IHame

Membrane yigikoresho cyipimishije yashizwemo antibody ya Tf mukarere kipimishije hamwe na ihene irwanya inkwavu IgG antibody mukarere kayobora. Lable pad isizwe na fluorescence yanditseho anti Tf antibody ninkwavu IgG mbere. Iyo ugerageza icyitegererezo cyiza, antigen ya Tf murugero ikomatanya na fluorescence yanditseho antibody ya Tf, hanyuma igakora imvange yumubiri. Mubikorwa bya immunochromatografiya, urujya n'uruza rwerekezo rwimpapuro zinjira. Iyo complexe yatsinze akarere k'ibizamini, yahujwe na anti-Tf coating antibody, ikora urwego rushya.

Niba ari bibi, icyitegererezo ntikirimo transferi cyangwa ibirimo biri hasi, kugirango ubudahangarwa bw'umubiri budashobora gushingwa. Nta murongo utukura uzaba ahantu hagaragara (T). Ntakibazo niba antibody ya Tf ibaho murugero cyangwa itabaho, ibisigaye bya colloidal zahabu yanditseho imbeba irwanya abantu IgM monoclonal antibody hamwe na antibody y'ihene irwanya imbeba IgG yometse kumurongo wo kugenzura ubuziranenge (C). Hanyuma agglutinates itezimbere ibara mugace kagenzura ubuziranenge, kandi umurongo utukura uzagaragara muri (C). Umurongo utukura nicyo gisanzwe kigaragara ahantu hagenzurwa ubuziranenge (C) kugirango harebwe niba hari ingero zihagije kandi niba inzira ya chromatografiya ari ibisanzwe. Irakoreshwa kandi nkigenzura ryimbere kuri reagent.

Uburyo bwo Kwipimisha

Nyamuneka soma paki shyiramo mbere yo kugerageza.

1.Kuramo ikarita yikizamini mu gikapu cya file, uyishyire kumeza urwego hanyuma ushireho akamenyetso.

2. Kuraho ingofero muri sample ya sample hanyuma ujugunye ibitonyanga bibiri byambere byongeweho icyitegererezo, ongeramo ibitonyanga 3 (hafi 100uL) ntagituba cyinshi cyerekanwe cyerekezo gihagaritse kandi gahoro gahoro murugero rwikarita yatanzwe. Noneho tangira igihe.