Twishimiye umwanya mwiza cyane mubyifuzo byacu kubicuruzwa byacu byiza byujuje ubuziranenge, igiciro cyo gupiganwa hamwe na serivise nziza kumyaka 8 yohereza ibicuruzwa mubushinwa nezaIkizamini cyo Gusuzuma ByihuseIbizamini Byihuse mubikoresho byisesengura rya Pathologiya, Twizeye ubwacu ko hazafatwa ibyiringiro bizaza kandi turizera ko dushobora kugirana ubufatanye burambye nibitekerezo biturutse hirya no hino mubidukikije.
Twishimiye umwanya mwiza cyane mubyifuzo byacu kubicuruzwa byacu byiza byujuje ubuziranenge, igiciro cyo gupiganwa hamwe na serivisi nziza kuriUbushinwa Byihuse, Ikizamini cyo Gusuzuma Byihuse, Ibicuruzwa byacu birazwi cyane mwijambo, nka Amerika yepfo, Afrika, Aziya nibindi. Amasosiyete "gukora ibicuruzwa byo mucyiciro cya mbere" nkintego, kandi yihatire guha abakiriya ibisubizo byujuje ubuziranenge, gutanga serivisi nziza nyuma yo kugurisha no kugoboka tekinike, hamwe ninyungu zabakiriya, bihanga umwuga mwiza nigihe kizaza!
Kuri vitro kwisuzumisha koresha gusa

Nyamuneka soma iyi paki shyiramo witonze mbere yo gukoresha kandi ukurikize neza amabwiriza. Ibisubizo byizewe ntibishobora kwemezwa niba hari gutandukana kwamabwiriza muriyi paki yinjizamo.

GUKORESHA

Igikoresho cyo gusuzuma indwara ya Hepatitis C Virus Antibody (Fluorescence Immunochromatographic Assay) ni isuzuma rya fluorescence immunochromatographic igamije kumenya umubare wa antibody ya HCV muri serumu yumuntu cyangwa plasma, ibyo bikaba ari ingirakamaro zifasha kwisuzumisha kwandura indwara ya hepatite C.Icyitegererezo cyiza cyose kigomba kwemezwa nabandi uburyo. Iki kizamini kigenewe ubuvuzi bwumwuga gusa

1. Shira ku ruhande reagent zose hamwe nicyitegererezo kubushyuhe bwicyumba.
2.Fungura Portable Immune Analyser (WIZ-A101), andika ijambo ryibanga rya konte ukurikije uburyo bwo gukora igikoresho, hanyuma winjire muburyo bwo gutahura.
3.Shobora kode ya dentification kugirango wemeze ikintu cyizamini.
4.Kuramo ikarita yikizamini mu mufuka wa file.
5. Shyiramo ikarita yikizamini ahantu h'ikarita, suzuma kode ya QR, hanyuma umenye ikintu cyizamini.
6. Ongeramo 20μL serumu cyangwa plasma icyitegererezo kugirango utange urugero, hanyuma uvange neza ..
7. Ongeraho 80μL igisubizo cyicyitegererezo kugirango utange neza ikarita.
8.Kanda buto ya "ikizamini gisanzwe", nyuma yiminota 15, igikoresho kizahita kimenya ikarita yikizamini, irashobora gusoma ibisubizo bivuye kuri ecran yerekana igikoresho, hanyuma wandike / wandike ibisubizo byikizamini.
9. Reba ku mabwiriza ya Portable Immune Analyser (WIZ-A101).

INCAMAKE

Virusi ya Hepatitis C (HCV) ni ibahasha, virusi imwe ya RNA (9.5 kb) ikomoka mu muryango wa Flaviviridae. Hatanzwe genotypes esheshatu nuruhererekane rwubwoko bwa HCV. Akato mu 1989, HCV ubu izwi nk'impamvu nyamukuru itera guterwa bifitanye isano na hepatite itari A, itari B. Indwara irangwa nuburyo bukaze kandi budakira. Abantu barenga 50% banduye barwara cyane, byangiza ubuzima bwa hepatite idakira hamwe na cirrhose yumwijima na kanseri ya hepatocellular. Kuva hashyirwaho mu 1990 hagamijwe kurwanya anti-HCV yo gutanga amaraso, umubare w'iyi ndwara ku bahabwa amaraso wagabanutse ku buryo bugaragara. Ubushakashatsi bw’ubuvuzi bwerekana ko umubare munini w’abantu banduye HCV bakora antibodiyite kuri poroteyine ya NS5 idafite imiterere. Kubwibyo, ibizamini birimo antigene zo mu karere ka NS5 ka genome ya virusi hiyongereyeho NS3 (c200), NS4 (c200) na Core (c22).

IHame RY'UBURYO

Ikibumbano cyibikoresho byo kwipimisha cyashizwemo antigen ya HCV mukarere kipimishije hamwe na antibody yihene irwanya urukwavu IgG mukarere kayobora. Lable pad isizwe na fluorescence yanditseho HCV antigen hamwe ninkwavu IgG mbere. Iyo ugerageza icyitegererezo cyiza, antibody ya HCV murugero ihuza na fluorescence yanditseho antigen ya HCV, hanyuma igakora imvange yumubiri. Mubikorwa bya immunochromatografiya, urujya n'uruza rugana mu cyerekezo cyimpapuro zinjira, iyo complexe yatsinze akarere k’ibizamini, ihuza na antigen ya HCV antigen coating antigen, ikora urwego rushya. Urwego rwa antibody ya HCV rufitanye isano neza n’ikimenyetso cya fluorescence, hamwe no kwibanda kwa Antibody ya HCV murugero irashobora gutahurwa na fluorescence immunoassay assay

REAGENTS N'IBIKORWA BYUZUWE

25T ibice：
.Ikarita yipimisha kugiti cyawe ifu yometse kuri desiccant
.Urugero rwimvugo
. Shyiramo paki

IBIKORWA BISABWA ARIKO NTIBITANZWE
Icyitegererezo cyo gukusanya ibikoresho, igihe
GUKORANYA URUGERO N'UBubiko
1.Ingero zapimwe zishobora kuba serumu, heparin anticoagulant plasma cyangwa plasma ya EDTA anticoagulant.
2.Kurikije tekiniki zisanzwe zegeranya icyitegererezo. Icyitegererezo cya serumu cyangwa plasma birashobora kubikwa muri firigo kuri 2-8 ℃ muminsi 7 hamwe no kubika munsi -15 ° C mumezi 6
3.Icyitegererezo cyose wirinde gukonjesha.

GUKORA UBURYO
Nyamuneka soma igitabo gikoreshwa nigikoresho cyo gushyiramo mbere yo kugerageza.
.Ibisubizo by'ibizamini ni ibyerekeye ivuriro gusa, ntibigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura kwa muganga, imicungire y’amavuriro y’abarwayi igomba kwitabwaho byimazeyo hamwe n’ibimenyetso byayo, amateka y’ubuvuzi, ibindi bizamini bya laboratoire, igisubizo cy’ubuvuzi, epidemiologiya nandi makuru .
.Iyi reagent ikoreshwa gusa mugupima serumu na plasma. Ntishobora kubona ibisubizo nyabyo mugihe ikoreshejwe izindi ngero nka macandwe ninkari nibindi.

IBIKORWA BIKORWA

INGINGO
1.Hepatite yo guterwa. Muri: Moore SB, ed. Indwara ziterwa na virusi. Alington, VA. Am. Assoc. Amabanki yamaraso, pp. 53-38.
2.Hansen JH, n'abandi. HAMA Kwivanga kwa Murine Monoclonal Antibody ishingiye kuri Immunoassays [J] .J ya Clin Immunoassay, 1993,16: 294-299.
3.Levinson S.
4.Hindura HJ., Purcell RH, Ubuholandi PV, nibindi. (1978) Indwara yandura muri hepatite itari A, itari B. Lancet I: 459-463.
5.Choo QL, Weiner AJ, Overby LR, Kuo G, Houghton M. (1990) Virusi ya Hepatitis C: intandaro nyamukuru itera virusi itari A, hepatite itari B. Br Med Bull 46: 423-441.
6.Engvall E, Perlmann P. (1971) Enzyme ihuza immunosorbent assay (ELISA): igipimo cyiza cya IgG. Immunochemie 8: 871-874.

AGACIRO KATEGANYIJWE

HCV-Ab <0.02

Birasabwa ko buri laboratoire ishyiraho urwego rwayo rusanzwe ruhagarariye abaturage barwaye.

IBISUBIZO BY'IKIZAMINI NO GUSOBANURA

• Aya makuru yavuzwe haruguru ni ibisubizo by’ikizamini cya reagent ya HCV-Ab, kandi hasabwa ko buri laboratoire igomba gushyiraho indangagaciro zitandukanye zo gutahura HCV-Ab zibereye abaturage bo muri aka karere. Ibisubizo byavuzwe haruguru nibisobanuro gusa.
• Ibisubizo byubu buryo birakoreshwa gusa murwego rwerekanwe muri ubu buryo, kandi ntaho bihuriye nubundi buryo.
• Ibindi bintu birashobora kandi gutera amakosa mubisubizo byo gutahura, harimo impamvu za tekiniki, amakosa yimikorere nibindi bintu byintangarugero.

Ububiko N'UBUHAMYA

1. Igikoresho ni amezi 18 yo kubaho-uhereye igihe byakorewe. Bika ibikoresho bidakoreshwa kuri 2-30 ° C. NTUBUNTU. Ntugakoreshe kurenza itariki izarangiriraho.
2. Ntukingure umufuka ufunze kugeza igihe witeguye gukora ikizamini, kandi ikizamini kimwe cyo gukoreshwa gisabwa gukoreshwa munsi y’ibisabwa (ubushyuhe 2-35 ℃, ubuhehere 40-90%) mu minota 60 byihuse bishoboka. .
3. Urugero rwa diluent rukoreshwa ako kanya nyuma yo gufungura.

UMUBURO N'UBWITONDERWA
.Ibikoresho bigomba gufungwa kandi bikarindwa ubushuhe.
.Ingero zose nziza zemezwa nubundi buryo.
.Ingero zose zifatwa nkibishobora guhumanya.
.NTUKORE reagent yarangiye.
.NTIMUGANIRE reagent mubikoresho bifite ubufindo butandukanye Oya ..
.NTUGASUBIZE gukoresha amakarita yikizamini hamwe nibindi bikoresho byose.
.Gukoresha nabi, urugero rwinshi cyangwa ruto rushobora kuganisha ku gutandukana.

LKwigana
.Kimwe nubushakashatsi ubwo aribwo bwose bukoresha antibodi yimbeba, birashoboka kubaho kubangamira antibodiyite zabantu barwanya imbeba (HAMA) murugero. Ingero z'abarwayi bahawe imyiteguro ya antibodiyite ya monoclonal yo gusuzuma cyangwa kuvura irashobora kuba irimo HAMA. Ingero nkizo zishobora gutera ibinyoma byiza cyangwa ibinyoma bibi.
Urufunguzo rwibimenyetso byakoreshejwe:

	Mubikoresho byubuvuzi bya Vitro
	Uruganda
	Ubike kuri 2-30 ℃
	Itariki izarangiriraho
	Ntugakoreshe
	ICYITONDERWA
	Baza Amabwiriza yo Gukoresha