MFUNDO

Mzerewu uli ndi antigen yotchinga ya MP-Ag pamalo oyesera ndi anti-mouse IgG antibody pagawo lowongolera, yomwe imamangiriridwa ku membrane chromatography pasadakhale. Lable pad imakutidwa ndi golide wa colloidal wolembedwa mbewa-anti human IgM McAb pasadakhale. Mukayesa zitsanzo zabwino, MP-IgM mu zitsanzo imaphatikizana ndi golide wa colloidal wotchedwa IgM McAb wa mbewa, ndikupanga chitetezo chamthupi. Pansi pa zochita za immunochromatography, zovuta ndi zitsanzo mkati mwa nembanemba ya nitrocellulose zimayenda molunjika ku pepala loyamwa, pamene zovutazo zidadutsa dera loyesa, zimaphatikizana ndi antigen yopaka MP-Ag, ndikupanga "MP-Ag yokutira antigen-MP. -IgM-colloidal golide yolembedwa kuti mbewa-anti human IgM McAb”, gulu loyesera lamitundumitundu lidawonekera pagawo loyesa. Chitsanzo choyipa sichimapanga gulu loyesera chifukwa chosowa chitetezo chamthupi. Ziribe kanthu kuti MP-IgM ilipo mu zitsanzo kapena ayi, pali mizere yofiyira yomwe imawonekera pagawo lowongolera, lomwe limawonedwa ngati miyezo yamabizinesi amkati.

Njira Yoyesera:

Njira yoyesera ya WIZ-A101 iwona malangizo a chitetezo chamthupi chosanthula. Njira yoyeserera yowonera ili motere:

1. Ikani pambali ma reagents ndi zitsanzo ku kutentha kwa chipinda.
2. Tulutsani khadi loyesera mu thumba la zojambulazo, liyikeni pa tebulo lapamwamba ndikuyika chizindikiro.
3. Onjezani 10μL seramu kapena madzi a m'magazi kapena 20μL yamagazi athunthu kuti muyese bwino pakhadi lomwe lili ndi dispette, kenaka yikani 100μL (pafupifupi 2-3 dontho) chitsanzo chochepetsera, yambani nthawi.
4. Dikirani kwa mphindi zosachepera 10-15 ndikuwerenga zotsatira zake, zotsatira zake ndizosavomerezeka pakatha mphindi 15.