Igikoresho cyo gusuzumaCalprotectin(Fluorescence Immunochromatographic Assay)
Kuri vitro kwisuzumisha koresha gusa
Nyamuneka soma iyi paki shyiramo witonze mbere yo gukoresha kandi ukurikize neza amabwiriza. Ibisubizo byizewe ntibishobora kwemezwa niba hari gutandukana kwamabwiriza muriyi paki yinjizamo.

GUKORESHA
Igikoresho cyo gusuzumaCalprotectin. Iki kizamini kigenewe ubuvuzi bwumwuga gusa.

INCAMAKE
Cal ni heterodimer, igizwe na MRP 8 na MRP 14^[1]. Ibaho muri neutrophile cytoplasm kandi igaragarira kuri selile monon nuclear. Cal ni poroteyine ikaze, ifite icyiciro gihamye hafi icyumweru kimwe mumyanda yabantu, yiyemeje kuba ikimenyetso cyindwara yumura.^[2-3]. Igikoresho ni ikizamini cyoroshye, kigaragara cyerekana Cal mu myanda yabantu, gifite sensibilité yo hejuru kandi yihariye. Ikizamini gishingiye cyane cyane kuri antibodies ebyiri sandwich reaction hamwe na fluorescence immunochromatographic assay tekinike yo gusesengura, ishobora gutanga ibisubizo muminota 15.

IHame RY'UBURYO
Igice gifite antibody ya anti-Cal coating mukarere ka test, ifatanye na membrane chromatografi mbere. Lable pad yashizwemo na fluorescence yanditseho antibody anti-Cal mbere. Mugihe cyo gupima icyitegererezo cyiza, Cal murugero irashobora kuvangwa na fluorescence yanditseho antibody anti-Cal, hanyuma igakora imvange yumubiri. Nkuko uruvange rwemerewe kwimuka kuruhande rwibizamini, uruganda rwa Cal conjugate rufatwa na antibody anti-Cal coating antibody kuri membrane hanyuma ikora complexe. Imbaraga za fluorescence zifitanye isano neza nibirimo Cal. Cal mubyitegererezo irashobora gutahurwa na fluorescence immunoassay isesengura.

REAGENTS N'IBIKORWA BYUZUWE

25T ibice：
Ikarita yikizamini kugiti cyayo ifatanye na desiccant 25T
Icyitegererezo cya 25T
Shyiramo paki 1

IBIKORWA BISABWA ARIKO NTIBITANZWE
Icyitegererezo cyo gukusanya ibikoresho, igihe

GUKORANYA URUGERO N'UBubiko
1.Koresha ikintu gisukuye kugirango ukusanyirize hamwe umwanda mushya, hanyuma ugerageze ako kanya. Niba bidashobora kugeragezwa ako kanya, nyamuneka ubike kuri 2-8 ° C muminsi 3 cyangwa munsi ya -15 ° C mumezi 6.
2.Kuramo inkoni y'icyitegererezo, winjizwe mucyitegererezo cy'umwanda, subiramo ibikorwa inshuro 3, fata ibice bitandukanye by'icyitegererezo cy'umwanda buri gihe, hanyuma usubize inkoni y'icyitegererezo inyuma, ucye neza kandi uzunguze neza, Cyangwa ukoresheje inkoni y'icyitegererezo yatowe icyitegererezo cya 50mg cyicyitegererezo, hanyuma ugashyiramo umwanda wicyitegererezo urimo urugero rwicyitegererezo, hanyuma ugasunika neza.
3.Koresha icyitegererezo cya pipette ikoreshwa fata icyitegererezo cyumwanda kumurwayi wimpiswi, hanyuma wongereho ibitonyanga 3 (hafi 100µL) mumiyoboro ya fecal sampling hanyuma uzunguze neza.

Inyandiko:
1. Irinde kuzenguruka-gukonjesha.
2.Kora icyitegererezo kubushyuhe bwicyumba mbere yo gukoresha.

GUKORA UBURYO

Nyamuneka soma igitabo gikoreshwa nigikoresho cyo gushyiramo mbere yo kugerageza.
1. Shira ku ruhande reagent zose hamwe nicyitegererezo kubushyuhe bwicyumba.
2.Fungura Portable Immune Analyser (WIZ-A101), andika ijambo ryibanga rya konte ukurikije uburyo bwo gukora igikoresho, hanyuma winjire muburyo bwo gutahura.
3.Shobora kode ya dentification kugirango wemeze ikintu cyizamini.
4.Kuramo ikarita yikizamini mu mufuka wa file.
5. Shyiramo ikarita yikizamini ahantu h'ikarita, suzuma kode ya QR, hanyuma umenye ikintu cyizamini.
6.Kura ingofero muri sample ya sample hanyuma ujugunye ibitonyanga bibiri byambere byongeweho icyitegererezo, ongeramo ibitonyanga 3 (hafi 100uL) nta bubble buvanze sample verticaly hanyuma buhoro buhoro mubyitegererezo byikarita yatanzwe.
7.Kanda buto ya "test test", nyuma yiminota 15, igikoresho kizahita kimenya ikarita yikizamini, kirashobora gusoma ibisubizo bivuye kuri ecran yerekana igikoresho, hanyuma wandike / wandike ibisubizo byikizamini.
8.Reba amabwiriza ya Portable Immune Analyser (WIZ-A101).

AGACIRO KATEGANYIJWE
Cal <60μg / g
Birasabwa ko buri laboratoire ishyiraho urwego rwayo rusanzwe ruhagarariye abaturage barwaye.

IBISUBIZO BY'IKIZAMINI NO GUSOBANURA
1. Cal muri sample irenze 60μg / g, kandi igomba kwirinda ihinduka ryimiterere ya physiologique. Ibisubizo rwose ntibisanzwe kandi bigomba gusuzumwa nibimenyetso byubuvuzi.
2.Ibisubizo byubu buryo birakoreshwa gusa murwego rwerekanwe rwashyizweho muri ubu buryo, kandi ntaho bihuriye nubundi buryo.
3.Ibindi bintu birashobora kandi gutera amakosa mubisubizo byo gutahura, harimo impamvu za tekiniki, amakosa yimikorere nibindi bintu byintangarugero.

Ububiko N'UBUHAMYA
1.Ibikoresho ni amezi 18 yo kubaho-uhereye igihe byakorewe. Bika ibikoresho bidakoreshwa kuri 2-30 ° C. NTUBUNTU. Ntugakoreshe kurenza itariki izarangiriraho.
2.Ntukingure umufuka ufunze kugeza igihe witeguye gukora ikizamini, kandi ikizamini kimwe cyo gukoreshwa gisabwa gukoreshwa mubisabwa (ubushyuhe 2-35 ℃, ubuhehere 40-90%) muminota 60 byihuse. bishoboka.
3.Urugero rwa diluent rukoreshwa ako kanya nyuma yo gufungura.

UMUBURO N'UBWITONDERWA
.Ibikoresho bigomba gufungwa kandi bikarindwa ubushuhe.
.Ingero zose nziza zemezwa nubundi buryo.
.Ingero zose zifatwa nkibishobora guhumanya.
.Ntugakoreshe reagent yarangiye.
.NtUGASIMBURE reagent mubikoresho bifite ubufindo butandukanye Oya ..
.NtUGASUBIZE gukoresha amakarita yikizamini nibikoresho byose bikoreshwa.
.Gukoresha nabi, urugero rwinshi cyangwa ruto rushobora kuganisha ku gutandukana.

LIMITATION
.Nkuko hamwe nubushakashatsi ubwo aribwo bwose bukoresha antibodi yimbeba, birashoboka ko habaho kwivanga na antibodiyite zabantu barwanya imbeba (HAMA) murugero. Ingero z'abarwayi bahawe imyiteguro ya antibodiyite ya monoclonal yo gusuzuma cyangwa kuvura irashobora kuba irimo HAMA. Ingero nkizo zishobora gutera ibinyoma byiza cyangwa ibinyoma bibi.
.Ibisubizo by'ibizamini ni ibyerekeye ivuriro gusa, ntibigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura kwa muganga, imicungire y’amavuriro y’abarwayi igomba kwitabwaho byimazeyo hamwe n’ibimenyetso byayo, amateka y’ubuvuzi, ibindi bizamini bya laboratoire, igisubizo cy’ubuvuzi, epidemiologiya nandi makuru .
.Iyi reagent ikoreshwa gusa mugupima fecal. Ntishobora kubona ibisubizo nyabyo mugihe ikoreshejwe izindi ngero nka macandwe ninkari nibindi.

IBIKORWA BIKORWA

INGINGO
1.Li, G. & Y.L.Li.Isano iri hagati ya calcium n'indwara zo kwa muganga [J] .Ikinyamakuru cya Medicine Practical Medicine, 2007,23 (15)
2.Han, W,
3.Wang, ZH, Guo, HB, n'abandi. Ubushakashatsi ku isano iri hagati ya fecal calamine n'indwara y'amara [J]. Ubumenyi bwa siyansi nubuhanga, 2010-03,10 (8)

Urufunguzo rwibimenyetso byakoreshejwe:

	Mubikoresho byubuvuzi bya Vitro
	Uruganda
	Ubike kuri 2-30 ℃
	Itariki izarangiriraho
	Ntugakoreshe
	ICYITONDERWA
	Baza Amabwiriza yo Gukoresha

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