Gusuzuma ibikoresho bya tiroxine (Fluorescence Umungororomatografia Assay)
Kuberako muri vitro ikoresha gusa
Nyamuneka soma iyi paki witonze mbere yo gukoresha kandi ukurikize neza amabwiriza. Kwizerwa kw'ibisubizo Ibisubizo ntibishobora kwizerwa niba hari gutandukana mumabwiriza muriyi paki.

Gukoresha
Diagnostic kit kuri thyroxine yawe (fluorescence imyumechromatographic assuy) ni fluorescence imyume igomba gushimangirwa nubundi buryo. Iki kizamini gigenewe gukoreshwa numwuga gusa.

Incamake
Thyroxine (T4) ihinduka na glande ya tiroyide nuburemere bwayo bushimishije ni 777D. T4 yose (byose T4, TT4) muri serumu ni inshuro 50 za Serum T3. Muri bo, 99.9% ya TT4 bihuza na serum throxine ihuza poroteyine (tbp), n'ubuntu t4 (ubuntu t4, ft4) iri munsi ya 0.05%. T4 na T3 bitabira kugenzura imikorere yumubiri. Ibipimo bya TT4 bikoreshwa mugusuzuma imiterere ya tiroyide no gusuzuma indwara. Calnic, TT4 ni ikimenyetso cyizewe cyo kwisuzumisha hamwe nibikorwa byiringiro bya hyperthyroidism na hypothididism.

Ihame ry'uburyo
Igikoresho cy'ibizamini gikongejwe hamwe na CUS Conjugate ya BSA na T4 mu karere k'ikizamini n'ihene kurwanya urukwavu Igg antibod mu karere kagenzura. Marker Pad yashizwemo na fluorescence Mark Anti T4 antibody ninkwavu igg mbere. Iyo ugerageza icyitegererezo, TT4 muruziga ruhuza na fluorescence yashyizeho ikimenyetso cyo kurwanya anti t4, kandi ikora imvange. Munsi y'ibikorwa bya Imyumechromatography, urujya n'uruza rugana ku mpapuro zinjira, igihe bigoye kunyura muri T4 kuri membran.Ubushakashatsi bwa TT4 bufitanye isano n'ibimenyetso bibi, na Kwibanda kuri TT4 murugero birashobora kumenyekana na fluorescence imyumesas.

Reages nibikoresho byatanzwe

Ibikoresho 25t:
.Ikarita ikarita kugiti cye isenyutse hamwe na 25t
.Umuti wa 25t
.B Igisubizo 1
.Gukoreshamo ibice 1

Ibikoresho birakenewe ariko ntibitangwa
Icyitegererezo cyo gukusanya ibintu, igihe

Icyitegererezo cyo gukusanya no kubika
1.Icyitegererezo cyageragejwe gishobora kuba, Heparin anticogulant Plasma cyangwa EDTA Anticogulant Plasma.
2.Ubuhanga busanzwe bwo gukusanya icyitegererezo. Serum cyangwa Plasma Icyitegererezo gishobora gukomeza gukonjesha saa mbiri-8 ℃ ku minsi 7 na konko hepfo -15 ° C mumezi 6.
3.Icyitegererezo cyose irinde guhagarika inzinguzingo.

Uburyo bwo Gukwirakwiza

Uburyo bwikizamini mubikoresho reba igitabo cya Immwungowarzer. Uburyo bwikizamini cya reagent ni bukurikira
1.Ubundi ku ruhande reagents zose hamwe ningero kubushyuhe bwicyumba.
.
3.scan code yo gutesha agaciro kugirango yemeze ikintu cyibizamini.
3.Kuraho ikarita yikizamini kuva mu gikapu cya foil.
)
5.ADD 20μl serumu cyangwa icyitegererezo cya plasma kubisubizo, hanyuma uvange neza.
6.Add 20μl b igisubizo kivanze hejuru, hanyuma uvange neza.

Kureka imvange kuri20iminota.
Ongeramo 80μl imbogamizi kuri sample iriba ikarita.
Kanda buto ya "Ikizamini gisanzwe", nyuma yiminota 10, igikoresho kizahita kimenya ikarita yikizamini, irashobora gusoma ibisubizo bivuye kuri ecran yerekana igikoresho, hanyuma wandike / andika ibisubizo byikizamini.
Reba ku nyigisho z'abacunganzinguzi (Wiz-A101).

Indangagaciro ziteganijwe
TT4 intera isanzwe: 55-140nMol / l
Birasabwa ko buri laboratoire ishyiraho urwego rusanzwe rugereranya abarwayi bayo.

Ibisubizo n'ibisubizo
.Amakuru yavuzwe haruguru ni intera yerekanwe ku makuru yo kumenya ibi bikoresho, kandi birasabwa ko buri laboratoire ikwiye gushyiraho intera iboneye ku bijyanye n'ubushobozi bwa clinique y'abaturage bo muri kano karere.
.Ibitekerezo bya TT4 birenze intera yeguye, hamwe nimpinduka za physiologique cyangwa igisubizo cyo guhangayika bigomba kuvaho.
.Ibisubizo byubu buryo bikoreshwa gusa kumirongo yerekanwe yashizweho nubu buryo, kandi ibisubizo ntabwo bigereranywa nuburyo butaziguye nubundi buryo.
.Ibintu birashobora kandi gutera amakosa kubisubizo, harimo impamvu za tekiniki, amakosa yimikorere nibindi bintu byintangarugero.

Kubika no gutuza
.Ibikoresho bifite amezi 18 yuzuye - ubuzima kuva gukora. Bika ibikoresho bidakoreshwa kuri 2-30 ° C. Ntugahagarike. Ntukoreshe kurenza itariki izarangiriraho.
.Ntabwo ukingure umufuka ufunze kugeza witeguye gukora ikizamini, kandi ikizamini cyo gukoresha kimwe gisabwa gukoreshwa (ubushyuhe 2-35 ℃, ubushyuhe bwa 2-35 birashoboka.
.Sample Diluennt ikoreshwa ako kanya nyuma yo gufungura.

Umuburo n'inyungu
.Ibikoresho bigomba gushyirwaho ikimenyetso no gukingirwa ubushuhe.
.Ibikoresho byiza bigenwa nubundi buryo.
.Ibigereranyo bizafatwa nkuwanduye.
.Ntukoreshe reagent yarangiye.
.Ntabwo uhanagura reagent mubikoresho hamwe na love hatandukanye oya ..
.Do ntukoreshe amakarita y'ibizamini hamwe nibikoresho byose bitashoboka.
.

LKwigana
.Kuvuga hamwe nigipimo icyo aricyo cyose gikoresha imbeba antibolies, ibishoboka bibaho kubavanga numuntu arwanya imbeba (Hama) murugero. Ingendo zituruka ku barwayi babonye imyiteguro ya antibodis ya monoclonal yo kwisuzumisha cyangwa kuvura bishobora kuba birimo hama. Ibyingenzi birashobora gutera ibisubizo byiza cyangwa ibinyoma.
.Ibisubizo byibizamini bigamije kuvugwa gusa, ntibigomba kuba ishingiro ryonyine ryo gusuzuma amavuriro no kuvurwa, Amateka y'ubuvuzi, mu yandi mateka, igisubizo cyo kuvura nandi makuru .
.Ibi bikoreshwa gusa kubizamini bya Serum nibizamini bya Plasma. Ntabwo ishobora kubona ibisubizo nyabyo iyo bikoreshwa mubindi byitegererezo nkamacandwe ninkari n'ikibi nibindi.

Ibiranga imikorere

RIbisobanuro
.
2.Levinson S. Imiterere ya antibodique ninshingano muri Impungo zivanze [j] .J wa Clin Imyungesay, 1992: 108-114.

Urufunguzo rwibimenyetso byakoreshejwe:

	Mugikoresho cyo kwivuza kwa vitro
	Uruganda
	Ububiko kuri 2-30 ℃
	Itariki yo kurangiriraho
	Ntukoreshe
	Kwitondera
	Ngera inama amabwiriza yo gukoresha

Xiamen wiz biotech co., Ltd
Aderesi: Igorofa 3-4, No.16 Amahugurwa yubuvuzi, Amahugurwa yubuvuzi, 2030 Wengjiao Umuhanda Wiburengerazuba, Akarere ka Haicang, 361026, Xiamen, Abapayiniya
Tel: + 86-592-6808278
Fax: + 86-592-6808279