Uruganda rukora Hp-ab antibody Indwara Byihuta Antibody Kumenya Ikizamini
Twibwira ko abakiriya batekereza, byihutirwa gukora bivuye mubyifuzo byumukiriya wumwanya wa théorie, kwemerera ubuziranenge bwiza, kugabanya ibiciro byo gutunganya, ibiciro byumvikana birumvikana cyane, byatsindiye abaguzi bashya kandi bishaje inkunga no kwemeza Gukora Ibigo byindwara ya CORONa Virusi Yihuta Yibizamini bya Antibody, Reka dufatanye hamwe kugirango dufatanye gukora ibyiza biri imbere. Twishimiye cyane gusura uruganda rwacu cyangwa kutuvugisha ubufatanye!
Twibwira icyo abakiriya batekereza, byihutirwa gukora bivuye mubyifuzo byumukiriya wimyumvire, kwemerera ubuziranenge bwiza, kugabanya ibiciro byo gutunganya, ibiciro byumvikana cyane, byatsindiye abaguzi bashya kandi bishaje inkunga no kubyemezaUbushinwa Ikizamini cya Antibody hamwe nigitabo cyo gupima, Buri gihe dukurikiza ihame ryisosiyete "inyangamugayo, inararibonye, ikora neza kandi igashya", hamwe ninshingano za: reka abashoferi bose bishimira gutwara ibinyabiziga nijoro, reka abakozi bacu bamenye agaciro kabo mubuzima, kandi bakomere kandi bakorere abantu benshi. Twiyemeje kuba intangiriro yisoko ryibicuruzwa byacu hamwe na serivise imwe itanga isoko ryibicuruzwa byacu.
Igikoresho cyo Gusuzuma Antibody kuri Helicobacter Pylori(Fluorescence Immunochromatographic Assay)
Kuri vitro kwisuzumisha koresha gusa
Nyamuneka soma iyi paki shyiramo witonze mbere yo gukoresha kandi ukurikize neza amabwiriza. Ibisubizo byizewe ntibishobora kwemezwa niba hari gutandukana kwamabwiriza muriyi paki yinjizamo.
GUKORESHA
Igikoresho cyo gusuzuma Antibody kuri Helicobacter Pylori (Fluorescence Immunochromatographic Assay) ni fluorescence immunochromatographic assay yo kumenya umubare wa antibody ya HP muri serumu cyangwa plasma. ningirakamaro yingirakamaro yo kwisuzumisha agaciro kwandura gastric.Icyitegererezo cyiza cyose kigomba kwemezwa nubundi buryo. Iki kizamini kigenewe ubuvuzi bwumwuga gusa.
INCAMAKE
Indwara ya Gastric helicobacter pylori ifitanye isano rya bugufi na gastrite idakira, ibisebe byo mu gifu, adenocarcinoma gastric, mucosa gastrica ifitanye isano na lymphoma, Hp ylori yanduye hafi 90% muri gastrite, ibisebe byo mu gifu, ibisebe byo mu nda no ku barwayi ba kanseri yo mu gifu. Ninde wamenye h. pylori nkubwoko bwa mbere bwibintu bitera kanseri.ni ibintu bishobora gutera kanseri yo mu gifu.H.pylori gutahura bifite agaciro gakomeye mugupima h. kwandura pylori.
IHame RY'UBURYO
Ibice by'igikoresho cyo kwipimisha bisizwe hamwe na antigen ya HP mu karere k'ibizamini hamwe n'ihene irwanya urukwavu IgG antibody ku karere kayobora. Lable pad isizwe na fluorescence yanditseho HP antigen ninkwavu IgG mbere. Iyo ugerageje icyitegererezo cyiza, antibody ya HP murugero ihuza na fluorescence yanditseho HP antigen, hanyuma ikora imvange yumubiri. Mubikorwa bya immunochromatografiya, urujya n'uruza rugana mu cyerekezo cyimpapuro zinjira, iyo complexe yatsinze akarere kizamini, ihuza na HP coating antigen, ikora urwego rushya.Urwego rwa HP-Ab rufitanye isano neza nibimenyetso bya fluorescence, hamwe no kwibanda kwa HP-Ab murugero irashobora gutahurwa na fluorescence immunoassay assay
REAGENTS N'IBIKORWA BYUZUWE
25T ibice:
Ikarita yikizamini kugiti cyayo ifatanye na desiccant 25T
Icyitegererezo cya 25T
Shyiramo paki 1
IBIKORWA BISABWA ARIKO NTIBITANZWE
Icyitegererezo cyo gukusanya ibikoresho, igihe
GUKORANYA URUGERO N'UBubiko
1.Ingero zapimwe zishobora kuba serumu, heparin anticoagulant plasma cyangwa plasma ya EDTA anticoagulant.
2.Kurikije tekiniki zisanzwe zegeranya icyitegererezo. Icyitegererezo cya serumu cyangwa plasma birashobora kubikwa muri firigo kuri 2-8 ℃ muminsi 7 hamwe no kubika munsi -15 ° C mumezi 6.
3.Icyitegererezo cyose wirinde gukonjesha.
GUKORA UBURYO
Nyamuneka soma igitabo gikoreshwa nigikoresho cyo gushyiramo mbere yo kugerageza.
1. Shira ku ruhande reagent zose hamwe nicyitegererezo kubushyuhe bwicyumba.
2.Fungura Portable Immune Analyser (WIZ-A101), andika ijambo ryibanga rya konte ukurikije uburyo bwo gukora igikoresho, hanyuma winjire muburyo bwo gutahura.
3.Shobora kode ya dentification kugirango wemeze ikintu cyizamini.
4.Kuramo ikarita yikizamini mu mufuka wa file.
5. Shyiramo ikarita yikizamini ahantu h'ikarita, suzuma kode ya QR, hanyuma umenye ikintu cyizamini.
6. Ongeramo 20μL serumu cyangwa plasma icyitegererezo kugirango utange urugero, hanyuma uvange neza ..
7. Ongeraho 80μL igisubizo cyicyitegererezo kugirango utange neza ikarita.
8.Kanda buto ya "ikizamini gisanzwe", nyuma yiminota 15, igikoresho kizahita kimenya ikarita yikizamini, irashobora gusoma ibisubizo bivuye kuri ecran yerekana igikoresho, hanyuma wandike / wandike ibisubizo byikizamini.
9. Reba ku mabwiriza ya Portable Immune Analyser (WIZ-A101).
AGACIRO KATEGANYIJWE
HP-Ab <10
Birasabwa ko buri laboratoire ishyiraho urwego rwayo rusanzwe ruhagarariye abaturage barwaye.
IBISUBIZO BY'IKIZAMINI NO GUSOBANURA
.Amakuru yavuzwe haruguru ni ibisubizo by'ikizamini cya HP-Ab, kandi hasabwa ko buri laboratoire igomba gushyiraho indangagaciro zitandukanye zo kumenya HP-Ab zibereye abaturage bo muri aka karere. Ibisubizo byavuzwe haruguru nibisobanuro gusa.
.Ibisubizo byubu buryo birakoreshwa gusa murwego rwoherejwe rwashyizweho muri ubu buryo, kandi ntaho bihuriye nubundi buryo.
.Ibindi bintu birashobora kandi gutera amakosa mubisubizo byo gutahura, harimo impamvu za tekiniki, amakosa yimikorere nibindi bintu byintangarugero.
Ububiko N'UBUHAMYA
1.Ibikoresho ni amezi 18 yo kubaho-uhereye igihe byakorewe. Bika ibikoresho bidakoreshwa kuri 2-30 ° C. NTUBUNTU. Ntugakoreshe kurenza itariki izarangiriraho.
2.Ntukingure umufuka ufunze kugeza igihe witeguye gukora ikizamini, kandi ikizamini kimwe cyo gukoreshwa gisabwa gukoreshwa mubisabwa (ubushyuhe 2-35 ℃, ubuhehere 40-90%) muminota 60 byihuse. bishoboka.
3.Urugero rwa diluent rukoreshwa ako kanya nyuma yo gufungura.
UMUBURO N'UBWITONDERWA
.Ibikoresho bigomba gufungwa kandi bikarindwa ubushuhe.
.Ingero zose nziza zemezwa nubundi buryo.
.Ingero zose zifatwa nkibishobora guhumanya.
.Ntugakoreshe reagent yarangiye.
.NtUGASIMBURE reagent mubikoresho bifite ubufindo butandukanye Oya ..
.NtUGASUBIZE gukoresha amakarita yikizamini nibikoresho byose bikoreshwa.
.Gukoresha nabi, urugero rwinshi cyangwa ruto rushobora kuganisha ku gutandukana.
LKwigana
.Nkuko hamwe nubushakashatsi ubwo aribwo bwose bukoresha antibodi yimbeba, birashoboka ko habaho kwivanga na antibodiyite zabantu barwanya imbeba (HAMA) murugero. Ingero z'abarwayi bahawe imyiteguro ya antibodiyite ya monoclonal yo gusuzuma cyangwa kuvura irashobora kuba irimo HAMA. Ingero nkizo zishobora gutera ibinyoma byiza cyangwa ibinyoma bibi.
.Ibisubizo by'ibizamini ni ibyerekeye ivuriro gusa, ntibigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura kwa muganga, imicungire y’amavuriro y’abarwayi igomba kwitabwaho byimazeyo hamwe n’ibimenyetso byayo, amateka y’ubuvuzi, ibindi bizamini bya laboratoire, igisubizo cy’ubuvuzi, epidemiologiya nandi makuru .
.Iyi reagent ikoreshwa gusa mugupima serumu na plasma. Ntishobora kubona ibisubizo nyabyo mugihe ikoreshejwe izindi ngero nka macandwe ninkari nibindi.
IBIKORWA BIKORWA
Umurongo | 10-1000 | gutandukana ugereranije: -15% kugeza kuri + 15%. |
Coefficient ihuza umurongo: (r) ≥0.9900 | ||
Ukuri | Igipimo cyo gukira kizaba kiri muri 85% - 115%. | |
Gusubiramo | CV≤15% |
RINGARUKA
1.Shao, JL & F.Wu.Iterambere rya vuba muburyo bwo gutahura Helicobacter pylori [J] .Ikinyamakuru cya Gastroenterology na Hepatology, 2012,21 (8): 691-694
2.Hansen JH, n'abandi. HAMA Kwivanga kwa Murine Monoclonal Antibody ishingiye kuri Immunoassays [J] .J ya Clin Immunoassay, 1993,16: 294-299.
3.Levinson S.
Urufunguzo rwibimenyetso byakoreshejwe:
Mubikoresho byubuvuzi bya Vitro | |
Uruganda | |
Ubike kuri 2-30 ℃ | |
Itariki izarangiriraho | |
Ntugakoreshe | |
ICYITONDERWA | |
Baza Amabwiriza yo Gukoresha |
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