Diagnostic Kit kuri 25-hydroxy vitamine d(Fluorescence Umungororomatografia Assay)
Kuberako muri vitro ikoresha gusa
Nyamuneka soma iyi paki witonze mbere yo gukoresha kandi ukurikize neza amabwiriza. Kwizerwa kw'ibisubizo Ibisubizo ntibishobora kwizerwa niba hari gutandukana mumabwiriza muriyi paki.

Gukoresha
Gusuzuma Ibikoresho 25-Hydroxy D (Fluorescence Imyunochromatograchic Assay) ni fluorescence imyume ya vitamine D.Niba isuzuma ryabafasha reagent.Icyitegererezo cyiza kigomba gushimangirwa nubundi buryo. Iki kizamini gigenewe gukoreshwa numwuga gusa.

Incamake
Vitamine D ni vitamine kandi nazo ni imisemburo ya steroyisi, ahanini ikubiyemo VD2 na VD3, imitsi irasa cyane. Vitamine D3 na D2 ihindurwa kuri Hydroxyl 25 ya HYTROBINL D (harimo 25-ditherxyl vitamine D3 na D2). 25- (OH) vd mumubiri wumuntu, kubaka ihamye, kwibanda cyane. 25- (OH) VD yerekana umubare wa vitamine D. Immunochromatography kandi irashobora gutanga ibisubizo muminota 15.

Ihame ry'uburyo
Ingano yikikoresho cyikizamini gifatanye na Conjugate ya BSA na 25- (OH) vd mugice cyikizamini na ihene yo kurwanya urukwavu igg antibod antibods akarere kagenzurwa. Marker Pad yashizwemo na fluorescence Mark Anti 25- (Oh) vd antibody ninkwavu igg mbere. Iyo ugerageza icyitegererezo, 25- (OH) vd in dopropro hamwe na fluorescence yerekanaga anti 25- (OH) vd antibody, kandi igace invance. Munsi y'ibikorwa bya Immunochromatography, urujya n'uruza rugana ku mpapuro zinjira, igihe bigoye kunyura mu gace ka 3- (OH) VM. Kwibanda kuri 25- Oh VD ni isano mbi kubimenyetso bya fluorescence, hamwe na 25- (OH) vd murugero rushobora kumenyekana na fluorescence Immonasas.

Reages nibikoresho byatanzwe

Ibikoresho 25t:
.Ikarita ikarita kugiti cye isenyutse hamwe na 25t
.Umuti wa 25t
.B Igisubizo 1
.Gukoreshamo ibice 1

Ibikoresho birakenewe ariko ntibitangwa
Icyitegererezo cyo gukusanya ibintu, igihe

Icyitegererezo cyo gukusanya no kubika
1.Icyitegererezo cyageragejwe gishobora kuba, Heparin anticogulant Plasma cyangwa EDTA Anticogulant Plasma.
2.Ubuhanga busanzwe bwo gukusanya icyitegererezo. Serum cyangwa Plasma Icyitegererezo gishobora gukomeza gukonjesha saa mbiri-8 ℃ ku minsi 7 na konko hepfo -15 ° C mumezi 6.
3.Icyitegererezo cyose irinde guhagarika inzinguzingo.

Uburyo bwo Gukwirakwiza
Uburyo bwikizamini mubikoresho reba igitabo cya Immwungowarzer. Uburyo bwikizamini cya reagent ni bukurikira
1.Ubundi ku ruhande reagents zose hamwe ningero kubushyuhe bwicyumba.
.
3.scan code yo gutesha agaciro kugirango yemeze ikintu cyibizamini.
4. Fata ikarita yikizamini kuva mu gikapu.
5.Mugukanda ikarita yikizamini mubibanza byamakarita, scan code ya QR, hanyuma umenye ikintu cyibizamini.
6.Add 30μl serumu cyangwa icyitegererezo cya plasma kubisubizo, hanyuma uvange neza.
7.Add 50μl B SCREROUTION KUBURYO BUKURIKIRA, hanyuma uvange neza.
8.Kureka imvange muminota 15.
9.ADD 80μl imvange kuri sample iriba ikarita.
.
11.Ibyiza ku nyigisho za Portwar Shonges yo gusesengura (Wiz-A101).

Indangagaciro ziteganijwe
25- (OH) vd urwego rusanzwe: 30-100ng / ml
Birasabwa ko buri laboratoire ishyiraho urwego rusanzwe rugereranya abarwayi bayo.

Ibisubizo n'ibisubizo
.Amakuru yavuzwe haruguru ni intera yerekanwe ku makuru yo kumenya ibi bikoresho, kandi birasabwa ko buri laboratoire ikwiye gushyiraho intera iboneye ku bijyanye n'ubushobozi bwa clinique y'abaturage bo muri kano karere.
.Ibitekerezo bya 25- (OH) hejuru birenze urugero, hamwe nimpinduka za physiologique cyangwa igisubizo cyo guhangayika kigomba gukurwaho.
.Ibisubizo byubu buryo bikoreshwa gusa kumirongo yerekanwe yashizweho nubu buryo, kandi ibisubizo ntabwo bigereranywa nuburyo butaziguye nubundi buryo.
.Ibintu birashobora kandi gutera amakosa kubisubizo, harimo impamvu za tekiniki, amakosa yimikorere nibindi bintu byintangarugero.

Kubika no gutuza
.Ibikoresho bifite amezi 18 yuzuye - ubuzima kuva gukora. Bika ibikoresho bidakoreshwa kuri 2-30 ° C. Ntugahagarike. Ntukoreshe kurenza itariki izarangiriraho.
.Ntabwo ukingure umufuka ufunze kugeza witeguye gukora ikizamini, kandi ikizamini cyo gukoresha kimwe gisabwa gukoreshwa (ubushyuhe 2-35 ℃, ubushyuhe bwa 2-35 birashoboka.
.Sample Diluennt ikoreshwa ako kanya nyuma yo gufungura.

Umuburo n'inyungu
.Ibikoresho bigomba gushyirwaho ikimenyetso no gukingirwa ubushuhe.
.Ibikoresho byiza bigenwa nubundi buryo.
.Ibigereranyo bizafatwa nkuwanduye.
.Ntukoreshe reagent yarangiye.
.Ntabwo uhanagura reagent mubikoresho hamwe na love hatandukanye oya ..
.Do ntukoreshe amakarita y'ibizamini hamwe nibikoresho byose bitashoboka.
.

LKwigana
.Kuvuga hamwe nigipimo icyo aricyo cyose gikoresha imbeba antibolies, ibishoboka bibaho kubavanga numuntu arwanya imbeba (Hama) murugero. Ingendo zituruka ku barwayi babonye imyiteguro ya antibodis ya monoclonal yo kwisuzumisha cyangwa kuvura bishobora kuba birimo hama. Ibyingenzi birashobora gutera ibisubizo byiza cyangwa ibinyoma.
.Ibisubizo byibizamini bigamije kuvugwa gusa, ntibigomba kuba ishingiro ryonyine ryo gusuzuma amavuriro no kuvurwa, Amateka y'ubuvuzi, mu yandi mateka, igisubizo cyo kuvura nandi makuru .
.Ibi bikoreshwa gusa kubizamini bya Serum nibizamini bya Plasma. Ntabwo ishobora kubona ibisubizo nyabyo iyo bikoreshwa mubindi byitegererezo nkamacandwe ninkari n'ikibi nibindi.

Ibiranga imikorere

RIbisobanuro

.
2.Levinson S. Imiterere ya antibodique ninshingano muri Impungo zivanze [j] .J wa Clin Imyungesay, 1992: 108-114.

Urufunguzo rwibimenyetso byakoreshejwe:

	Mugikoresho cyo kwivuza kwa vitro
	Uruganda
	Ububiko kuri 2-30 ℃
	Itariki yo kurangiriraho
	Ntukoreshe
	Kwitondera
	Ngera inama amabwiriza yo gukoresha

Xiamen wiz biotech co., Ltd
Aderesi: Igorofa 3-4, No.16 Amahugurwa yubuvuzi, Amahugurwa yubuvuzi, 2030 Wengjiao Umuhanda Wiburengerazuba, Akarere ka Haicang, 361026, Xiamen, Abapayiniya
Tel: + 86-592-6808278
Fax: + 86-592-6808279