IHame

Ibice byibikoresho byipimishije bisizwe hamwe na antivine ya Rotavirus A mugace k’ibizamini hamwe na antibody yihene irwanya urukwavu IgG mukarere kayobora. Lable pad yashizwemo na fluorescence yanditseho anti Rotavirus Itsinda A hamwe ninkwavu IgG mbere. Iyo ugerageza icyitegererezo cyiza, RV murugero ihuza hamwe na fluorescence yanditseho anti Rotavirus Group A, hanyuma igakora imvange yubudahangarwa. Mubikorwa bya immunochromatografiya, urujya n'uruza rwerekezo rwimpapuro zinjira. Iyo complexe yatsinze akarere k'ibizamini, yahujwe na anti-Rotavirus Group A coating antibody, ikora urwego rushya. Niba ari bibi, nta antigen ya Groupe A ya antavine iri murugero, kugirango ingirabuzimafatizo zidashobora gushingwa, ntihazabaho umurongo utukura ahantu hagaragara (T). Hatitawe ku kumenya niba itsinda A rotavirus ihari murugero, imbeba ya latex yanditseho imbeba IgG ni chromatografiya mugace kagenzura ubuziranenge (C) kandi igafatwa na antibody y'ihene irwanya imbeba. Umurongo utukura uzagaragara mugace kagenzura ubuziranenge (C). Umurongo utukura nicyo gisanzwe kigaragara ahantu hagenzurwa ubuziranenge (C) kugirango harebwe niba hari ingero zihagije kandi niba inzira ya chromatografiya ari ibisanzwe. Irakoreshwa kandi nkigenzura ryimbere kuri reagent.

Uburyo bw'ikizamini:

1.Abarwayi bafite ibimenyetso bagomba gukusanywa. Nk’uko amakuru abitangaza, gusohora kwinshi kwa rotavirus mu mwanda w’abarwayi barwaye gastroenteritis bibaho nyuma yiminsi 3-5 nyuma yindwara itangiye niminsi 3-13 nyuma yo gutangira ibimenyetso. Niba icyitegererezo cyegeranijwe nyuma yigihe cyo gucibwamo, umubare wa antigene ntushobora kuba uhagije kugirango habeho reaction nziza.

2.Icyitegererezo kigomba gukusanyirizwa mu kintu gisukuye, cyumye, kitarimo amazi kitarimo ibintu byangiza.

3.Ku barwayi badafite impiswi, ingero zanduye zegeranijwe ntizigomba kuba munsi ya garama 1-2. Ku barwayi barwaye impiswi, niba umwanda utemba, nyamuneka gukusanya byibuze ml 1-2 y'amazi. Niba umwanda urimo amaraso menshi na mucus, nyamuneka ongera ukusanye icyitegererezo.

4.Birasabwa gupima ibyitegererezo nyuma yo gukusanya, bitabaye ibyo bigomba koherezwa muri laboratoire mu masaha 6 bikabikwa kuri 2-8 ° C. Niba ingero zitarageragejwe mu masaha 72, zigomba kubikwa ku bushyuhe buri munsi ya 15 ° C.

5.Koresha umwanda mushya wo kwipimisha, hamwe nicyitegererezo cyimyanda ivanze namazi meza