Gusuzumahypersensitive c-reactive poroteyine

(Fluorescence Umungororomatografia Assay)

Kuberako muri vitro ikoresha gusa

Nyamuneka soma iyi paki witonze mbere yo gukoresha kandi ukurikize neza amabwiriza. Kwizerwa kw'ibisubizo Ibisubizo ntibishobora kwizerwa niba hari gutandukana mumabwiriza muriyi paki.

Gukoresha

Gusuzuma ibikoresho bya C-Reacysensitive (Fluorescence Imyunochromatograchic assuy) ni fluorescence imyume Nicyo kimenyetso kidasanzwe cyo gutwika. Icyitegererezo cyose kigomba gushimangirwa nubundi buryo. Iki kizamini gigenewe gukoreshwa numwuga gusa.

Incamake

Poroteyine ya C-Reactique ni proteine ​​ikaze yakozwe na lymphokine kubyutsa umwijima na epithelial selile. Irahari muri serumu y'abantu, amazi yo mu bwami, amazi menshi kandi yo munda, n'ibindi, kandi akaba ari igice cy'ubuhungiro. 6-8h nyuma yo kubaho kwa kwandura bagiteri, CRP yatangiye kwiyongera, 24-48h yageze ku mpinga, kandi agaciro ka fatizo karashobora kugera inshuro amajana y'ibisanzwe. Nyuma yo kurandura infection, CRP yataye cyane asubira mubisanzwe mugihe cyicyumweru kimwe. Ariko, CRP ntabwo yiyongera cyane kubijyanye no kwandura virusi, itanga ishingiro ryo kumenya ubwoko bwindwara zanduye, kandi ni igikoresho cyo kumenya indwara za virusi cyangwa bagiteri.

Ihame ry'uburyo

Igikoresho cyikizamini gifatanye hamwe na anti crp antibody mukarere kakiza kandi ihene yo kurwanya urukwavu igg antibod antibodri. Amadozi yashizwemo na fluorescence yanditseho anti crp antibody ninkwavu igg mbere. Iyo ugerageza icyitegererezo cyiza, antigen ya CRP in dople ihuza na fluorescence yanditseho anti crp antibody, hanyuma ukore imvange. Mubikorwa bya Immunochromatography, urujya n'uruza rugana ku mpapuro zinjira, igihe bigoye byanyuze mu karere k'ikizamini, ihujwe na anti crp crp crp crp crp crp crp crp crp crp crp crp crp crp crp crp crp crp Urwego rwa CRP rufitanye isano neza nikimenyetso cya fluorescence, kandi kwibanda kuri crp murugero rushobora kumenyekana na fluorescence imnungosssay.

Reages nibikoresho byatanzwe

Ibikoresho 25t:

Ikarita y'Ibizamini Umuntu ku giti cye Yakozwe na Desiccant 25t

Icyitegererezo cya dilueds 25t

Ipaki Yinjije 1

Ibikoresho birakenewe ariko ntibitangwa

Icyitegererezo cyo gukusanya ibintu, igihe

Icyitegererezo cyo gukusanya no kubika

1. Urugero rwageragejwe rushobora kuba, Heparin anticogulant Plasma cyangwa Edta anticogulant Plasma.
2. Nk'uko tekinike isanzwe ikusanya icyitegererezo. Serum cyangwa Plasma Icyitegererezo gishobora gukomeza gukonjesha saa mbiri-8 ℃ ku minsi 7 na konko hepfo -15 ° C mumezi 6. Icyitegererezo cyamaraso cyose kirashobora guhora gikonjesha saa munani-8 ℃ iminsi 3
3. Icyitegererezo cyose kirinde guhagarika inzinguzingo.