IHame

Ibice by'igikoresho cyo kwipimisha bisizwe na microalbumin antigen mu karere k'ibizamini hamwe n'ihene irwanya urukwavu IgG antibody ku karere kayobora. Lable pad isizwe na fluorescence yanditseho microalbumin ninkwavu IgG mbere. Niba nta alubumu iri mu nkari, zahabu ya colloidal yanditseho anti-Alb-yanditseho antibody ya monoclonal antibody ku mpapuro za zahabu ya colloidal izajya ikorera kuri membrane hamwe n'inkari kugera kumurongo wo gutahura, hanyuma igahuza na antigen yometse kuri Albine hamwe na antigen igaragara. umurongo. Kandi ibara ryumurongo ryijimye kuruta ibara ryumurongo mugace kayobora (C), ibi nibisubizo bibi. Niba inkari zirimo alubumu, zizahangana na antigen yometse kuri antigen kuri membrane kugirango ihuze imbuga za antibody nkeya kuri colloidal zahabu yanditseho anti-Alb-yanditseho antibody ya monoclonal. Nkuko ingano ya albumine mu nkari yiyongera, kwipimisha

Ibara ryumurongo rizahinduka ryoroshye. Ibiri muri alubumu mu nkari birashobora kumenyekana igice cya kabiri ugereranije agace kerekana (T) hamwe nubugenzuzi (C). Agace kagenzura ubuziranenge (C) hamwe n’ahantu (R) ku gikoresho bizahora bigaragara mugihe cyizamini, kandi ntaho bihuriye no kuba hariho inkari za alubumu. Agace kagenzura (C) hamwe nu gace kerekana (R) umurongo urashobora gukoreshwa nkimbere yimbere yo kugenzura ibipimo ngenderwaho kubikoresho.

Uburyo bw'ikizamini:

Nyamuneka soma igitabo gikoreshwa nigikoresho cyo gushyiramo mbere yo kugerageza. Gukuramo icyitegererezo kubushyuhe bwicyumba mbere yo gukoresha.

1.Kuramo ikarita yikizamini mu gikapu. Shyira hejuru hejuru ya horizontal hanyuma ushireho akamenyetso.

2.Fata icyitegererezo cy'inkari hamwe na pipette ikoreshwa, ujugunye ibitonyanga bibiri byambere by'icyitegererezo cy'inkari. Ongeraho ibitonyanga 3 (hafi 100uL) byinkari zidafite ububobere hagati yumwobo wikitegererezo wikarita yikizamini uhagaritse hanyuma utangire igihe.

3.Soma ibisubizo muminota 10-15. Bitemewe niba iminota irenze 15.