Igikoresho cyo gusuzumahypertensitive C-proteine

(fluorescence immunochromatographic assay)

Kuri vitro kwisuzumisha koresha gusa

Nyamuneka soma iyi paki shyiramo witonze mbere yo gukoresha kandi ukurikize neza amabwiriza. Ibisubizo byizewe ntibishobora kwemezwa niba hari gutandukana kwamabwiriza muriyi paki yinjizamo.

GUKORESHA

Igikoresho cyo kwisuzumisha kuri hypertensitive C-reaction proteine ​​(fluorescence immunochromatographic assay) ni fluorescence immunochromatographic assay kugirango igaragaze umubare wa poroteyine C-reaction (CRP) muri serumu yumuntu / plasma / Amaraso yose. Nibimenyetso bidasanzwe byerekana umuriro. Icyitegererezo cyiza cyose kigomba kwemezwa nubundi buryo. Iki kizamini kigenewe ubuvuzi bwumwuga gusa.

INCAMAKE

C-reaction proteine ​​ni proteine ​​ikaze ikorwa na lymphokine itera umwijima na selile epithelia. Ibaho muri serumu yumuntu, cerebrospinal fluid, pleural and abdominal fluid, nibindi, kandi ni igice cyuburyo budasanzwe bwo kwirinda indwara. 6-8h nyuma yo kwibasirwa na bagiteri, CRP yatangiye kwiyongera, 24-48h igera ku mpinga, kandi agaciro k'impinga gashobora kugera ku magana asanzwe. Nyuma yo kurandura ubwandu, CRP yagabanutse cyane hanyuma isubira mubisanzwe mugihe cyicyumweru kimwe. Nyamara, CRP ntabwo yiyongera cyane mugihe cyanduye virusi, itanga umusingi wo kumenya ubwoko bwindwara hakiri kare, kandi nigikoresho cyo kumenya virusi cyangwa bagiteri.

IHame RY'UBURYO

Ibice by'igikoresho cyo kwipimisha bisizwe na antibody anti-CRP mu karere k'ibizamini hamwe na ihene irwanya urukwavu IgG antibody ku karere kayobora. Lable pad yashizwemo na fluorescence yanditseho antibody ya CRP ninkwavu IgG mbere. Iyo ugerageza icyitegererezo cyiza, antigen ya CRP murugero ikomatanya na fluorescence yanditseho antibody ya CRP, hanyuma igakora imvange yumubiri. Mubikorwa bya immunochromatografiya, urujya n'uruza rugana mu cyerekezo cyimpapuro zinjira, iyo complexe yatsinze akarere k’ibizamini, yahujwe na anti-CRP coating antibody, ikora urwego rushya. Urwego rwa CRP rufitanye isano neza na signal ya fluorescence, kandi kwibumbira hamwe kwa CRP murugero birashobora gutahurwa na fluorescence immunoassay assay.