Kuberako muri vitro ikoresha gusa

Nyamuneka soma iyi paki witonze mbere yo gukoresha kandi ukurikize neza amabwiriza. Kwizerwa kw'ibisubizo Ibisubizo ntibishobora kwizerwa niba hari gutandukana mumabwiriza muriyi paki.

Gukoresha

Dignostic kit kuri hepatite c virumbe antibods (fluorescence imyumechromatografiyaga uburyo. Iki kizamini kigenewe gukoreshwa numwuga gusa

1.Ubundi ku ruhande reagents zose hamwe ningero kubushyuhe bwicyumba.
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3.scan code yo gutesha agaciro kugirango yemeze ikintu cyibizamini.
4. Fata ikarita yikizamini kuva mu gikapu.
5.Mugukanda ikarita yikizamini mubibanza byamakarita, scan code ya QR, hanyuma umenye ikintu cyibizamini.
6.Add 20μl serumu cyangwa icyitegererezo cya plasma kuri sample dilunt, hanyuma uvange neza ..
7.Add 80μl icyitegererezo igisubizo kuri sample iriba ikarita.
8.Kwita "Ikizamini gisanzwe", nyuma yiminota 15, igikoresho kizahita kimenya ikarita yikizamini, irashobora gusoma ibisubizo bivuye mu kwerekana igikoresho, hanyuma wandike / andika ibisubizo byikizamini.
9.Gukoresha inyigisho z'abacunganzida (Wiz-A101).

Incamake

Hepatite C virusi (HCV) ni ibahasha, imyumvire myiza yanga RNA (9.5 KB) ya virusi yumuryango wa flavivilidae. Batandatu bakomeye ba Genotondes hamwe nuruhererekane rwa estypes ya HCV bamenyekanye. HCV yigunze mu 1989, HCV ubu yamenyekanye nkimpamvu nyamukuru yo guterwa ifitanye isano natari-a, atari b hepatite. Indwara irangwa n'ifishi ikaze n'ubura. Abantu barenga 50% banduye batera imbere cyane, ubuzima bwangiza ibintu bidakunze kurangira hamwe na liver cirrhose na karcinomasi ya hepatollium. Kuva intangiriro mu 1990 yo gusuzuma ibipimo bya maraso, indwara yaya yanduye mu kwanduza kwakirwa yagabanutse cyane. Ubushakashatsi bwa Clinical bwerekana ko umubare munini wa HCV abantu banduye HCV guteza imbere antibodiyidi muri oroteine ​​zitari imiterere ya virusi. Kubwibyo, ibizamini birimo antigens mukarere ka NS5 cya Gerum Genome wongeyeho NS3 (C200), NS4 (C200) na Core (C22).

Ihame ry'uburyo

Ingano y'ibikoresho by'ibizamini ikongejwe na HCV Antigen mu karere k'ikizamini n'ihene anti inkwavu igg antibod mu karere kagenzura. Amaduka yatowe na Fluorescence yanditseho HCV Antigen na Urukwavu Igg mbere. Iyo ugerageza icyitegererezo cyiza, antibody ya HCV muri sample ihuza na fluorescence yanditseho hcv antigen, kandi igace invance. Mubikorwa bya Immunochromatography, urujya n'uruza rugana mu cyerekezo cy'impapuro zinjira, igihe bigoye ku nkombe za antigen HCV Antibody murugero irashobora kumenyekana na fluorescence imwungosasay

Reages nibikoresho byatanzwe

Ibikoresho 25t:
.Ikarita ikarita kugiti cye
.
.Gukoreshamo

Ibikoresho birakenewe ariko ntibitangwa
Icyitegererezo cyo gukusanya ibintu, igihe
Icyitegererezo cyo gukusanya no kubika
1.Icyitegererezo cyageragejwe gishobora kuba, Heparin anticogulant Plasma cyangwa EDTA Anticogulant Plasma.
2.Ubuhanga busanzwe bwo gukusanya icyitegererezo. Icyitegererezo cyangwa icyapa cya plasma kirashobora guhora gikonjesha saa mbiri-8 ℃ kumunsi 7 na conopreerwervation hepfo -15 ° C amezi 6
3.Icyitegererezo cyose irinde guhagarika inzinguzingo.

Uburyo bwo Gukwirakwiza
Nyamuneka soma igitabo cyigikoresho cyigikoresho na paki shyiramo mbere yo kwipimisha.
.Ibisubizo byibizamini bigamije kuvugwa gusa, ntibigomba kuba ishingiro ryonyine ryo gusuzuma amavuriro no kuvurwa, Amateka y'ubuvuzi, mu yandi mateka, igisubizo cyo kuvura nandi makuru .
.Ibi bikoreshwa gusa kubizamini bya Serum nibizamini bya Plasma. Ntabwo ishobora kubona ibisubizo nyabyo iyo bikoreshwa mubindi byitegererezo nkamacandwe ninkari n'ikibi nibindi.

Ibiranga imikorere

INGINGO
1.Korekanwa no guterwa hepatitis. Muri: moore sb, ed. Indwara zo guterwa no guterwa. Alington, VA. AM. Assoc. Amabanki yamaraso, pp. 53-38.
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3.Levinson S. Imiterere ya antibodique ya heterophilic nuruhare muri Impongano yo kwivanga [j] .J wa Clin Imyungesay, 1992: 108-114.
4.Umunsi HJ., Purcell Rh, Holland PV, et al. . Lancet I: 459-463.
5.Hako ql, Weiner AJ, hejuru ya LR, Kuo G, Houghton M. (1990) Ishuri rya Hepatite C. (1990) Imbere ya Hepatitis C. Br br med bull 46: 423-441.
6.Erivisi e, Perlmann P. (1971) Enzyme yahujwe immyunnosorbent ismus (Elisa): Ishuri riremewe rya Igg. Imburambe 8: 871-874.

Indangagaciro ziteganijwe

HCV-AB <0.02

Birasabwa ko buri laboratoire ishyiraho urwego rusanzwe rugereranya abarwayi bayo.

Ibisubizo n'ibisubizo

• Amakuru yavuzwe haruguru nigisubizo cya HCV-AB Ikizamini cya HCV-AB, kandi birasabwe ko buri laboratoire igomba gushyiraho indangagaciro zitandukanye za HCV-AB ikwiranye nabaturage muri kano karere. Ibisubizo byavuzwe haruguru nibyo bivugwa gusa.
• Ibisubizo byubu buryo birakoreshwa gusa kubijyanye ningingo zerekanwe muri ubu buryo, kandi nta buryo butaziguye nubundi buryo.
• Ibindi bintu birashobora kandi gutera amakosa kubisubizo, harimo impamvu za tekiniki, amakosa yimikorere nibindi bintu byintangarugero.

Kubika no gutuza

1. Ibikoresho ni amezi 18 yuzuye - ubuzima buva ku munsi wo gukora. Bika ibikoresho bidakoreshwa kuri 2-30 ° C. Ntugahagarike. Ntukoreshe kurenza itariki izarangiriraho.
2. Ntukingure umufuka ufunze kugeza witeguye gukora ikizamini, kandi ikizamini cyo gukoresha kimwe gisabwa gukoreshwa mu bidukikije bisabwa (ubushyuhe 2-35 ℃, ubushyuhe bwa 2-90% .
3. Icyitegererezo cya Didele gikoreshwa ako kanya nyuma yo gufungura.

Umuburo n'inyungu
.Ibikoresho bigomba gushyirwaho ikimenyetso no gukingirwa ubushuhe.
.Ibikoresho byiza bigenwa nubundi buryo.
.Ingero zose zigomba gufatwa nkibishobora guhungabana.
.Ntukoreshe reagent yarangiye.
.Ntugahanagura reagent mubikoresho hamwe na love hatandukanye oya ..
.Ntukoreshe amakarita y'ibizamini nibikoresho byose bitashoboka.
.Gukundana, urugero rukabije cyangwa ruto rushobora kuganisha kubisubizo.

LKwigana
.Kimwe nigipimo icyo ari cyo cyose gikoresha imbeba antibodies, ibishoboka bibaho kuba indabyo na antibodi yibembe (Hama) murugero. Ingendo zituruka ku barwayi babonye imyiteguro ya antibodis ya monoclonal yo kwisuzumisha cyangwa kuvura bishobora kuba birimo hama. Ibyingenzi birashobora gutera ibisubizo byiza cyangwa ibinyoma.
Urufunguzo rwibimenyetso byakoreshejwe:

	Mugikoresho cyo kwivuza kwa vitro
	Uruganda
	Ububiko kuri 2-30 ℃
	Itariki yo kurangiriraho
	Ntukoreshe
	Kwitondera
	Ngera inama amabwiriza yo gukoresha