Igikoresho cyo gusuzumahypertensitive C-proteine

(fluorescence immunochromatographic assay)

Kuri vitro kwisuzumisha koresha gusa

Nyamuneka soma iyi paki shyiramo witonze mbere yo gukoresha kandi ukurikize neza amabwiriza. Ibisubizo byizewe ntibishobora kwemezwa niba hari gutandukana kwamabwiriza muriyi paki yinjizamo.

GUKORESHA

Igikoresho cyo kwisuzumisha kuri hypertensitive C-reaction proteine ​​(fluorescence immunochromatographic assay) ni fluorescence immunochromatographic assay kugirango igaragaze umubare wa poroteyine C-reaction (CRP) muri serumu yumuntu / plasma / Amaraso yose. Nibimenyetso bidasanzwe byerekana umuriro. Icyitegererezo cyiza cyose kigomba kwemezwa nubundi buryo. Iki kizamini kigenewe ubuvuzi bwumwuga gusa.

INCAMAKE

C-reaction proteine ​​ni proteine ​​ikaze ikorwa na lymphokine itera umwijima na selile epithelia. Ibaho muri serumu yumuntu, cerebrospinal fluid, pleural and abdominal fluid, nibindi, kandi ni igice cyuburyo budasanzwe bwo kwirinda indwara. 6-8h nyuma yo kwibasirwa na bagiteri, CRP yatangiye kwiyongera, 24-48h igera ku mpinga, kandi agaciro k'impinga gashobora kugera ku magana asanzwe. Nyuma yo kurandura ubwandu, CRP yagabanutse cyane hanyuma isubira mubisanzwe mugihe cyicyumweru kimwe. Nyamara, CRP ntabwo yiyongera cyane mugihe cyanduye virusi, itanga umusingi wo kumenya ubwoko bwindwara hakiri kare, kandi nigikoresho cyo kumenya virusi cyangwa bagiteri.

IHame RY'UBURYO

Ibice by'igikoresho cyo kwipimisha bisizwe na antibody anti-CRP mu karere k'ibizamini hamwe na ihene irwanya urukwavu IgG antibody ku karere kayobora. Lable pad yashizwemo na fluorescence yanditseho antibody ya CRP ninkwavu IgG mbere. Iyo ugerageza icyitegererezo cyiza, antigen ya CRP murugero ikomatanya na fluorescence yanditseho antibody ya CRP, hanyuma igakora imvange yumubiri. Mubikorwa bya immunochromatografiya, urujya n'uruza rugana mu cyerekezo cyimpapuro zinjira, iyo complexe yatsinze akarere k’ibizamini, yahujwe na anti-CRP coating antibody, ikora urwego rushya. Urwego rwa CRP rufitanye isano neza na signal ya fluorescence, kandi kwibumbira hamwe kwa CRP murugero birashobora gutahurwa na fluorescence immunoassay assay.

REAGENTS N'IBIKORWA BYUZUWE

25T ibice：

Ikarita yikizamini kugiti cyayo ifatanye na desiccant 25T

Icyitegererezo cya 25T

Shyiramo paki 1

IBIKORWA BISABWA ARIKO NTIBITANZWE

Icyitegererezo cyo gukusanya ibikoresho, igihe

GUKORANYA URUGERO N'UBubiko

1. Ingero zapimwe zishobora kuba serumu, heparin anticoagulant plasma cyangwa plasma ya EDTA anticoagulant.
2. Ukurikije tekinike isanzwe ikusanya icyitegererezo. Icyitegererezo cya serumu cyangwa plasma birashobora kubikwa muri firigo kuri 2-8 ℃ muminsi 7 hamwe no kubika munsi -15 ° C mumezi 6. Amaraso yose arashobora kubikwa muri firigo kuri 2-8 ℃ muminsi 3
3. Icyitegererezo cyose wirinde gukonjesha.