Chidule

Vitamini D ndi vitamini ndipo alinso mahomoni a steroid, makamaka vd2 ndi vd3, yemwe akuphatikiza kwake ndi zofanana kwambiri. Vitamini D3 ndi D2 amasinthidwa kukhala 25 hydroxyl vitamini d (kuphatikiza 25-dihhdroxyl vitamini d3 ndi d2). 25- (oh) VD m'thupi la munthu, magawande, chidwi chachikulu. 25- immunochromatograph ndipo imatha kupereka zotsatira zosakwana mphindi 15.

Mfundo ya njirayi

Membrane wa chipangizo choyeserera ndi cholumikizidwa ndi conjugate cha BSA ndi 25- (oh) vd kudera loyesa ndi Got antibit Antib IGG Dera Loyang'anira. Marker Pad ndi okutidwa ndi fluoresiscence chizindikiritso 1- (oh) vd antibody ndi kalulu igg pasadakhale. Mukamayesa zitsanzo, 25- (o) VD muyezo wosakira ndi fluresiscence adalemba anti 25- (oh) vd antibody, ndikupanga osakaniza. Pochitapo kanthu kwa immunoromatography, kulowerera kolowera m'mphepete mwa njira yoyesera, malo oyeserera a fluorescent, (oh) vd pa nembanemba. VD ndi kuphatikiza kolakwika kwa alurescence chizindikiro cha 25- (oh) vd mu zitsanzo zitha kupezeka ndi flureorescence immunoscesm Immunoassisy.