GusuzumaCalprotectin(Fluorescence Umungororomatografia Assay)
Kuberako muri vitro ikoresha gusa
Nyamuneka soma iyi paki witonze mbere yo gukoresha kandi ukurikize neza amabwiriza. Kwizerwa kw'ibisubizo Ibisubizo ntibishobora kwizerwa niba hari gutandukana mumabwiriza muriyi paki.

Gukoresha
Gusuzuma ibikoresho bya Calprotectin (Cal) birakwiriye kugena umubare wa Fluorescence Imyumbati Iki kizamini gigenewe gukoreshwa numwuga gusa.

Incamake
Cal ni umukunzi, igizwe na MRP 8 na MRP 14^[1]. Ihari muri Netrophils cytoplasm kandi igaragarira kuri kasheli ya monnonuclear. Cal ni protese ya ikaze, ifite icyiciro gihamye kizengurutse icyumweru kimwe mumukunzi wabantu, yiyemeje kuba intandaro yindwara yatewe na fumber^[2-3]. Ikizamini cyoroshye, gifatika gifatika kivuga ko cashe mumubiri wabantu, gifite ubushishozi bwo hejuru no kwiyumvisha neza. Ikizamini gishingiye ku buryo burenze urugero rwa antibodies ebyiri sandwich ihame na fluorescence umushunochromatograppic tekinike, ishobora gutanga ibisubizo mu minota 15.

Ihame ry'uburyo
Umurongo ufite Anti-yo Gutwika Antibody mu karere k'ikizamini, bihambiriye kuri membrane Chromatography mbere. Inted Pad ikomatanwa na fluorescence yanditseho anti-cal antibody mbere. Iyo ugerageza icyitegererezo cyiza, inyana murugero rushobora kuvangwa na fluorescence yanditseho anti-cal antibody, kandi igace invance. Nkuko imvange yemerewe kwimuka kumurongo wikizamini, igipimo cya cal conjugate cyafashwe numubongo wa anti-inyana kuri membrane no gukora bigoye. Ubukana bwa fluorescence bufitanye isano neza nibirimo. Inyana iri murugero rushobora kumenyekana na fluorescence isesengura.

Reages nibikoresho byatanzwe

Ibikoresho 25t:
Ikarita y'Ibizamini Umuntu ku giti cye Yakozwe na Desiccant 25t
Icyitegererezo cya dilueds 25t
Ipaki Yinjije 1

Ibikoresho birakenewe ariko ntibitangwa
Icyitegererezo cyo gukusanya ibintu, igihe

Icyitegererezo cyo gukusanya no kubika
1. Koresha ibintu bitagereranywa kugirango ukusanye umwambe mwiza icyitegererezo, kandi ugabanywa ako kanya. Niba bidashobora kugeragezwa ako kanya, nyamuneka ubike kuri 2-8 ° C muminsi 3 cyangwa munsi -15 ° C amezi 6.
2.Komera inkono yipimbano, winjizwe mumyambarire icyitegererezo, subiramo ibikorwa inshuro 3, fata ibice bitandukanye bya FAEPLE NYUMA, hanyuma ushireho neza, cyangwa ukoreshe neza, cyangwa ukoresheje inkoni yatoranijwe Hafi ya Feeces Samece, hanyuma ushireho umwambaro icyitegererezo kirimo icyitegererezo, kandi gikubise neza.
3.Usemyi ya pipette ya feipette ifata umwambaro icyitegererezo cyo muri Diarrhea, hanyuma ongeraho ibitonyanga 3μl) kuri tube ya fecal no kunyeganyega neza.

Icyitonderwa:
1.Ibike.
2.Thaw ingero kubushyuhe bwicyumba mbere yo gukoresha.

Uburyo bwo Gukwirakwiza

Nyamuneka soma igitabo cyigikoresho cyigikoresho na paki shyiramo mbere yo kwipimisha.
1.Ubundi ku ruhande reagents zose hamwe ningero kubushyuhe bwicyumba.
.
3.scan code yo gutesha agaciro kugirango yemeze ikintu cyibizamini.
4. Fata ikarita yikizamini kuva mu gikapu.
5.Mugukanda ikarita yikizamini mubibanza byamakarita, scan code ya QR, hanyuma umenye ikintu cyibizamini.
6.Remera ingofero yo hejuru hanyuma ujugunye ibitonyanga bibiri byambere bivanga icyitegererezo, ongeramo ibitonyanga 3 (hafi 100) nta busambanyi bwanditseho umusaruro.
7.Kugabanya buto "Ikizamini gisanzwe", nyuma yiminota 15, igikoresho kizahita kimenya ikarita yikizamini, irashobora gusoma ibisubizo bivuye mu kwerekana igikoresho, hanyuma wandike / andika ibisubizo byikizamini.
8.Buringaniza kunyigisho z'abacunganzida (Wiz-A101).

Indangagaciro ziteganijwe
Cal <60μ / g
Birasabwa ko buri laboratoire ishyiraho urwego rusanzwe rugereranya abarwayi bayo.

Ibisubizo n'ibisubizo
1.Izaga iri murugero nibyinshi muri 60μg / g, kandi bigomba guhamya impinduka za physiologiya. Ibisubizo rwose bidasanzwe kandi bigomba gusuzumwa nibimenyetso byubuvuzi.
2.Ibisubizo by'ubu buryo gikoreshwa gusa ku ruhame ngenderwaho cyashyizweho muri ubu buryo, kandi nta buryo butaziguye n'ubundi buryo.
3.Ibintu birashobora kandi gutera amakosa kubisubizo, harimo impamvu za tekiniki, amakosa yimikorere nibindi bintu byintangarugero.

Kubika no gutuza
1.Ibikoresho bifite amezi 18 yuzuye - ubuzima kuva gukora. Bika ibikoresho bidakoreshwa kuri 2-30 ° C. Ntugahagarike. Ntukoreshe kurenza itariki izarangiriraho.
2.Ntukingure umufuka ufunze kugeza witeguye gukora ikizamini, kandi ikizamini cyo gukoresha kimwe gisabwa gukoreshwa (ubushyuhe 2-35 bishoboka.
3.Bisoma Diluennt ikoreshwa ako kanya nyuma yo gufungura.

Umuburo n'inyungu
.Ibikoresho bigomba gushyirwaho ikimenyetso no gukingirwa ubushuhe.
.Ibikoresho byiza bigenwa nubundi buryo.
.Ibigereranyo bizafatwa nkuwanduye.
.Ntukoreshe reagent yarangiye.
.Ntabwo uhanagura reagent mubikoresho hamwe na love hatandukanye oya ..
.Do ntukoreshe amakarita y'ibizamini hamwe nibikoresho byose bitashoboka.
.

Imbogamizi
.Kuvuga hamwe nigipimo icyo aricyo cyose gikoresha imbeba antibolies, ibishoboka bibaho kubavanga numuntu arwanya imbeba (Hama) murugero. Ingendo zituruka ku barwayi babonye imyiteguro ya antibodis ya monoclonal yo kwisuzumisha cyangwa kuvura bishobora kuba birimo hama. Ibyingenzi birashobora gutera ibisubizo byiza cyangwa ibinyoma.
.Ibisubizo byibizamini bigamije kuvugwa gusa, ntibigomba kuba ishingiro ryonyine ryo gusuzuma amavuriro no kuvurwa, Amateka y'ubuvuzi, mu yandi mateka, igisubizo cyo kuvura nandi makuru .
.Ibi bikoreshwa gusa kubizamini bya fecal. Ntabwo ishobora kubona ibisubizo nyabyo iyo bikoreshwa mubindi byitegererezo nkamacandwe ninkari n'ikibi nibindi.

Ibiranga imikorere

INGINGO
.
2.Hani, W., Xu, JM, et al.Inyigisho za Calinine ya Calamine na Lactoferrin mu barwayi bafite ibisekuruza by'ibiseri,. Ikinyamakuru Gistroenterology, 2010.15 (4)
3.Wang, zh, Guo, HB, et al.Ubushakashatsi ku mibanire iri hagati ya kamere ya fecal n'indwara y'umuriro [j]. Ikoranabuhanga rya siyanse n'Ubuhanga, 2010-03,10 (8)

Urufunguzo rwibimenyetso byakoreshejwe:

	Mugikoresho cyo kwivuza kwa vitro
	Uruganda
	Ububiko kuri 2-30 ℃
	Itariki yo kurangiriraho
	Ntukoreshe
	Kwitondera
	Ngera inama amabwiriza yo gukoresha

Xiamen wiz biotech co., Ltd
Aderesi: Igorofa 3-4, No.16 Amahugurwa yubuvuzi, Amahugurwa yubuvuzi, 2030 Wengjiao Umuhanda Wiburengerazuba, Akarere ka Haicang, 361026, Xiamen, Abapayiniya
Tel: + 86-592-6808278
Fax: + 86-592-6808279