Igikoresho cyo gusuzuma kuri Thyroxine Yuzuye (fluorescence immunochromatographic assay)
Kuri vitro kwisuzumisha koresha gusa
Nyamuneka soma iyi paki shyiramo witonze mbere yo gukoresha kandi ukurikize neza amabwiriza. Ibisubizo byizewe ntibishobora kwemezwa niba hari gutandukana kwamabwiriza muriyi paki yinjizamo.

GUKORESHA
Igikoresho cyo kwisuzumisha kuri Thyroxine Yuzuye (fluorescence immunochromatographic assay) ni fluorescence immunochromatographic assay yo kumenya umubare wuzuye wa Thyroxine (TT4) muri serumu yumuntu cyangwa plasma, ikoreshwa cyane mugusuzuma imikorere ya tiroyide.Ni urugero rwiza rwo gusuzuma indwara. bigomba kwemezwa nubundi buryo. Iki kizamini kigenewe ubuvuzi bwumwuga gusa.

INCAMAKE
Thyroxine (T4) isohorwa na glande ya tiroyide kandi uburemere bwayo ni 777D. Igiteranyo cya T4 (Igiteranyo cya T4, TT4) muri serumu nikubye inshuro 50 za serumu T3. Muri byo, 99,9% ya TT4 ihuza serumu Thyroxine Binding Proteins (TBP), naho T4 yubuntu (T4, FT4) iri munsi ya 0.05%. T4 na T3 bigira uruhare mugutunganya imikorere yumubiri. Ibipimo bya TT4 bikoreshwa mugusuzuma imikorere ya tiroyide no gusuzuma indwara. Mubuvuzi, TT4 ni ikimenyetso cyizewe cyo gusuzuma no gusuzuma neza hyperthyroidism na hypotherroidism.

IHame RY'UBURYO
Membrane yibikoresho bipimisha yashizwemo na conjugate ya BSA na T4 mukarere kipimishije hamwe na ihene irwanya urukwavu IgG antibody mukarere kayobora. Ikimenyetso cya marike gitwikiriwe na fluorescence ikimenyetso kirwanya antibody ya T4 ninkwavu IgG mbere. Iyo ugerageza icyitegererezo, TT4 muri sample ikomatanya na fluorescence yaranze antibody ya T4, hanyuma igakora imvange yumubiri. Mubikorwa bya immunochromatografiya, urujya n'uruza rugana mu cyerekezo cyimpapuro zinjira, iyo complexe yatsinze akarere kizamini, Ikimenyetso cya fluorescent yubusa kizahuzwa na T4 kuri membrane.Ubushuhe bwa TT4 ni ihuriro ribi kubimenyetso bya fluorescence, na kwibumbira hamwe kwa TT4 murugero birashobora gutahurwa na fluorescence immunoassay assay.

REAGENTS N'IBIKORWA BYUZUWE

25T ibice：
.Ikarita yipimisha kugiti cyawe ifu yometse kuri desiccant 25T
.Umuti 25T
.B igisubizo 1
.Paki yinjizamo 1

IBIKORWA BISABWA ARIKO NTIBITANZWE
Icyitegererezo cyo gukusanya ibikoresho, igihe

GUKORANYA URUGERO N'UBubiko
1.Ingero zapimwe zishobora kuba serumu, heparin anticoagulant plasma cyangwa plasma ya EDTA anticoagulant.
2.Kurikije tekiniki zisanzwe zegeranya icyitegererezo. Icyitegererezo cya serumu cyangwa plasma birashobora kubikwa muri firigo kuri 2-8 ℃ muminsi 7 hamwe no kubika munsi -15 ° C mumezi 6.
3.Icyitegererezo cyose wirinde gukonjesha.

GUKORA UBURYO

Uburyo bwo gupima igikoresho reba igitabo gikingira immunoanalyser. Uburyo bwo kwipimisha reagent nuburyo bukurikira
1. Shira ku ruhande reagent zose hamwe nicyitegererezo kubushyuhe bwicyumba.
2.Fungura Portable Immune Analyser (WIZ-A101), andika ijambo ryibanga rya konte ukurikije uburyo bwo gukora igikoresho, hanyuma winjire muburyo bwo gutahura.
3.Shobora kode ya dentification kugirango wemeze ikintu cyizamini.
3.Kuramo ikarita yikizamini mu gikapu.
4. Shyiramo ikarita yikizamini mumwanya wikarita, suzuma kode ya QR, hanyuma umenye ikintu cyizamini.
5. Ongeraho 20μL serumu cyangwa plasma icyitegererezo kubisubizo, hanyuma uvange neza.
6. Ongeraho 20μL B igisubizo kivanze hejuru, hanyuma uvange neza.

Kureka imvange ya20iminota.
Ongeramo 80μL ivanze kugirango utange neza ikarita.
Kanda buto ya "test test", nyuma yiminota 10, igikoresho kizahita kimenya ikarita yikizamini, kirashobora gusoma ibisubizo bivuye kuri ecran yerekana igikoresho, hanyuma wandike / wandike ibisubizo byikizamini.
Reba amabwiriza ya Portable Immune Analyser (WIZ-A101).

AGACIRO KATEGANYIJWE
TT4 urwego rusanzwe: 55-140nmol / L.
Birasabwa ko buri laboratoire ishyiraho urwego rwayo rusanzwe ruhagarariye abaturage barwaye.

IBISUBIZO BY'IKIZAMINI NO GUSOBANURA
.Amakuru yavuzwe haruguru ni intera yerekanwe yashyizweho kugirango hamenyekane amakuru yiki gitabo, kandi birasabwa ko buri laboratoire igomba gushyiraho intera yerekana akamaro k’amavuriro y’abaturage muri kano karere.
.Ubushuhe bwa TT4 burenze urwego ruvugwa, kandi impinduka za physiologique cyangwa igisubizo cyibibazo bigomba kuvaho.Ibidasanzwe, bigomba guhuza ibimenyetso byo kwa muganga.
.Ibisubizo byubu buryo birakoreshwa gusa murwego rwoherejwe rwashyizweho nubu buryo, kandi ibisubizo ntabwo bigereranywa nubundi buryo.
.Ibindi bintu birashobora kandi gutera amakosa mubisubizo byo gutahura, harimo impamvu za tekiniki, amakosa yimikorere nibindi bintu byintangarugero.

Ububiko N'UBUHAMYA
.Ibikoresho ni amezi 18 yo kubaho-uhereye igihe byakorewe. Bika ibikoresho bidakoreshwa kuri 2-30 ° C. NTUBUNTU. Ntugakoreshe kurenza itariki izarangiriraho.
.Ntukingure umufuka ufunze kugeza igihe witeguye gukora ikizamini, kandi ikizamini kimwe cyo gukoreshwa kirasabwa gukoreshwa mubidukikije bisabwa (ubushyuhe 2-35 ℃, ubushuhe 40-90%) muminota 60 byihuse birashoboka.
.Urugero rwa diluent rukoreshwa ako kanya nyuma yo gufungura.

UMUBURO N'UBWITONDERWA
.Ibikoresho bigomba gufungwa kandi bikarindwa ubushuhe.
.Ingero zose nziza zemezwa nubundi buryo.
.Ingero zose zifatwa nkibishobora guhumanya.
.Ntugakoreshe reagent yarangiye.
.NtUGASIMBURE reagent mubikoresho bifite ubufindo butandukanye oya ..
.NtUGASUBIZE gukoresha amakarita yikizamini nibikoresho byose bikoreshwa.
.Gukoresha nabi, urugero rwinshi cyangwa ruto rushobora kuganisha ku gutandukana.

LKwigana
.Nkuko hamwe nubushakashatsi ubwo aribwo bwose bukoresha antibodi yimbeba, birashoboka ko habaho kwivanga na antibodiyite zabantu barwanya imbeba (HAMA) murugero. Ingero z'abarwayi bahawe imyiteguro ya antibodiyite ya monoclonal yo gusuzuma cyangwa kuvura irashobora kuba irimo HAMA. Ingero nkizo zishobora gutera ibinyoma byiza cyangwa ibinyoma bibi.
.Ibisubizo by'ibizamini ni ibyerekeye ivuriro gusa, ntibigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura kwa muganga, imicungire y’amavuriro y’abarwayi igomba kwitabwaho byimazeyo hamwe n’ibimenyetso byayo, amateka y’ubuvuzi, ibindi bizamini bya laboratoire, igisubizo cy’ubuvuzi, epidemiologiya nandi makuru .
.Iyi reagent ikoreshwa gusa mugupima serumu na plasma. Ntishobora kubona ibisubizo nyabyo mugihe ikoreshejwe izindi ngero nka macandwe ninkari nibindi.

IBIKORWA BIKORWA

RINGARUKA
1.Hansen JH, n'abandi.HAMA Kwivanga kwa Murine Monoclonal Antibody ishingiye kuri Immunoassays [J] .J ya Clin Immunoassay, 1993,16: 294-299.
2.Levinson S.

Urufunguzo rwibimenyetso byakoreshejwe:

	Mubikoresho byubuvuzi bya Vitro
	Uruganda
	Ubike kuri 2-30 ℃
	Itariki izarangiriraho
	Ntugakoreshe
	ICYITONDERWA
	Baza Amabwiriza yo Gukoresha

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