IHame

Ibice by'igikoresho cyo kwipimisha bisizwe hamwe na conjugate ya BSA na 25- (OH) VD mukarere k'ibizamini hamwe na antibody y'ihene irwanya urukwavu IgG mukarere kayobora. Ikimenyetso cya marike gitwikiriwe na fluorescence ikimenyetso kirwanya antibody 25- (OH) VD hamwe ninkwavu IgG mbere. Iyo ugerageza icyitegererezo, 25- (OH) VD muri sample ikomatanya na fluorescence yanditseho antibody 25- (OH) VD, hanyuma igakora imvange yubudahangarwa. Mubikorwa byubudahangarwa bw'umubiri, urujya n'uruza rugana mu cyerekezo cy'impapuro zinjira, iyo complexe yatsinze akarere k'ibizamini, Ikimenyetso cya fluorescent yubusa kizahuzwa na 25- (OH) VD kuri membrane.Ubushuhe bwa 25- (OH) VD ni ihuriro ribi ryerekana ibimenyetso bya fluorescence, kandi ubunini bwa 25- (OH) VD murugero birashobora gutahurwa na fluorescence immunoassay assay.

Uburyo bw'ikizamini:

Nyamuneka soma igitabo gikoreshwa nigikoresho cyo gushyiramo mbere yo kugerageza.

1. Shyira ku ruhande reagent zose hamwe nicyitegererezo kubushyuhe bwicyumba.
2. Fungura Portable Immune Analyser (WIZ-A101), andika ijambo ryibanga rya konte ukurikije uburyo bwo gukora igikoresho, hanyuma winjire muburyo bwo kumenya.
3. Sikana kode ya dentification kugirango wemeze ikintu cyizamini.
4. Kuramo ikarita yikizamini mu mufuka.
5. Shyiramo ikarita yikizamini ahantu h'ikarita, suzuma kode ya QR, hanyuma umenye ikintu cyizamini.
6. Ongeramo 15μL serumu cyangwa plasma sample mubisubizo, hanyuma uvange neza
7. Ongeramo 80μL ivanze kugirango utange urugero rwikarita.
8. Kanda kuri bouton "isanzwe yikizamini", nyuma yiminota 15, igikoresho kizahita kimenya ikarita yikizamini, kirashobora gusoma ibisubizo bivuye mugice cyo kwerekana igikoresho, hanyuma wandike / wandike ibisubizo byikizamini.
9. Reba amabwiriza ya Portable Immune Analyser (WIZ-A101).