Maganizo

Membrane wa chipangizo choyeserera amaphatikizidwa ndi anti EV71 antibody kudera loyesa ndi Got antibit Antib IGG Dera Loyang'anira. Pad pad ndi wokutidwa ndi fluorescence yolembedwa anti Ev71 antibidy ndi kalulu igg pasadakhale. Mukamayesa zitsanzo zabwino, antigen antigen amasamba mwachitsanzo ndi fluresiscence yotchedwa Anti Ev71 antibody, ndikupanga osakaniza. Pakuchita za chromatography, zovuta kuyendayenda polowera mapepala, pomwe dera loyezetsa, linaphatikizana ndi anti EV71 akuphika antibody, amapanga mitundu yatsopano.

Ngati ndi zoipa, zitsanzozi sizikhala ndi leniborrus 71 zing'onozing'ono, kotero kuti zovuta kapena zodetsa sizingapangidwe. Sipadzakhala mzere wofiira pamalo opezeka (T). Ziribe kanthu kaya muli ngati awetovirus 71 antibody alipo mu fanizo kapena ayi, nyumba yotsala ya anti-Harloidal-anti-anti-mbewa igg antibodle yovomerezeka (C) kumanga. Kenako ogglutis amakula utoto mu malo owongolera, ndipo mzere wofiira uwonekera mu (c). Mzere wofiyira ndi wofanana umapezeka m'malo owongolera (c) poweruza ngati pali zitsanzo zokwanira komanso ngati njira yachilimweyo ndi yachilendo. Amagwiritsidwanso ntchito ngati njira yolamulira yamkati.

Njira Yoyeserera:

1.Mzitsanzo zimayesedwa kungakhale magazi athunthu, kuphatikizapo magazi osoweka kapena magazi otumphulika. Magazi athunthu sangasungidwe atasonkhanitsa. Ndiyenera kugwiritsidwa ntchito posangalalira.

2. Phwando la SATRUM limasonkhanitsidwa molingana ndi maluso olondola. Seramu yothira kutentha silingagwiritsidwe ntchito. Sitikulimbikitsidwa kugwiritsa ntchito lipemi, Turbid kapena Wodetsedwa seramu. Tinthu tating'onoting'ono ku seramu. Ndipo mpweya wabwino umakhudza zotsatira za mayeso, zitsanzo zoterezi ziyenera kukhala zamitundu kapena yosasankhidwa musanagwiritse ntchito.

3.Maso oyesedwa amatha kukhala heparin, sodium citrate kapena edta anticoagulant plasma.

4.Kugwiritsa ntchito njira zowerengera kuti atole zitsanzo. Seramu kapena plasma zitsanzo zimatha kusungunuka mu 2-8 ℃ kwa masiku atatu ndi khwalala m'munsimu - 15 c kwa miyezi itatu.

5.All Sample Pewani kuzungulira kwa miyala yamkuntho.