Igikoresho cyo gusuzumaLuteinizing Hormonefluorescence immunochromatographic assay)
Kuri vitro kwisuzumisha koresha gusa
Nyamuneka soma iyi paki shyiramo witonze mbere yo gukoresha kandi ukurikize neza amabwiriza. Ibisubizo byizewe ntibishobora kwemezwa niba hari gutandukana kwamabwiriza muriyi paki yinjizamo.

GUKORESHA
Igikoresho cyo gusuzuma indwara ya Luteinizing Hormone (fluorescence immunochromatographic assay) ni fluorescence immunochromatographic assay kugirango igaragaze umubare wa Luteinizing Hormone (LH) muri serumu yumuntu cyangwa plasma, ikoreshwa cyane mugusuzuma imikorere ya endocrine ya pitoito. Icyitegererezo cyiza cyose kigomba kwemezwa na ubundi buryo. Iki kizamini kigenewe ubuvuzi bwumwuga gusa.

INCAMAKE

Luteinizing hormone (LH) ni glycoproteine ​​ifite uburemere bwa molekile igera ku 30.000 Dalton, ikorwa na pitoito y'imbere. Ubwinshi bwa LH bufitanye isano rya hafi na ovulation yintanga, kandi impinga ya LH iteganijwe kuba amasaha 24 kugeza kuri 36 yintanga. Kubwibyo, agaciro ka LH gashobora gukurikiranwa mugihe cyimihango kugirango hamenyekane igihe cyiza cyo gusama. Imikorere idasanzwe ya endocrine muri glande ya pitoito irashobora gutera LH gusohora bidasanzwe.Ubushuhe bwa LH burashobora gukoreshwa mugusuzuma imikorere ya pitoito endocrine. Igikoresho cyo Gusuzuma gishingiye kuri immunochromatografiya kandi gishobora gutanga ibisubizo muminota 15.

IHame RY'UBURYO

Ibice by'igikoresho cyo kwipimisha bisizwe hamwe na antibody anti-LH mu karere k'ibizamini hamwe n'ihene irwanya urukwavu IgG antibody mu karere kayobora. Lable pad isizwe na fluorescence yanditseho antibody ya LH hamwe ninkwavu IgG mbere. Iyo ugerageza icyitegererezo cyiza, antigen ya LH muri sample ikomatanya na fluorescence yanditseho antibody ya LH, hanyuma igakora imvange yumubiri. Mubikorwa bya immunochromatografiya, urujya n'uruza rugana mu cyerekezo cyimpapuro zinjira, iyo complexe yatsinze akarere k’ibizamini, yahujwe na anti-LH coating antibody, ikora urwego rushya. Urwego rwa LH rufitanye isano neza na signal ya fluorescence, kandi ubunini bwa LH murugero burashobora gutahurwa na fluorescence immunoassay assay.

REAGENTS N'IBIKORWA BYUZUWE

25T ibice：

.Ikarita yipimisha kugiti cyawe ifu yometse kuri desiccant 25T
.Urugero rwimvugo
. Shyiramo paki

IBIKORWA BISABWA ARIKO NTIBITANZWE
Icyitegererezo cyo gukusanya ibikoresho, igihe

GUKORANYA URUGERO N'UBubiko
1.Ingero zapimwe zishobora kuba serumu, heparin anticoagulant plasma cyangwa plasma ya EDTA anticoagulant.
2.Kurikije tekiniki zisanzwe zegeranya icyitegererezo. Icyitegererezo cya serumu cyangwa plasma birashobora kubikwa muri firigo kuri 2-8 ℃ muminsi 7 hamwe no kubika munsi -15 ° C mumezi 6.
3.Icyitegererezo cyose wirinde gukonjesha.

GUKORA UBURYO

Uburyo bwo gupima igikoresho reba igitabo gikingira immunoanalyser. Uburyo bwo kwipimisha reagent nuburyo bukurikira
1. Shira ku ruhande reagent zose hamwe nicyitegererezo kubushyuhe bwicyumba.
2.Fungura Portable Immune Analyser (WIZ-A101), andika ijambo ryibanga rya konte ukurikije uburyo bwo gukora igikoresho, hanyuma winjire muburyo bwo gutahura.
3.Shobora kode ya dentification kugirango wemeze ikintu cyizamini.
4.Kuramo ikarita yikizamini mu mufuka wa file.
5. Shyiramo ikarita yikizamini ahantu h'ikarita, suzuma kode ya QR, hanyuma umenye ikintu cyizamini.
6. Ongeramo 20μL serumu cyangwa plasma icyitegererezo kugirango utange urugero, hanyuma uvange neza ..
7. Ongeraho 80μL igisubizo cyicyitegererezo kugirango utange neza ikarita.
8.Kanda buto ya "ikizamini gisanzwe", nyuma yiminota 15, igikoresho kizahita kimenya ikarita yikizamini, irashobora gusoma ibisubizo bivuye kuri ecran yerekana igikoresho, hanyuma wandike / wandike ibisubizo byikizamini.
9. Reba ku mabwiriza ya Portable Immune Analyser (WIZ-A101).

IBISUBIZO BY'IKIZAMINI NO GUSOBANURA

.Amakuru yavuzwe haruguru ni intera yerekanwe yashyizweho kugirango hamenyekane amakuru yiki gitabo, kandi birasabwa ko buri laboratoire igomba gushyiraho intera yerekana akamaro k’amavuriro y’abaturage muri kano karere.
.Ubushuhe bwa LH burenze urwego ruvugwa, kandi impinduka zifatika cyangwa ibisubizo byimyitwarire bigomba kuvaho.Ibidasanzwe bidasanzwe, bigomba guhuza ibimenyetso byo kwa muganga.
.Ibisubizo byubu buryo birakoreshwa gusa murwego rwoherejwe rwashyizweho nubu buryo, kandi ibisubizo ntabwo bigereranywa nubundi buryo.
.Ibindi bintu birashobora kandi gutera amakosa mubisubizo byo gutahura, harimo impamvu za tekiniki, amakosa yimikorere nibindi bintu byintangarugero.

Ububiko N'UBUHAMYA
1.Ibikoresho ni amezi 18 yo kubaho-uhereye igihe byakorewe. Bika ibikoresho bidakoreshwa kuri 2-30 ° C. NTUBUNTU. Ntugakoreshe kurenza itariki izarangiriraho.
2.Ntukingure umufuka ufunze kugeza igihe witeguye gukora ikizamini, kandi ikizamini kimwe cyo gukoreshwa gisabwa gukoreshwa mubisabwa (ubushyuhe 2-35 ℃, ubuhehere 40-90%) muminota 60 byihuse. bishoboka.
3.Urugero rwa diluent rukoreshwa ako kanya nyuma yo gufungura.

UMUBURO N'UBWITONDERWA
.Ibikoresho bigomba gufungwa kandi bikarindwa ubushuhe.
.Ingero zose nziza zemezwa nubundi buryo.
.Ingero zose zifatwa nkibishobora guhumanya.
.Ntugakoreshe reagent yarangiye.
. SI guhinduranya reagent mubikoresho bifite ubufindo butandukanye Oya ..
.NtUGASUBIZE gukoresha amakarita yikizamini nibikoresho byose bikoreshwa.
.Gukoresha nabi, urugero rwinshi cyangwa ruto rushobora kuganisha ku gutandukana.

LKwigana
.Nkuko hamwe nubushakashatsi ubwo aribwo bwose bukoresha antibodi yimbeba, birashoboka ko habaho kwivanga na antibodiyite zabantu barwanya imbeba (HAMA) murugero. Ingero z'abarwayi bahawe imyiteguro ya antibodiyite ya monoclonal yo gusuzuma cyangwa kuvura irashobora kuba irimo HAMA. Ingero nkizo zishobora gutera ibinyoma byiza cyangwa ibinyoma bibi.
.Ibisubizo by'ibizamini ni ibyerekeye ivuriro gusa, ntibigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura kwa muganga, imicungire y’amavuriro y’abarwayi igomba kwitabwaho byimazeyo hamwe n’ibimenyetso byayo, amateka y’ubuvuzi, ibindi bizamini bya laboratoire, igisubizo cy’ubuvuzi, epidemiologiya nandi makuru .
.Iyi reagent ikoreshwa gusa mugupima serumu na plasma. Ntishobora kubona ibisubizo nyabyo mugihe ikoreshejwe izindi ngero nka macandwe ninkari nibindi.

IBIKORWA BIKORWA

INGINGO
1.Hansen JH, n'abandi.HAMA Kwivanga kwa Murine Monoclonal Antibody ishingiye kuri Immunoassays [J] .J ya Clin Immunoassay, 1993,16: 294-299.
2.Levinson S.

Urufunguzo rwibimenyetso byakoreshejwe:

	Mubikoresho byubuvuzi bya Vitro
	Uruganda
	Ubike kuri 2-30 ℃
	Itariki izarangiriraho
	Ntugakoreshe
	ICYITONDERWA
	Baza Amabwiriza yo Gukoresha

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