Gusuzuma Igikoresho cya Isoenzyme MB ya Creatine Kinase(fluorescence immunochromatographic assay)
Kuri vitro kwisuzumisha koresha gusa
Nyamuneka soma iyi paki shyiramo witonze mbere yo gukoresha kandi ukurikize neza amabwiriza. Ibisubizo byizewe ntibishobora kwemezwa niba hari gutandukana kwamabwiriza muriyi paki yinjizamo.

GUKORESHA
Igikoresho cyo gusuzuma kuri Isoenzyme MB ya Creatine Kinase (fluorescence immunochromatographic assay) ni fluorescence immunochromatographic assay yo kumenya umubare wa Isoenzyme MB ya Creatine Kinase (CK-MB) muri serumu cyangwa plasma, ikoreshwa mugupima ubufasha bwa AMI (Acute Myocardial) Infarction). Icyitegererezo cyiza cyose kigomba kwemezwa nubundi buryo. Iki kizamini kigenewe ubuvuzi bwumwuga gusa.

INCAMAKE
Urwego rwa CK-MB rwiyongereye nyuma yamasaha 4-6 nyuma yindwara ya Myocardial ibaye, igera kumasaha 18-24, hanyuma isubira mubisanzwe nyuma yiminsi 2-3. CK-MB irekura ikwirakwizwa nyuma ya hemoglobine.CK-MB ni ikimenyetso gakondo cyindwara ya myocardial acute.

IHame RY'UBURYO
Ibice by'igikoresho cyo kwipimisha bisizwe na antibody anti-CK-MB mu karere k’ibizamini hamwe na antibody y'ihene irwanya urukwavu IgG mu karere kayobora. Lable pad isizwe na fluorescence yanditseho antibody ya CK-MB hamwe ninkwavu IgG mbere. Iyo ugerageza icyitegererezo cyiza, antigen ya CK-MB muri sample ikomatanya na fluorescence yanditseho antibody ya CK-MB, hanyuma igakora imvange yumubiri. Mubikorwa bya immunochromatografiya, urujya n'uruza rugana mu cyerekezo cyimpapuro zinjira, iyo complexe yatsinze akarere k’ibizamini, yahujwe na antibody yo kurwanya CK-MB, ikora urwego rushya. Urwego rwa CK-MB rufitanye isano neza na signal ya fluorescence, kandi ubunini bwa CK-MB murugero burashobora gutahurwa na fluorescence immunoassay assay.

REAGENTS N'IBIKORWA BYUZUWE

25T ibice：
Ikarita yikizamini kugiti cyayo ifatanye na desiccant 25T
Icyitegererezo cya 25T
Shyiramo paki 1

IBIKORWA BISABWA ARIKO NTIBITANZWE
Icyitegererezo cyo gukusanya ibikoresho, igihe

GUKORANYA URUGERO N'UBubiko
1.Ingero zapimwe zishobora kuba serumu, heparin anticoagulant plasma cyangwa plasma ya EDTA anticoagulant.
2.Kurikije tekiniki zisanzwe zegeranya icyitegererezo. Icyitegererezo cya serumu cyangwa plasma birashobora kubikwa muri firigo kuri 2-8 ℃ muminsi 7 hamwe no kubika munsi -15 ° C mumezi 6.
3.Icyitegererezo cyose wirinde gukonjesha.

GUKORA UBURYO
Nyamuneka soma igitabo gikoreshwa nigikoresho cyo gushyiramo mbere yo kugerageza.
1. Shira ku ruhande reagent zose hamwe nicyitegererezo kubushyuhe bwicyumba.
2.Fungura Portable Immune Analyser (WIZ-A101), andika ijambo ryibanga rya konte ukurikije uburyo bwo gukora igikoresho, hanyuma winjire muburyo bwo gutahura.
3.Shobora kode ya dentification kugirango wemeze ikintu cyizamini.
4.Kuramo ikarita yikizamini mu mufuka wa file.
5. Shyiramo ikarita yikizamini ahantu h'ikarita, suzuma kode ya QR, hanyuma umenye ikintu cyizamini.
5. Ongeramo 40μL serumu cyangwa plasma icyitegererezo kugirango utange urugero, hanyuma uvange neza ..
6. Ongeraho 80μL icyitegererezo kugirango ubone icyitegererezo cyikarita.
7.Kanda buto ya "test test", nyuma yiminota 15, igikoresho kizahita kimenya ikarita yikizamini, kirashobora gusoma ibisubizo bivuye kuri ecran yerekana igikoresho, hanyuma wandike / wandike ibisubizo byikizamini.
8.Reba amabwiriza ya Portable Immune Analyser (WIZ-A101).

AGACIRO KATEGANYIJWE
CK-MB <5ng / mL
Birasabwa ko buri laboratoire ishyiraho urwego rwayo rusanzwe ruhagarariye abaturage barwaye.

IBISUBIZO BY'IKIZAMINI NO GUSOBANURA
.Amakuru yavuzwe haruguru ni ibisubizo byikizamini cya CK-MB, kandi hasabwa ko buri laboratoire igomba gushyiraho indangagaciro za CK-MB zerekana neza abaturage muri kano karere. Ibisubizo byavuzwe haruguru nibisobanuro gusa.
.Ibisubizo byubu buryo birakoreshwa gusa murwego rwoherejwe rwashyizweho muri ubu buryo, kandi ntaho bihuriye nubundi buryo.
.Ibindi bintu birashobora kandi gutera amakosa mubisubizo byo gutahura, harimo impamvu za tekiniki, amakosa yimikorere nibindi bintu byintangarugero.

Ububiko N'UBUHAMYA
1.Ibikoresho ni amezi 18 yo kubaho-uhereye igihe byakorewe. Bika ibikoresho bidakoreshwa kuri 2-30 ° C. NTUBUNTU. Ntugakoreshe kurenza itariki izarangiriraho.
2.Ntukingure umufuka ufunze kugeza igihe witeguye gukora ikizamini, kandi ikizamini kimwe cyo gukoreshwa gisabwa gukoreshwa mubisabwa (ubushyuhe 2-35 ℃, ubuhehere 40-90%) muminota 60 byihuse. bishoboka.
3.Urugero rwa diluent rukoreshwa ako kanya nyuma yo gufungura.

UMUBURO N'UBWITONDERWA
.Ibikoresho bigomba gufungwa kandi bikarindwa ubushuhe.
.Ingero zose nziza zemezwa nubundi buryo.
.Ingero zose zifatwa nkibishobora guhumanya.
.Ntugakoreshe reagent yarangiye.
.NtUGASIMBURE reagent mubikoresho bifite ubufindo butandukanye Oya ..
.NtUGASUBIZE gukoresha amakarita yikizamini nibikoresho byose bikoreshwa.
.Gukoresha nabi, urugero rwinshi cyangwa ruto rushobora kuganisha ku gutandukana.

LKwigana
.Nkuko hamwe nubushakashatsi ubwo aribwo bwose bukoresha antibodi yimbeba, birashoboka ko habaho kwivanga na antibodiyite zabantu barwanya imbeba (HAMA) murugero. Ingero z'abarwayi bahawe imyiteguro ya antibodiyite ya monoclonal yo gusuzuma cyangwa kuvura irashobora kuba irimo HAMA. Ingero nkizo zishobora gutera ibinyoma byiza cyangwa ibinyoma bibi.
.Ibisubizo by'ibizamini ni ibyerekeye ivuriro gusa, ntibigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura kwa muganga, imicungire y’amavuriro y’abarwayi igomba kwitabwaho byimazeyo hamwe n’ibimenyetso byayo, amateka y’ubuvuzi, ibindi bizamini bya laboratoire, igisubizo cy’ubuvuzi, epidemiologiya nandi makuru .
.Iyi reagent ikoreshwa gusa mugupima serumu na plasma. Ntishobora kubona ibisubizo nyabyo mugihe ikoreshejwe izindi ngero nka macandwe ninkari nibindi.

IBIKORWA BIKORWA

RINGARUKA
1.Hansen JH, n'abandi.HAMA Kwivanga kwa Murine Monoclonal Antibody ishingiye kuri Immunoassays [J] .J ya Clin Immunoassay, 1993,16: 294-299.
2.Levinson S.

Urufunguzo rwibimenyetso byakoreshejwe:

	Mubikoresho byubuvuzi bya Vitro
	Uruganda
	Ubike kuri 2-30 ℃
	Itariki izarangiriraho
	Ntugakoreshe
	ICYITONDERWA
	Baza Amabwiriza yo Gukoresha

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