GUKORESHA

Igikoresho cyo gusuzuma kuri Calprotectin (cal) ni zahabu ya colloidal immunochromatographic assay yo kugena semiquantitative ya cal kuva mumyanda yabantu, ifite isuzumabumenyi ryingenzi
agaciro k'indwara yo mu mara. Iki kizamini ni reagent. Icyitegererezo cyiza cyose kigomba kuba
byemejwe nubundi buryo. Iki kizamini kigenewe ubuvuzi bwumwuga gusa.
Hagati aho, iki kizamini gikoreshwa kuri IVD, ibikoresho byinyongera ntibikenewe.
INCAMAKE
Cal ni heterodimer, igizwe na MRP 8 na MRP 14. Ibaho muri neutrophile cytoplasm
kandi bigaragarira kuri selile monon nuclear. Cal ni poroteyine ikaze, ifite igihagararo cyiza
icyiciro hafi icyumweru kimwe mumyanda yabantu, yiyemeje kuba ikimenyetso cyindwara yumura.
Igikoresho ni ikizamini cyoroshye, cyerekanwe semiqualitative igaragaza inyana mumyanda yabantu, ifite ubushakashatsi bwinshi
ibyiyumvo kandi byihariye. Ikizamini gishingiye kuri antibodiyite ebyiri zidasanzwe sandwich
Ihame rya reaction na zahabu immunochromatographic assay isesengura tekinike, irashobora gutanga ibisubizo
mu minota 15.
IHame RY'UBURYO
Igice gifite anti cal coating McAb mukarere ka test hamwe na ihene irwanya urukwavu antibody igenzura
karere, kafunzwe kuri membrane chromatografiya mbere. Ikibaho gishobora gutwikirwa na
zahabu ya colloidal yanditseho anti cal McAb na zahabu ya colloidal yanditseho urukwavu IgG antibody mbere.
Iyo ugerageza icyitegererezo cyiza, inyana yicyitegererezo yahujwe na zahabu ya colloidal yanditseho anti cal McAb,
no gukora immunite igoye, nkuko byemewe kwimuka kuruhande rwibizamini, inyana ya conjugate
complexe ifatwa na anti cal coating McAb kuri membrane hanyuma ikora "anti cal coating
Zahabu ya McAb-cal-colloidal yanditseho anti cal McAb ”, itsinda ryibizamini byamabara ryagaragaye mubizamini
karere. Imbaraga zamabara zifitanye isano neza nibirimo inyana. Icyitegererezo kibi ntabwo
kora bande yikizamini kubera kubura colloidal zahabu conjugate cal complex. Ntakibazo cal
Kugaragara muri sample cyangwa sibyo, hari umurongo utukura ugaragara mukarere kerekana no kugenzura ubuziranenge
karere, bifatwa nkibipimo ngenderwaho byimishinga yimbere.

Ikizamini CAL ni uruganda rwambere rwabonye CFDA mubushinwa.Tumaze kohereza mubihugu byinshi hamwe nibisubizo byiza byose.

Murakaza neza kutwandikira kubindi bisobanuro.