MFUNDO

Mzerewu uli ndi anti cal coating McAb pagawo loyesa komanso anti-Rabbit IgG antibody pachigawo chowongolera, chomwe chimamangiriridwa ku membrane chromatography pasadakhale. Lable pad amakutidwa ndi golide wa colloidal wolembedwa kuti anti cal McAb ndi golide wa colloidal wotchedwa IgG antibody ya kalulu. Mukayesa zitsanzo zabwino, ma cal mu zitsanzo adapangidwa ndi golide wa colloidal olembedwa kuti anti cal McAb, ndikupanga chitetezo chamthupi, chifukwa amaloledwa kusuntha motsatira mzere woyeserera, cal conjugate complex imagwidwa ndi anti cal coating McAb pa nembanemba ndi mawonekedwe. "anti cal coating McAb-cal-colloidal gold lolembedwa kuti anti cal McAb", gulu loyesera lamitundumitundu lidawonekera pagawo loyesa. Kuchuluka kwamtundu kumalumikizidwa bwino ndi zomwe zili ndi cal. Chitsanzo cholakwika sichimapanga gulu loyesera chifukwa chosowa colloidal gold conjugate cal complex. Ziribe kanthu kuti cal ilipo mu zitsanzo kapena ayi, pali mizere yofiyira yomwe imawonekera pagawo lolozera komanso gawo lowongolera, lomwe limatengedwa ngati miyezo yamabizinesi amkati.

Njira Yoyesera:

1. Tulutsani ndodo, ikani mu ndowe, kenaka bwezerani ndodoyo, pukuta mwamphamvu ndikugwedezani bwino, bwerezani zomwezo katatu. Kapena kugwiritsa ntchito ndodo yongotengera ndodo ya 50mg ya ndowe, ndikuyika mu chubu la ndowe lomwe lili ndi dilution, ndikupukuta mwamphamvu.

2. Gwiritsani ntchito zitsanzo za pipette zotayidwa tengani ndowe zoonda kwambiri za wodwala matenda otsekula m'mimba, kenaka onjezerani madontho atatu (pafupifupi 100uL) ku chubu cha ndowe ndikugwedezani bwino, ikani pambali.

3.Tulutsani khadi loyesera kuchokera m'thumba la zojambulazo, liyikeni pa tebulo lapamwamba ndikulemba chizindikiro.

4.Chotsani kapu kuchokera ku chubu lachitsanzo ndikutaya madontho awiri oyambirira osungunuka, onjezerani 4.drops (pafupifupi 100uL) palibe bubble diluted sample verticaly ndi pang'onopang'ono mu chitsanzo chabwino cha khadi ndi dispette yoperekedwa, yambani nthawi.

5.Zotsatira ziyenera kuwerengedwa mkati mwa mphindi 10-15, ndipo ndizosavomerezeka pakatha mphindi 15.