Igikoresho cyo gusuzuma Antibody kuri Helicobacter Pylori (Fluorescence Immunochromatographic Assay)

ibisobanuro bigufi:


  • Igihe cyo kwipimisha:Iminota 10-15
  • Igihe cyemewe:Amezi 24
  • Ukuri:Kurenga 99%
  • Ibisobanuro:1/25 ikizamini / agasanduku
  • Ubushyuhe bwo kubika:2 ℃ -30 ℃
  • Ibicuruzwa birambuye

    Ibicuruzwa

    Igikoresho cyo Gusuzuma Antibody kuri Helicobacter Pylori(Fluorescence Immunochromatographic Assay)
    Kuri vitro kwisuzumisha koresha gusa

    Nyamuneka soma iyi paki shyiramo witonze mbere yo gukoresha kandi ukurikize neza amabwiriza. Ibisubizo byizewe ntibishobora kwemezwa niba hari gutandukana kwamabwiriza muriyi paki yinjizamo.

    UKORESHEJWE
    Igikoresho cyo gusuzuma Antibody kuri Helicobacter Pylori (Fluorescence Immunochromatographic Assay) ni fluorescence immunochromatographic assay yo kumenya umubare wa antibody ya HP muri serumu cyangwa plasma. ningirakamaro yingirakamaro yo kwisuzumisha agaciro kwandura gastric.Icyitegererezo cyiza cyose kigomba kwemezwa nubundi buryo. Iki kizamini kigenewe ubuvuzi bwumwuga gusa.

    INCAMAKE
    Indwara ya Gastric helicobacter pylori ifitanye isano rya bugufi na gastrite idakira, ibisebe byo mu gifu, adenocarcinoma gastric, mucosa gastrica ifitanye isano na lymphoma, Hp ylori yanduye hafi 90% muri gastrite, ibisebe byo mu gifu, ibisebe byo mu nda no ku barwayi ba kanseri yo mu gifu. Ninde wamenye h. pylori nkubwoko bwa mbere bwibintu bitera kanseri.ni ibintu bishobora gutera kanseri yo mu gifu.H.pylori gutahura bifite agaciro gakomeye mugupima h. kwandura pylori.

    IHame RY'UBURYO
    Ibice by'igikoresho cyo kwipimisha bisizwe hamwe na antigen ya HP mu karere k'ibizamini hamwe n'ihene irwanya urukwavu IgG antibody ku karere kayobora. Lable pad isizwe na fluorescence yanditseho HP antigen ninkwavu IgG mbere. Iyo ugerageza icyitegererezo cyiza, antibody ya HP murugero ihuza na fluorescence yanditseho HP antigen, hanyuma igakora imvange yubudahangarwa. Mubikorwa bya immunochromatografiya, urujya n'uruza rugana mu cyerekezo cyimpapuro zinjira, iyo complexe yatsinze akarere kizamini, ihuza na HP coating antigen, ikora urwego rushya.Urwego rwa HP-Ab rufitanye isano neza nibimenyetso bya fluorescence, hamwe no kwibanda kwa HP-Ab murugero irashobora gutahurwa na fluorescence immunoassay assay

    REAGENTS N'IBIKORWA BYUZUWE

    25T ibice
    Ikarita yikizamini kugiti cyayo ifatanye na desiccant 25T
    Icyitegererezo cya 25T
    Shyiramo paki 1

    IBIKORWA BISABWA ARIKO NTIBITANZWE
    Icyitegererezo cyo gukusanya ibikoresho, igihe

    GUKORANYA URUGERO N'UBubiko
    1.Ingero zapimwe zishobora kuba serumu, heparin anticoagulant plasma cyangwa plasma ya EDTA anticoagulant.

    2.Kurikije tekiniki zisanzwe zegeranya icyitegererezo. Icyitegererezo cya serumu cyangwa plasma birashobora kubikwa muri firigo kuri 2-8 ℃ muminsi 7 hamwe no kubika munsi -15 ° C mumezi 6.
    3.Icyitegererezo cyose wirinde gukonjesha.

    GUKORA UBURYO
    Nyamuneka soma igitabo gikoreshwa nigikoresho cyo gushyiramo mbere yo kugerageza.

    1. Shira ku ruhande reagent zose hamwe nicyitegererezo kubushyuhe bwicyumba.
    2.Fungura Portable Immune Analyser (WIZ-A101), andika ijambo ryibanga rya konte ukurikije uburyo bwo gukora igikoresho, hanyuma winjire muburyo bwo gutahura.
    3.Shobora kode ya dentification kugirango wemeze ikintu cyizamini.
    4.Kuramo ikarita yikizamini mu mufuka wa file.
    5. Shyiramo ikarita yikizamini ahantu h'ikarita, suzuma kode ya QR, hanyuma umenye ikintu cyizamini.
    6. Ongeramo 20μL serumu cyangwa plasma icyitegererezo kugirango utange urugero, hanyuma uvange neza ..
    7. Ongeraho 80μL igisubizo cyicyitegererezo kugirango utange neza ikarita.
    8.Kanda buto ya "ikizamini gisanzwe", nyuma yiminota 15, igikoresho kizahita kimenya ikarita yikizamini, irashobora gusoma ibisubizo bivuye kuri ecran yerekana igikoresho, hanyuma wandike / wandike ibisubizo byikizamini.
    9. Reba ku mabwiriza ya Portable Immune Analyser (WIZ-A101).

    AGACIRO KATEGANYIJWE
    HP-Ab <10

    Birasabwa ko buri laboratoire ishyiraho urwego rwayo rusanzwe ruhagarariye abaturage barwaye.

    IBISUBIZO BY'IKIZAMINI NO GUSOBANURA
    .Amakuru yavuzwe haruguru ni ibisubizo by'ikizamini cya HP-Ab, kandi hasabwa ko buri laboratoire igomba gushyiraho indangagaciro zitandukanye zo kumenya HP-Ab zibereye abaturage bo muri aka karere. Ibisubizo byavuzwe haruguru nibisobanuro gusa.

    .Ibisubizo byubu buryo birakoreshwa gusa murwego rwoherejwe rwashyizweho muri ubu buryo, kandi ntaho bihuriye nubundi buryo.
    .Ibindi bintu birashobora kandi gutera amakosa mubisubizo byo gutahura, harimo impamvu za tekiniki, amakosa yimikorere nibindi bintu byintangarugero.

    Ububiko N'UBUHAMYA
    1.Ibikoresho ni amezi 18 yo kubaho-uhereye igihe byakorewe. Bika ibikoresho bidakoreshwa kuri 2-30 ° C. NTUBUNTU. Ntugakoreshe kurenza itariki izarangiriraho.

    2.Ntukingure umufuka ufunze kugeza igihe witeguye gukora ikizamini, kandi ikizamini kimwe cyo gukoreshwa gisabwa gukoreshwa mubisabwa (ubushyuhe 2-35 ℃, ubuhehere 40-90%) muminota 60 byihuse. bishoboka.
    3.Urugero rwa diluent rukoreshwa ako kanya nyuma yo gufungura.

    UMUBURO N'UBWITONDERWA
    .Ibikoresho bigomba gufungwa kandi bikarindwa ubushuhe.

    .Ingero zose nziza zemezwa nubundi buryo.
    .Ingero zose zifatwa nkibishobora guhumanya.
    .Ntugakoreshe reagent yarangiye.
    .NtUGASIMBURE reagent mubikoresho bifite ubufindo butandukanye Oya ..
    .NtUGASUBIZE gukoresha amakarita yikizamini nibikoresho byose bikoreshwa.
    .Gukoresha nabi, urugero rwinshi cyangwa ruto rushobora kuganisha ku gutandukana.

    LKwigana
    .Nkuko hamwe nubushakashatsi ubwo aribwo bwose bukoresha antibodi yimbeba, birashoboka ko habaho kwivanga na antibodiyite zabantu barwanya imbeba (HAMA) murugero. Ingero z'abarwayi bahawe imyiteguro ya antibodiyite ya monoclonal yo gusuzuma cyangwa kuvura irashobora kuba irimo HAMA. Ingero nkizo zishobora gutera ibinyoma byiza cyangwa ibinyoma bibi.

    .Ibisubizo by'ibizamini ni ibyerekeye ivuriro gusa, ntibigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura kwa muganga, imicungire y’amavuriro y’abarwayi igomba kwitabwaho byimazeyo hamwe n’ibimenyetso byayo, amateka y’ubuvuzi, ibindi bizamini bya laboratoire, igisubizo cy’ubuvuzi, epidemiologiya nandi makuru .
    .Iyi reagent ikoreshwa gusa mugupima serumu na plasma. Ntishobora kubona ibisubizo nyabyo mugihe ikoreshejwe izindi ngero nka macandwe ninkari nibindi.

    IBIKORWA BIKORWA

    Umurongo 10-1000 gutandukana ugereranije: -15% kugeza kuri + 15%.
    Coefficient ihuza umurongo: (r) ≥0.9900
    Ukuri Igipimo cyo gukira kizaba kiri muri 85% - 115%.
    Gusubiramo CV≤15%

    RINGARUKA
    1.Shao, JL & F.Wu.Iterambere rya vuba muburyo bwo gutahura Helicobacter pylori [J] .Ikinyamakuru cya Gastroenterology na Hepatology, 2012,21 (8): 691-694

    2.Hansen JH, n'abandi. HAMA Kwivanga kwa Murine Monoclonal Antibody ishingiye kuri Immunoassays [J] .J ya Clin Immunoassay, 1993,16: 294-299.
    3.Levinson S.
    Urufunguzo rwibimenyetso byakoreshejwe:

     t11-1 Mubikoresho byubuvuzi bya Vitro
     tt-2 Uruganda
     tt-71 Ubike kuri 2-30 ℃
     tt-3 Itariki izarangiriraho
     tt-4 Ntugakoreshe
     tt-5 ICYITONDERWA
     tt-6 Baza Amabwiriza yo Gukoresha

    Xiamen Wiz Biotech CO., LTD
    Aderesi: Igorofa 3-4, Inyubako.16
    Tel: + 86-592-6808278
    Fax: + 86-592-6808279


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