKORA UBURYO
Nyamuneka soma igitabo gikoreshwa nigikoresho cyo gushyiramo mbere yo kugerageza.
1. Shira ku ruhande reagent zose hamwe nicyitegererezo kubushyuhe bwicyumba.
2.Fungura Portable Immune Analyser (WIZ-A101), andika ijambo ryibanga rya konte ukurikije uburyo bwo gukora igikoresho, hanyuma winjire muburyo bwo gutahura.
3.Shobora kode ya dentification kugirango wemeze ikintu cyizamini.
4.Kuramo ikarita yikizamini mu mufuka wa file.
5. Shyiramo ikarita yikizamini ahantu h'ikarita, suzuma kode ya QR, hanyuma umenye ikintu cyizamini.
6. Ongeramo 20μL serumu cyangwa plasma icyitegererezo kugirango utange urugero, hanyuma uvange neza ..
7. Ongeraho 80μL igisubizo cyicyitegererezo kugirango utange neza ikarita.
8.Kanda buto ya "ikizamini gisanzwe", nyuma yiminota 15, igikoresho kizahita kimenya ikarita yikizamini, irashobora gusoma ibisubizo bivuye kuri ecran yerekana igikoresho, hanyuma wandike / wandike ibisubizo byikizamini.
9. Reba ku mabwiriza ya Portable Immune Analyser (WIZ-A101).

AGACIRO KATEGANYIJWE

AFP ：＜ 10ng / mL
Birasabwa ko buri laboratoire ishyiraho urwego rwayo rusanzwe ruhagarariye abaturage barwaye.

IBISUBIZO BY'IKIZAMINI NO GUSOBANURA
.Amakuru yavuzwe haruguru ni ibisubizo byikizamini cya AFP reagent, kandi birasabwa ko buri laboratoire igomba gushyiraho indangagaciro zerekana AFP zibereye abaturage muri kano karere. Ibisubizo byavuzwe haruguru nibisobanuro gusa.
.Ibisubizo byubu buryo birakoreshwa gusa murwego rwoherejwe rwashyizweho muri ubu buryo, kandi ntaho bihuriye nubundi buryo.
.Ibindi bintu birashobora kandi gutera amakosa mubisubizo byo gutahura, harimo impamvu za tekiniki, amakosa yimikorere nibindi bintu byintangarugero.

Ububiko N'UBUHAMYA
1.Ibikoresho ni amezi 18 yo kubaho-uhereye igihe byakorewe. Bika ibikoresho bidakoreshwa kuri 2-30 ° C. NTUBUNTU. Ntugakoreshe kurenza itariki izarangiriraho.
2.Ntukingure umufuka ufunze kugeza igihe witeguye gukora ikizamini, kandi ikizamini kimwe cyo gukoreshwa gisabwa gukoreshwa mubisabwa (ubushyuhe 2-35 ℃, ubuhehere 40-90%) muminota 60 byihuse. bishoboka.
3.Urugero rwa diluent rukoreshwa ako kanya nyuma yo gufungura.

UMUBURO N'UBWITONDERWA
.Ibikoresho bigomba gufungwa kandi bikarindwa ubushuhe.
.Ingero zose nziza zemezwa nubundi buryo.
.Ingero zose zifatwa nkibishobora guhumanya.
.Ntugakoreshe reagent yarangiye.
. SI guhinduranya reagent mubikoresho bifite ubufindo butandukanye Oya ..
.NtUGASUBIZE gukoresha amakarita yikizamini nibikoresho byose bikoreshwa.
.Gukoresha nabi, urugero rwinshi cyangwa ruto rushobora kuganisha ku gutandukana.

LKwigana
.Nkuko hamwe nubushakashatsi ubwo aribwo bwose bukoresha antibodi yimbeba, birashoboka ko habaho kwivanga na antibodiyite zabantu barwanya imbeba (HAMA) murugero. Ingero z'abarwayi bahawe imyiteguro ya antibodiyite ya monoclonal yo gusuzuma cyangwa kuvura irashobora kuba irimo HAMA. Ingero nkizo zishobora gutera ibinyoma byiza cyangwa ibinyoma bibi.
.Ibisubizo by'ibizamini ni ibyerekeye ivuriro gusa, ntibigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura kwa muganga, imicungire y’amavuriro y’abarwayi igomba kwitabwaho byimazeyo hamwe n’ibimenyetso byayo, amateka y’ubuvuzi, ibindi bizamini bya laboratoire, igisubizo cy’ubuvuzi, epidemiologiya nandi makuru .
.Iyi reagent ikoreshwa gusa mugupima serumu na plasma. Ntishobora kubona ibisubizo nyabyo mugihe ikoreshejwe izindi ngero nka macandwe ninkari nibindi.

IBIKORWA BIKORWA

RINGARUKA
1.Hansen JH, n'abandi.HAMA Kwivanga kwa Murine Monoclonal Antibody ishingiye kuri Immunoassays [J] .J ya Clin Immunoassay, 1993,16: 294-299.
2.Levinson S.

Urufunguzo rwibimenyetso byakoreshejwe:

	Mubikoresho byubuvuzi bya Vitro
	Uruganda
	Ubike kuri 2-30 ℃
	Itariki izarangiriraho
	Ntugakoreshe
	ICYITONDERWA
	Baza Amabwiriza yo Gukoresha

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