Ihame

Ikizamini cyibizamini gikongejwe hamwe na Rotavirus itsinda rya Antigen mukarere kakiza kandi ihene anti rabbit igg antibod mukarere. Amaduka yatowe na fluorescence yanditseho anti rotavirus itsinda a nigg igg mbere. Mugihe ugerageza icyitegererezo cyiza, rv in dople ihuza na fluorescence yanditseho indogobe ya anti rotavirus a, kandi igace invance. Munsi y'ibikorwa bya Immunochromatography, urujya n'uruza rutemba mu cyerekezo cy'impapuro zinjira. Igihe kigorekanega akarere kakizamini, ihujwe n'amatsinda arwanya Rotavirus antibody, ikora ikigo gishya. Niba ari bibi, nta tsinda rya Rotavirus rihari kuri antigen murugero, kugirango ubudahangarwa budashobora gushingwa, ntihazabaho umurongo utukura muburyo bwo gutahura (t). Utitaye ko hari itsinda rya Rotavirus rihari muri spertimen, imbeba yanditseho ig ni chromatoje ahantu heza (c) kandi ifata nimbeba anti-imbeba igg antibody. Umurongo utukura uzagaragara mukarere keza (c). Umurongo utukura ni star star igaragara ahantu hagenzurwa neza (C) kugirango ikemure niba hari ingero zihagije kandi niba inzira ya chromatografiya ari ibisanzwe. Irakoreshwa kandi nkibipimo ngenderwaho byimbere kuba reage.

Uburyo bw'ikizamini:

1.Ibibazo byabarwayi bagomba gukusanywa. Nk'uko amakuru abitangaza, kwishyiriraho cyane Rotavirus mu kwamamaza abarwayi bakoresheje gastroenteritis biboneka nyuma yiminsi 3-5 nyuma yiminsi 3-13 nyuma yiminsi 3-13 nyuma yikibazo. Niba icyitegererezo cyegeranijwe igihe kinini impiswi, umubare wa antigens ntushobora kuba ahagije kugirango ube ibintu byiza.

2.Icyitegererezo kigomba gukusanywa muburyo busukuye, bwumutse, bwumutse butarimo amazi kidahari kidahari kandi kibanziriza.

3.Kuri abarwayi batari impiswi, umwambaro wakusanyije ntugomba kuba munsi ya garama 1-2. Ku barwayi barwaye impiswi, niba umwanda umazi, nyamuneka ukusane byibuze ml 1-2 yumukunzi. Niba umwanda urimo amaraso menshi na mucus, nyamuneka ongera ukusangire icyitegererezo.

4.Bisabwa kugerageza ingero zihita nyuma yo gukusanya, bitabaye ibyo bigomba koherezwa muri laboratoire mumasaha 6 kandi babitswe muri 2-8 ° C. Niba ibyitegererezo bitageragejwe mumasaha 72, bagomba kubikwa ku bushyuhe bukurikira -15 ° C.

5. Koresha umwanda mushya wo kwipimisha, nuwambure urugero rwivanze na Dilunt cyangwa amazi yatoboye