Gusuzuma ibikoresho bya Isoenzyme Mb bya Creat Kinase(Fluorescence Umungororomatografia Assay)
Kuberako muri vitro ikoresha gusa
Nyamuneka soma iyi paki witonze mbere yo gukoresha kandi ukurikize neza amabwiriza. Kwizerwa kw'ibisubizo Ibisubizo ntibishobora kwizerwa niba hari gutandukana mumabwiriza muriyi paki.

Gukoresha
Gusuzuma Gusuzuma Isoenzyme Mb y'ibiremwa (Fluorescence Imnase (Fluorescence Imnase Amafaranga). Icyitegererezo cyose kigomba gushimangirwa nubundi buryo. Iki kizamini gigenewe gukoreshwa numwuga gusa.

Incamake
Urwego rwa CK-MB rwiyongereyeho amasaha 4-6 nyuma yamasaha ya Myocardial yabaye, amwegera amasaha 18-24, asubira muminsi 2-3 nyuma yiminsi 2-3. CK-MB Kurekura Kurekura nyuma ya Hemoglobibibing.ck-MB nicyo kimenyetso gakondo cyo gufata Myocardial

Ihame ry'uburyo
Ingano y'ibikoresho by'ikizamini ifatanye na antibody mu karere k'ikizamini n'ihene anti rubg antibod mu karere kagenzura. Amaduka yatowe na fluorescence yanditseho anti ck-mb antibody ninkwavu igg mbere. Iyo ugerageza icyitegererezo cyiza, CK-Mb Antigen muri sample ihuza na fluorescence yanditseho anti ck-mb antibody, kandi igace invance. Mubikorwa bya Imyumechromatography, urujya n'uruza rugana ku mpapuro zinjira, igihe bigoye kurenga akarere k'ikizamini, ihujwe na anti-mb igenamigambi rishya. Urwego rwa CK-MB rufitanye isano neza nikimenyetso cya fluorescence, kandi kwibanda kuri CK-MB murugero birashobora kumenyekana na fluorescence imnungosssas.

Reages nibikoresho byatanzwe

Ibikoresho 25t:
Ikarita y'Ibizamini Umuntu ku giti cye Yakozwe na Desiccant 25t
Icyitegererezo cya dilueds 25t
Ipaki Yinjije 1

Ibikoresho birakenewe ariko ntibitangwa
Icyitegererezo cyo gukusanya ibintu, igihe

Icyitegererezo cyo gukusanya no kubika
1.Icyitegererezo cyageragejwe gishobora kuba, Heparin anticogulant Plasma cyangwa EDTA Anticogulant Plasma.
2.Ubuhanga busanzwe bwo gukusanya icyitegererezo. Serum cyangwa Plasma Icyitegererezo gishobora gukomeza gukonjesha saa mbiri-8 ℃ ku minsi 7 na konko hepfo -15 ° C mumezi 6.
3.Icyitegererezo cyose irinde guhagarika inzinguzingo.

Uburyo bwo Gukwirakwiza
Nyamuneka soma igitabo cyigikoresho cyigikoresho na paki shyiramo mbere yo kwipimisha.
1.Ubundi ku ruhande reagents zose hamwe ningero kubushyuhe bwicyumba.
.
3.scan code yo gutesha agaciro kugirango yemeze ikintu cyibizamini.
4. Fata ikarita yikizamini kuva mu gikapu.
5.Mugukanda ikarita yikizamini mubibanza byamakarita, scan code ya QR, hanyuma umenye ikintu cyibizamini.
5.Add 40μl serumu cyangwa icyitegererezo cya plasma kuri sample dilunt, hanyuma uvange neza ..
6.Add 8μl icyitegererezo igisubizo kuri sample iriba ikarita.
7.Kugabanya buto "Ikizamini gisanzwe", nyuma yiminota 15, igikoresho kizahita kimenya ikarita yikizamini, irashobora gusoma ibisubizo bivuye mu kwerekana igikoresho, hanyuma wandike / andika ibisubizo byikizamini.
8.Buringaniza kunyigisho z'abacunganzida (Wiz-A101).

Indangagaciro ziteganijwe
Ck-mb <5ng / ml
Birasabwa ko buri laboratoire ishyiraho urwego rusanzwe rugereranya abarwayi bayo.

Ibisubizo n'ibisubizo
.Amakuru yavuzwe haruguru nigisubizo cyikizamini cya CK-MB, kandi birasabwa ko buri laboratoire igomba gushyiraho urwego rwibanze rwa CK-MB-MB-MB-MB-MB rukwiranye nabaturage muri kano karere. Ibisubizo byavuzwe haruguru nibyo bivugwa gusa.
.Ibisubizo byubu buryo bikoreshwa gusa kubijyanye ningingo zerekanwe muri ubu buryo, kandi ntagereranywa nubundi buryo.
.Ibintu birashobora kandi gutera amakosa kubisubizo, harimo impamvu za tekiniki, amakosa yimikorere nibindi bintu byintangarugero.

Kubika no gutuza
1.Ibikoresho bifite amezi 18 yuzuye - ubuzima kuva gukora. Bika ibikoresho bidakoreshwa kuri 2-30 ° C. Ntugahagarike. Ntukoreshe kurenza itariki izarangiriraho.
2.Ntukingure umufuka ufunze kugeza witeguye gukora ikizamini, kandi ikizamini cyo gukoresha kimwe gisabwa gukoreshwa (ubushyuhe 2-35 bishoboka.
3.Bisoma Diluennt ikoreshwa ako kanya nyuma yo gufungura.

Umuburo n'inyungu
.Ibikoresho bigomba gushyirwaho ikimenyetso no gukingirwa ubushuhe.
.Ibikoresho byiza bigenwa nubundi buryo.
.Ibigereranyo bizafatwa nkuwanduye.
.Ntukoreshe reagent yarangiye.
.Ntabwo uhanagura reagent mubikoresho hamwe na love hatandukanye oya ..
.Do ntukoreshe amakarita y'ibizamini hamwe nibikoresho byose bitashoboka.
.

LKwigana
.Kuvuga hamwe nigipimo icyo aricyo cyose gikoresha imbeba antibolies, ibishoboka bibaho kubavanga numuntu arwanya imbeba (Hama) murugero. Ingendo zituruka ku barwayi babonye imyiteguro ya antibodis ya monoclonal yo kwisuzumisha cyangwa kuvura bishobora kuba birimo hama. Ibyingenzi birashobora gutera ibisubizo byiza cyangwa ibinyoma.
.Ibisubizo byibizamini bigamije kuvugwa gusa, ntibigomba kuba ishingiro ryonyine ryo gusuzuma amavuriro no kuvurwa, Amateka y'ubuvuzi, mu yandi mateka, igisubizo cyo kuvura nandi makuru .
.Ibi bikoreshwa gusa kubizamini bya Serum nibizamini bya Plasma. Ntabwo ishobora kubona ibisubizo nyabyo iyo bikoreshwa mubindi byitegererezo nkamacandwe ninkari n'ikibi nibindi.

Ibiranga imikorere

RIbisobanuro
.
2.Levinson S. Imiterere ya antibodique ninshingano muri Impungo zivanze [j] .J wa Clin Imyungesay, 1992: 108-114.

Urufunguzo rwibimenyetso byakoreshejwe:

	Mugikoresho cyo kwivuza kwa vitro
	Uruganda
	Ububiko kuri 2-30 ℃
	Itariki yo kurangiriraho
	Ntukoreshe
	Kwitondera
	Ngera inama amabwiriza yo gukoresha

Xiamen wiz biotech co., Ltd
Aderesi: Igorofa 3-4, No.16 Amahugurwa yubuvuzi, Amahugurwa yubuvuzi, 2030 Wengjiao Umuhanda Wiburengerazuba, Akarere ka Haicang, 361026, Xiamen, Abapayiniya
Tel: + 86-592-6808278
Fax: + 86-592-6808279