MFUNDO

Nembanemba ya chipangizo choyesera imakutidwa ndi anti EV71 antibody pagawo loyesa ndi anti-rabbit IgG antibody pagawo lowongolera. Ma lable pad amakutidwa ndi fluorescence olembedwa anti EV71 antibody ndi kalulu IgG pasadakhale. Mukayesa zitsanzo zabwino, antigen ya EV71 mu zitsanzo imaphatikizana ndi fluorescence yotchedwa anti EV71 antibody, ndikupanga kusakaniza kwa chitetezo chamthupi. Pansi pa zochita za chromatography, kuyenda movutikira molunjika ku pepala loyamwa, pomwe zovuta zidadutsa gawo loyesa, kuphatikiza ndi anti EV71 zokutira antibody, zimapanga zovuta zatsopano.

Ngati zili zoipa, chitsanzocho sichikhala ndi antibody ya enterovirus 71 IgM, kotero kuti chitetezo cha mthupi sichingapangidwe. Sipadzakhala mzere wofiira pamalo ozindikira (T). Ziribe kanthu kuti antibody ya Enterovirus 71 IgM ilipo pachitsanzochi kapena ayi, mbewa yotsalira ya IgM yokhala ndi golide yotsalira ya anti-munthu IgM ndi anti-mbewa IgG antibody yomwe idakutidwa pamalo owongolera (C) amamanga. Kenako ma agglutinates amapanga mtundu m'dera lowongolera, ndipo mzere wofiira udzawonekera mu (C). Mzere wofiyira ndi muyezo womwe umawonekera m'dera lowongolera (C) kuti muwone ngati pali zitsanzo zokwanira komanso ngati njira ya chromatography ndi yabwinobwino. Amagwiritsidwanso ntchito ngati muyezo wowongolera mkati mwa ma reagents.

Njira Yoyesera:

1.Zitsanzo zoyesedwa zimatha kukhala magazi athunthu, kuphatikiza magazi a venous kapena magazi a Peripheral. Magazi athunthu sangathe kusungidwa atatolera. Ndiyenera kugwiritsidwa ntchito ndikangotolera.

Zitsanzo za 2.Serum zimasonkhanitsidwa mwachisawawa malinga ndi njira zamakono. Seramu yotsekedwa ndi kutentha singagwiritsidwe ntchito. Sitikulimbikitsidwa kugwiritsa ntchito lipemic, turbid kapena seramu yowonongeka. Zinthu zomwe zili mu seramu. Ndipo mvula idzakhudza zotsatira zoyesa, zitsanzo zotere ziyenera kuyikidwa pakatikati kapena kusefedwa musanagwiritse ntchito.

3.Zitsanzo zoyesedwa zingakhale heparin, Sodium citrate kapena EDTA anticoagulant plasma.

4.Malinga ndi njira zoyenera sonkhanitsani zitsanzo. Seramu kapena plasma chitsanzo akhoza kusungidwa mufiriji pa 2-8 ℃ kwa masiku 3 ndi cryopreservation pansi -15 ° C kwa miyezi 3.

5.Zitsanzo zonse zimapewa kuzizira kozizira.