INCAMAKE

Vitamine D ni vitamine kandi ni na hormone ya steroid, cyane cyane harimo VD2 na VD3, imitekerereze yayo isa cyane. Vitamine D3 na D2 bihindurwamo vitamine D ya hydroxyl 25 (harimo vitamine D-25 ya dihydroxyl D3 na D2). 25- (OH) VD mumubiri wumuntu, guhagarara neza, kwibanda cyane. 25- (OH) VD yerekana urugero rwa vitamine D yose, hamwe nubushobozi bwo guhindura vitamine D, bityo 25- (OH) VD ifatwa nkikimenyetso cyiza cyo gusuzuma urwego rwa vitamine D.Igitabo cyo gusuzuma gishingiye immunochromatography kandi irashobora gutanga ibisubizo muminota 15.

IHame RY'UBURYO

Ibice by'igikoresho cyo kwipimisha bisizwe hamwe na conjugate ya BSA na 25- (OH) VD mukarere k'ibizamini hamwe na antibody y'ihene irwanya urukwavu IgG mukarere kayobora. Ikimenyetso cya marike gitwikiriwe na fluorescence ikimenyetso kirwanya antibody 25- (OH) VD hamwe ninkwavu IgG mbere. Iyo ugerageza icyitegererezo, 25- (OH) VD muri sample ikomatanya na fluorescence yanditseho antibody 25- (OH) VD, hanyuma igakora imvange yubudahangarwa. Mubikorwa byubudahangarwa bw'umubiri, urujya n'uruza rugana mu cyerekezo cy'impapuro zinjira, iyo complexe yatsinze akarere k'ibizamini, Ikimenyetso cya fluorescent yubusa kizahuzwa na 25- (OH) VD kuri membrane.Ubushuhe bwa 25- (OH) VD ni ihuriro ribi ryerekana ibimenyetso bya fluorescence, kandi ubunini bwa 25- (OH) VD murugero birashobora gutahurwa na fluorescence immunoassay assay.