IHame

Ibice by'ibikoresho bipimisha bishyizwe hamwe na Groupe A na antenovirus antigen mu karere k'ibizamini hamwe n'ihene irwanya urukwavu IgG antibody ku karere kayobora. Lable pad isizwe na fluorescence yanditseho anti Group A na adenovirus ninkwavu IgG mbere. Iyo ugerageza icyitegererezo cyiza kubitsinda A na adenovirus, Itsinda A na adenovirus murugero bihuza hamwe na fluorescence yanditseho anti Rotavirus Group A na adenovirus, hanyuma igakora imvange yubudahangarwa. Mubikorwa bya immunochromatografiya, urujya n'uruza rwerekezo rwimpapuro zinjira. Iyo complexe yatsinze akarere k'ibizamini, yahujwe na anti-Rotavirus Group A na antibody ya adenovirus, ikora urwego rushya. Niba ari bibi, nta tsinda rya Rotavirus A na antenovirus antigen muri sample, kugirango ingirabuzimafatizo zidashobora gushingwa, ntihazabaho umurongo utukura ahantu hagaragara (T). Hatitawe ku kumenya niba itsinda A rotavirus na adenovirus bihari murugero, imbeba ya latex yanditseho imbeba IgG ni chromatografiya ahantu hagenzurwa ubuziranenge (C) kandi igafatwa na antibody y'ihene irwanya imbeba. Umurongo utukura uzagaragara mugace kagenzura ubuziranenge (C). Umurongo utukura nicyo gisanzwe kigaragara ahantu hagenzurwa ubuziranenge (C) kugirango harebwe niba hari ingero zihagije kandi niba inzira ya chromatografiya ari ibisanzwe. Irakoreshwa kandi nkigenzura ryimbere kuri reagent.

Uburyo bw'ikizamini:

1. Fungura ingofero yicyitegererezo cyo gukusanya. Ntugasuke igisubizo mumacupa.
2. Kuramo inkoni y'icyitegererezo, winjizwe mucyitegererezo cy'umwanda (cyangwa ukoreshe inkoni y'icyitegererezo kugirango uhitemo hafi 50mg y'umwanda), hanyuma usubize inkoni y'icyitegererezo inyuma, usunike neza kandi uzunguze neza, subiramo ibikorwa inshuro 3. Fata igice gitandukanye cyumwanda icyitegererezo buri gihe. Nyuma yo gutoranya, shyira inkoni y'icyitegererezo mu muyoboro wo gukusanya umwanda urimo icyitegererezo cya diluent, hanyuma usunike igitonyanga neza. Niba umwanda wumurwayi urwaye impiswi ari muto, ibyatsi bya pulasitike birashobora gukoreshwa mugupima. Ukoresheje icyitegererezo cya pipette ikuramo fata icyitegererezo cyoroshye cyumurwayi wimpiswi, hanyuma ongeramo ibitonyanga 3 (hafi 100uL) kumuyoboro wa fecal.
3. Shyira neza icyitegererezo hanyuma ukureho umupira hejuru yigitonyanga hanyuma ushire kuruhande.
4. Iyo bibitswe ku bushyuhe buke, ibikoresho bigomba gusubizwa ubushyuhe bwicyumba mbere yo kubikoresha. Kuramo ikarita yikizamini mu mufuka wa file, uyishyire kumeza urwego hanyuma ushireho akamenyetso.
5. Kuraho ingofero muri sample ya sample hanyuma ujugunye ibitonyanga bibiri byambere byongewemo icyitegererezo, ongeramo ibitonyanga 3 (hafi 100uL) ntagituba kivanze cyicyitegererezo gihagaritse kandi gahoro gahoro murugero rwikarita hamwe na disikuru yatanzwe, tangira igihe.
6. Ibisubizo bigomba gusomwa muminota 10-15, kandi ntibyemewe nyuma yiminota 15.