IHame

Ibice byibikoresho byipimishije bisizwe na antibody ya Cpn-IgM mukarere ka test hamwe na ihene irwanya urukwavu IgG antibody mukarere kayobora. Lable pad isizwe na fluorescence yanditseho antibody ya Cpn-IgM hamwe ninkwavu IgG mbere. Iyo ugerageza icyitegererezo cyiza, antigen ya Cpn-IgM muri sample ikomatanya na fluorescence yanditseho antibody ya Cpn-IgM, hanyuma igakora imvange yubudahangarwa. Mubikorwa bya immunochromatografiya, urujya n'uruza rwerekezo rwimpapuro zinjira. Iyo complexe yatsinze akarere k'ibizamini, yahujwe na anti-Cpn-IgM coating antibody, ikora urwego rushya. Umurongo utukura nicyo gisanzwe kigaragara ahantu hagenzurwa ubuziranenge (C) kugirango harebwe niba hari ingero zihagije kandi niba inzira ya chromatografiya ari ibisanzwe. Irakoreshwa kandi nkigenzura ryimbere kuri reagent.

Uburyo bwo Kwipimisha

Uburyo bwa test ya WIZ-A202 hamwe na Portable Immune isesengura WIZ-A101 bivuga amabwiriza y'amabwiriza. Uburyo bwo gukora ibizamini bugaragara nuburyo bukurikira:

1. Kuramo ikarita yikizamini mu mufuka wa file, uyishyire kumeza urwego hanyuma ushireho akamenyetso.
2. Ongeramo 10μl serumu cyangwa plasma sample cyangwa 20ul yuzuye yamaraso kugirango utange neza ikarita hamwe na disette yatanzwe, hanyuma ongeramo 100μl (hafi 2-3 igitonyanga) sample diluent; gutangira igihe.
3. Tegereza byibuze iminota 10-15 hanyuma usome ibisubizo, ibisubizo ntabwo byemewe nyuma yiminota 15.