Igikoresho cyo Gusuzuma Progesterone (Fluorescence Imyunochromatograchic Assay)

Ibisobanuro bigufi:


  • Igihe cyo kugerageza:Iminota 10-15
  • Igihe cyemewe:Ukwezi 24
  • ICYITONDERWA:Kurenga 99%
  • Ibisobanuro:1/25 Ikizamini / Agasanduku
  • Ubushyuhe bwo kubika:2 ℃ -30 ℃
  • Ibisobanuro birambuye

    Ibicuruzwa

    Gusuzuma ibikoresho bya progesterone(Fluorescence Umungororomatografia Assay)
    Kuberako muri vitro ikoresha gusa

    Nyamuneka soma iyi paki witonze mbere yo gukoresha kandi ukurikize neza amabwiriza. Kwizerwa kw'ibisubizo Ibisubizo ntibishobora kwizerwa niba hari gutandukana mumabwiriza muriyi paki.

    Gukoresha
    Diagnostic Kit kuri ProgesSerone (Fluorescence Imyunochromatograchic Assay) ni fluorescence imyume . Iki kizamini gigenewe gukoreshwa numwuga gusa.

    Incamake
    Progesterone ni imisemburo yingenzi agira uruhare runini mumabwiriza yimihango kandi ni ngombwa mugukomeza gutwita. Icyifuzo cya Progesterone muri Serum cyayongereye vuba nyuma yo gutanga intanga. Nibimenyetso byizewe bya ovulation cyangwa kwaguka ovulation.

    Ihame ry'uburyo
    Ingano yikikoresho cyibizamini yashizwemo hamwe na BSA na Prog mukarere kizamini kandi ihene anti rabbit igg antibod mukarere. Marker Pad yashizwemo na fluorescence Mark Anti Prog Antibody ninkwavu Igg mbere. Iyo ugerageza icyitegererezo, prog in dople ihuza na fluorescence yashyizeho ikimenyetso cyo kurwanya anti prog, hanyuma ukore imvange. Munsi y'ibikorwa bya Immunochromatography, urujya n'uruza rugana ku mpapuro zinjira, igihe uruganda rukora ikizamini. Ikimenyetso cya Prog kirimo guhuza ibibanza ku kimenyetso cya Fluorescence, na Kwibanda kuri prog murugero birashobora kumenyekana na fluorescence imyumesas.

    Reages nibikoresho byatanzwe
    Ibikoresho 25t:

    Ikarita y'Ibizamini Umuntu ku giti cye Yakozwe na Desiccant 25t
    Icyitegererezo cya dilueds 25t
    Ipaki Yinjije 1

    Ibikoresho birakenewe ariko ntibitangwa
    Icyitegererezo cyo gukusanya ibintu, igihe

    Icyitegererezo cyo gukusanya no kubika
    1.Icyitegererezo cyageragejwe gishobora kuba, Heparin anticogulant Plasma cyangwa EDTA Anticogulant Plasma.

    2.Ubuhanga busanzwe bwo gukusanya icyitegererezo. Serum cyangwa Plasma Icyitegererezo gishobora gukomeza gukonjesha saa mbiri-8 ℃ ku minsi 7 na konko hepfo -15 ° C mumezi 6.
    3.Icyitegererezo cyose irinde guhagarika inzinguzingo.

    Uburyo bwo Gukwirakwiza
    Nyamuneka soma igitabo cyigikoresho cyigikoresho na paki shyiramo mbere yo kwipimisha.

    1.Ubundi ku ruhande reagents zose hamwe ningero kubushyuhe bwicyumba.
    2Ipey isesengura rya Portable Portezer (Wiz-A101), andika ijambo ryibanga rya konte ukurikije imikorere yikikoresho, hanyuma wandike interineti.
    3.scan code yo gutesha agaciro kugirango yemeze ikintu cyibizamini.
    4. Fata ikarita yikizamini kuva mu gikapu.
    5.Mugukanda ikarita yikizamini mubibanza byamakarita, scan code ya QR, hanyuma umenye ikintu cyibizamini.
    6.Add 20μl serumu cyangwa icyitegererezo cya plasma kuri sample dilunt, hanyuma uvange neza ..
    7.Add 80μl icyitegererezo igisubizo kuri sample iriba ikarita.
    8.Kwita "Ikizamini gisanzwe", nyuma yiminota 10, igikoresho kizahita kimenya ikarita yikizamini, birashobora gusoma ibisubizo bivuye mu kwerekana igikoresho, hanyuma wandike / andika ibisubizo byikizamini.
    9.Gukoresha inyigisho z'abacunganzida (Wiz-A101).

    Ibisubizo n'ibisubizo

    Icyiciro

    Intera (ng / ml)

    Umugabo

    0.1-0.9

    Igitsina gore

    Icyiciro cya Follicular / Igihe cya Ovulary

    0.3-1.5

    Icyiciro cya Luteal

    5.2-18.5

    Gucura

    <0.8

    .Amakuru yavuzwe haruguru ni ibisubizo byikizamini cyateganijwe, kandi birasabwa ko buri laboratoire igomba gushyiraho indangagaciro zidasanzwe zikwiranye nabaturage muri kano karere. Ibisubizo byavuzwe haruguru nibyo bivugwa gusa.
    .Ibisubizo byubu buryo bikoreshwa gusa kubijyanye ningingo zerekanwe muri ubu buryo, kandi ntagereranywa nubundi buryo.
    .Ibintu birashobora kandi gutera amakosa kubisubizo, harimo impamvu za tekiniki, amakosa yimikorere nibindi bintu byintangarugero.

    Kubika no gutuza
    1.Ibikoresho bifite amezi 18 yuzuye - ubuzima kuva gukora. Bika ibikoresho bidakoreshwa kuri 2-30 ° C. Ntugahagarike. Ntukoreshe kurenza itariki izarangiriraho.

    2.Ntukingure umufuka ufunze kugeza witeguye gukora ikizamini, kandi ikizamini cyo gukoresha kimwe gisabwa gukoreshwa (ubushyuhe 2-35 bishoboka.
    3Sample Dilunt ikoreshwa ako kanya nyuma yo gufungura.

    Umuburo n'inyungu
    .Ibikoresho bigomba gushyirwaho ikimenyetso no gukingirwa ubushuhe.

    .Ibikoresho byiza bigenwa nubundi buryo.
    .Ibigereranyo bizafatwa nkuwanduye.
    .Ntukoreshe reagent yarangiye.
    .Ntabwo uhanagura reagent mubikoresho hamwe na love hatandukanye oya ..
    .Do ntukoreshe amakarita y'ibizamini hamwe nibikoresho byose bitashoboka.
    .

    LKwigana
    .Kuvuga hamwe nigipimo icyo aricyo cyose gikoresha imbeba antibolies, ibishoboka bibaho kubavanga numuntu arwanya imbeba (Hama) murugero. Ingendo zituruka ku barwayi babonye imyiteguro ya antibodis ya monoclonal yo kwisuzumisha cyangwa kuvura bishobora kuba birimo hama. Ibyingenzi birashobora gutera ibisubizo byiza cyangwa ibinyoma.

    .Ibisubizo byibizamini bigamije kuvugwa gusa, ntibigomba kuba ishingiro ryonyine ryo gusuzuma amavuriro no kuvurwa, Amateka y'ubuvuzi, mu yandi mateka, igisubizo cyo kuvura nandi makuru .
    .Ibi bikoreshwa gusa kubizamini bya Serum nibizamini bya Plasma. Ntabwo ishobora kubona ibisubizo nyabyo iyo bikoreshwa mubindi byitegererezo nkamacandwe ninkari n'ikibi nibindi.

    Ibiranga imikorere

    Umurongo 0.5ng / ml kugeza kuri 50ng / ml gutandukana kwa ugereranije: -15% kugeza kuri 15%.
    Guhuza umurongo umurongo: (R) ≥0.9900
    Ukuri Igipimo cyo gukira kigomba kuba muri 85% - 115%.
    Gusubiramo CV≤15%
    Umwihariko.

    Invancerent

    Kwibanda

    E2

    500ng / ml

    T

    500ng / ml

    Cor

    500ng / ml

    E3

    100ng / ml

    17β-E2

    100ng / ml

    RIbisobanuro
    .

    2.Levinson S. Imiterere ya antibodique ninshingano muri Impungo zivanze [j] .J wa Clin Imyungesay, 1992: 108-114.

    Urufunguzo rwibimenyetso byakoreshejwe:

     t11-1 Mugikoresho cyo kwivuza kwa vitro
     TT-2 Uruganda
     TT-71 Ububiko kuri 2-30 ℃
     TT-3 Itariki yo kurangiriraho
     TT-4 Ntukoreshe
     TT-5 Kwitondera
     TT-6 Ngera inama amabwiriza yo gukoresha

    Xiamen wiz biotech co., Ltd
    Aderesi: Igorofa 3-4, No.16 Amahugurwa yubuvuzi, Amahugurwa yubuvuzi, 2030 Wengjiao Umuhanda Wiburengerazuba, Akarere ka Haicang, 361026, Xiamen, Abapayiniya
    Tel: + 86-592-6808278
    Fax: + 86-592-6808279


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