Igikoresho cyo gusuzuma AmasokoCitonin

(Fluorescence Umungororomatografia Assay)

Kuberako muri vitro ikoresha gusa

Nyamuneka soma iyi paki witonze mbere yo gukoresha kandi ukurikize neza amabwiriza. Kwizerwa kw'ibisubizo Ibisubizo ntibishobora kwizerwa niba hari gutandukana mumabwiriza muriyi paki.

Gukoresha

Igikoresho cyo gusuzuma Amasoko (Fluorescence Imyunochromatograchic Assay) ni fluorescence imyumechromatografi Icyitegererezo cyose kigomba gushimangirwa nubundi buryo. Iki kizamini gigenewe gukoreshwa numwuga gusa.

Incamake

Amasoko agizwe na 116 aside amine hamwe nuburemere bwayo ni 12.7kd. PCT igaragarira na NeuroendoCrine kandi isenyuka na enzymes muri (immakura), carbox-guhagarika peptide, na amino bihagarika peptide. Abantu bafite ubuzima bwiza bafite gusa cyane muri maraso yabo, ishobora kwiyongera cyane nyuma yindwara za bagiteri. Iyo sepsis ibaye mumubiri, tissues nyinshi zishobora kwerekana pact, bityo pact irashobora gukoreshwa nkikimenyetso gikomeye cya sepsis. Ku barwayi bamwe banduye kwandura, PCT irashobora gukoreshwa nkigipimo cya antibiyotike no guca imanza.

Ihame ry'uburyo

Ingano y'ibikoresho by'ikizamini ikongejwe na antiped antibody mu karere k'ikizamini n'ihene anti rubbi urukwavu Igg antibod mu karere kagenzura. Amaduka yatowe na fluorescence yanditseho anti PCT antibody ninkwavu igg mbere. Iyo ugerageza icyitegererezo cyiza, Antigen ya PCT muri sample ihuza na fluorescence yanditseho anti pact antibody, hanyuma ukore imvange. Munsi y'ibikorwa bya Immunochromatography, urujya n'uruza rutemba mu mpapuro zinjira, igihe bigoye byanyuze mu karere k'ikizamini, yahujije hamwe na anti PCT igana antibody, ikora ikigo gishya. Urwego rwa PCT rufitanye isano neza nikimenyetso cya fluorescence, kandi kwibanda kuri PCT murugero rushobora kumenyekana na fluorescence Immonasas.