IHame

Ibice by'igikoresho cyo kwipimisha bisizwe na antibody irwanya EV71 mu karere k'ibizamini hamwe na ihene irwanya urukwavu IgG antibody mu karere kayobora. Lable pad yashizwemo na fluorescence yanditseho antibody ya EV71 hamwe ninkwavu IgG mbere. Iyo ugerageza icyitegererezo cyiza, antigen ya EV71 murugero ikomatanya na fluorescence yanditseho antibody ya anti EV71, hanyuma igakora imvange yumubiri. Mubikorwa bya chromatografiya, urujya n'uruza rugana mu cyerekezo cyimpapuro zinjira, iyo complexe yatsinze akarere k'ibizamini, yahujwe na antibody ya anti-EV71, ikora urwego rushya.

Niba ari bibi, icyitegererezo ntikirimo enterovirus 71 IgM antibody, kugirango ubudahangarwa bw'umubiri budashobora kubaho. Nta murongo utukura uzaba ahantu hagaragara (T). Ntakibazo cyaba antibody ya Enterovirus 71 IgM ibaho murugero cyangwa ntihabeho, ibisigaye bya zahabu byanditseho imbeba birwanya antibody ya muntu IgM monoclonal antibody na antibody y'ihene irwanya imbeba IgG ikozweho ahantu hagenzurwa ubuziranenge (C). Hanyuma agglutinates itezimbere ibara mugace kagenzura ubuziranenge, kandi umurongo utukura uzagaragara muri (C). Umurongo utukura nicyo gisanzwe kigaragara ahantu hagenzurwa ubuziranenge (C) kugirango harebwe niba hari ingero zihagije kandi niba inzira ya chromatografiya ari ibisanzwe. Irakoreshwa kandi nkigenzura ryimbere kuri reagent.

Uburyo bw'ikizamini:

1.Ingero zapimwe zishobora kuba amaraso yose, harimo amaraso y'amaraso cyangwa amaraso ya Periferiya. Amaraso yose ntashobora kubikwa nyuma yo gukusanya. Nkwiye gukoreshwa vuba nyuma yo gukusanya.

2.Urugero rwa serumu rwakusanyirijwe hamwe ukurikije tekiniki zisanzwe. Serumu idashyushye ntishobora gukoresha. Ntabwo byemewe gukoresha lipemic, turbid cyangwa serumu yanduye. Sobanura ibintu muri serumu. Kandi imvura izagira ingaruka kubisubizo byikizamini, ingero zigomba kuba zashyizwe hagati cyangwa zungururwa mbere yo gukoreshwa.

3.Urugero rwapimwe rushobora kuba heparin, Sodium citrate cyangwa plasma ya EDTA anticoagulant.

4. Ukurikije tekinike isanzwe ikusanya icyitegererezo. Icyitegererezo cya serumu cyangwa plasma birashobora kubikwa muri firigo kuri 2-8 ℃ muminsi 3 hamwe no kubika munsi -15 ° C mumezi 3.

5.Icyitegererezo cyose wirinde gukonjesha.