MFUNDO

Nembanemba ya chipangizo choyesera imakutidwa ndi ma antibody a Hp-Ab pamalo oyeserera ndi anti-Rabbit IgG antibody pagawo lowongolera. Lable pad amakutidwa ndi fluorescence olembedwa anti Hp-Ag ndi kalulu IgG pasadakhale. Mukayesa zitsanzo zabwino, Hp-Ab mu zitsanzo imaphatikizana ndi fluorescence yotchedwa anti Hp-Ag, ndikupanga kusakaniza kwa chitetezo chamthupi. Pansi pa zochita za immunochromatography, zovuta kuyenda mu malangizo a absorbent pepala. Zovuta zikadutsa gawo loyesa, zimaphatikizidwa ndi anti-Hp-Ag zokutira zoteteza, zimapanga zovuta zatsopano. Ngati zili zoipa, palibe HP antibody mu chitsanzo, kotero kuti zovuta za chitetezo cha mthupi zisapangidwe, sipadzakhala mzere wofiira pamalo ozindikira (T). Mzere wofiyira ndi muyezo womwe umawonekera m'dera lowongolera (C) kuti muwone ngati pali zitsanzo zokwanira komanso ngati njira ya chromatography ndi yabwinobwino. Amagwiritsidwanso ntchito ngati muyezo wowongolera mkati mwa ma reagents.

Njira Yoyesera:

Chonde werengani phukusili musanayese.

1. Tulutsani khadi loyesera mu thumba la zojambulazo, liyikeni pa tebulo lapamwamba ndikuyika chizindikiro.

2. Onjezani madontho awiri a seramu kapena madzi a m'madzi a m'magazi (kapena madontho atatu a magazi athunthu/nsonga yamagazi) kuti muyese bwino pakhadi lomwe lili ndi dispette, kenaka yikani dontho limodzi lachitsanzo chosungunula, yambani nthawi.

3. Dikirani kwa mphindi zosachepera 10-15 ndikuwerenga zotsatira mu mphindi 10-15. Zotsatira zake sizabwino pakadutsa mphindi 15.