Igikoresho cyo gusuzuma（Inzahabu）kuri Calprotectin
Kuri vitro kwisuzumisha koresha gusa
Nyamuneka soma iyi paki shyiramo witonze mbere yo gukoresha kandi ukurikize neza amabwiriza. Ibisubizo byizewe ntibishobora kwemezwa niba hari gutandukana kwamabwiriza muriyi paki yinjizamo.

GUKORESHA
Igikoresho cyo kwisuzumisha kuri Calprotectin (cal) ni isuzuma rya zahabu ya immunochromatographic isuzuma kugirango hamenyekane inyana ziva mu mwanda w’abantu, zifite agaciro gakomeye ko gusuzuma indwara zifata amara. Iki kizamini ni reagent. Icyitegererezo cyiza cyose kigomba kwemezwa nubundi buryo. Iki kizamini kigenewe ubuvuzi bwumwuga gusa. Hagati aho, iki kizamini gikoreshwa kuri IVD, ibikoresho byinyongera ntibikenewe.

INCAMAKE
Cal ni heterodimer, igizwe na MRP 8 na MRP 14. Iba muri neutrophile cytoplasm kandi ikagaragarira kuri selile monon nuclear. Cal ni poroteyine ikaze, ifite icyiciro gihamye hafi icyumweru kimwe mumyanda yabantu, yiyemeje kuba ikimenyetso cyindwara yumura. Igikoresho ni ikizamini cyoroshye, cyerekanwa semiqualitative igaragaza inyana mumyanda yabantu, ifite sensibilité yo hejuru kandi yihariye. Ikizamini gishingiye ku buryo bwihariye bwihariye antibodies sandwich reaction ihame hamwe na zahabu immunochromatographic assay tekinike yo gusesengura, irashobora gutanga ibisubizo muminota 15.

IHame RY'UBURYO
Igice gifite anti cal coating McAb mukarere ka test hamwe na ihene irwanya urukwavu IgG antibody mukarere kayobora, ifatirwa kuri chromatografi ya membrane hakiri kare. Lable pad isizwe na zahabu ya colloidal yanditseho anti cal McAb na zahabu ya colloidal yanditseho urukwavu IgG antibody mbere. Iyo ugerageza icyitegererezo cyiza, inyana yicyitegererezo yahujwe na zahabu ya colloidal yanditseho anti cal McAb, kandi ikora urwego rwumubiri, kuko yemerewe kwimuka kuruhande rwibizamini, uruganda rwitwa cal conjugate rufatwa na anti cal coating McAb kuri membrane no kumiterere. “Ant cal coating McAb-cal-colloidal zahabu yanditseho anti cal McAb”, itsinda ryibizamini byamabara ryagaragaye mukarere ka test. Imbaraga zamabara zifitanye isano neza nibirimo inyana. Icyitegererezo kibi ntigitanga umurongo wikizamini kubera kubura zahabu ya colloidal conjugate cal complex. Ntakibazo cal ihari murugero cyangwa ntayo, hariho umurongo utukura ugaragara mukarere kerekanwe hamwe nakarere kagenzura ubuziranenge, bifatwa nkibipimo ngenderwaho byimbere mu gihugu.

REAGENTS N'IBIKORWA BYUZUWE
25T ibice：
.Ikarita yipimisha kugiti cyawe ifu yometse kuri desiccant
.Urugero rwimikorere: ibiyigize ni 20mM pH7.4PBS
.Dispette
. Shyiramo paki

IBIKORWA BISABWA ARIKO NTIBITANZWE

Icyitegererezo cyo gukusanya ibikoresho, igihe

GUKORANYA URUGERO N'UBubiko
Koresha ikintu gishobora gusukurwa kugirango ukusanye umwanda mushya, hanyuma ugerageze ako kanya. Niba bidashobora kugeragezwa ako kanya, nyamuneka ubitswe kuri 2-8 ° C kumasaha 12 cyangwa inzogera -15 ° C mumezi 4.

GUKORA UBURYO
1.Kuramo inkoni y'icyitegererezo, winjizwe mucyitegererezo cy'umwanda, hanyuma usubize inkoni y'icyitegererezo inyuma, uhindure neza kandi uzunguze neza, subiramo ibikorwa inshuro 3. Cyangwa ukoresheje icyitegererezo cyafashe urugero rwa 50mg rwicyitegererezo, hanyuma ugashyiramo umuyoboro wicyitegererezo urimo umwirondoro wicyitegererezo, hanyuma ugasunika neza.
2.Koresha icyitegererezo cya pipette ikoreshwa fata icyitegererezo cyoroshye cyumurwayi wimpiswi, hanyuma wongereho ibitonyanga 3 (hafi 100uL) kumuyoboro wa fecal sampling hanyuma uzunguze neza, ushire kuruhande.
3.Kuramo ikarita yikizamini mu mufuka wa file, uyishyire kumeza urwego hanyuma ushireho akamenyetso.
4.Kuraho ingofero muri sample ya sample hanyuma ujugunye ibitonyanga bibiri byambere byongeweho icyitegererezo, ongeramo ibitonyanga 3 (hafi 100uL) ntagituba cyinshi cyicyitegererezo cya verticaly hanyuma buhoro buhoro mucyitegererezo cyikarita hamwe na disiketi yatanzwe, tangira igihe.
5.Ibisubizo bigomba gusomwa muminota 10-15, kandi ntibyemewe nyuma yiminota 15.

IBISUBIZO BY'IKIZAMINI NO GUSOBANURA

Ububiko N'UBUHAMYA
Igikoresho ni amezi 24 yo kubaho-uhereye igihe cyakorewe. Bika ibikoresho bidakoreshwa kuri 2-30 ° C. Ntukingure umufuka ufunze kugeza igihe witeguye gukora ikizamini.
UMUBURO N'UBWITONDERWA
1.Ibikoresho bigomba gufungwa kandi bikarindwa ubushuhe¹.
2.Ntukoreshe icyitegererezo gishyizwe kure cyane cyangwa gukonjeshwa inshuro nyinshi no gukonjesha kugirango ugerageze
3.Urugero rwibanze rurakabije cyangwa ubunini burashobora gukora ibyitegererezo byikarita yikizamini, nyamuneka centrifuge icyitegererezo cyafashwe hanyuma ufate supernatant kugirango ugerageze.
4.Gukoresha nabi, urugero rwinshi cyangwa ruto rushobora kuganisha ku gutandukana.

LIMITATION
1.Ibisubizo by'ibizamini ni ibyavuzwe gusa, ntibigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura kwa muganga, imicungire y’amavuriro y’abarwayi igomba kwitabwaho byimazeyo hamwe n’ibimenyetso byayo, amateka y’ubuvuzi, ibindi bizamini bya laboratoire, ibisubizo by’ubuvuzi, epidemiologiya nibindi amakuru².
2.Iyi reagent ikoreshwa gusa mugupima fecal. Ntishobora kubona ibisubizo nyabyo mugihe ikoreshejwe izindi ngero nka macandwe ninkari nibindi.