MFUNDO

Nembanemba ya chipangizo choyesera imakutidwa ndi antigen ya microalbumin pagawo loyesa ndi anti-Rabbit IgG antibody pagawo lowongolera. Ma lable pad amakutidwa ndi fluorescence otchedwa microalbumin ndi kalulu IgG pasadakhale. Ngati mumkodzo mulibe albumin, anti-Alb-labeled monoclonal antibody yolembedwa ndi golide papepala la golide wa colloidal idzayenda pa nembanemba ndi mkodzo kupita kumalo ozindikira, ndikuphatikizana ndi Alb-coated antigen yokhala ndi chowoneka. mzere. Ndipo mtundu wa mzere ndi wakuda kuposa mtundu wa mzere mu malo olamulira (C), izi ndi zotsatira zoipa. Ngati mkodzo uli ndi albumin, amapikisana ndi antigen yotsekedwa ndi Alb pa nembanemba kuti amangirire ku malo ochepa a antibody omwe ali pa colloidal gold-labeled anti-Alb-labeled monoclonal antibody. Pamene kuchuluka kwa albumin mu mkodzo kumawonjezeka, kuyesa

Mtundu wa mzere udzakhala wopepuka komanso wopepuka. Zomwe zili mu albumin mumkodzo zimatha kuzindikirika pang'onopang'ono poyerekeza malo ozindikira (T) ndi malo owongolera (C). Malo olamulira khalidwe (C) ndi malo ofotokozera (R) pa zida zidzawonekera nthawi zonse poyesa, ndipo alibe chochita ndi kukhalapo kwa albumin ya mkodzo. Malo olamulira (C) ndi malo owonetsera (R) angagwiritsidwe ntchito ngati ndondomeko yowonetsera khalidwe lamkati la zida.

Njira Yoyesera:

Chonde werengani buku lothandizira zida ndi kuyika phukusi musanayese. Thaw zitsanzo kutentha kwa chipinda musanagwiritse ntchito.

1.Tulutsani khadi loyesera muthumba la zojambulazo. Ikani mosabisa pamalo opingasa ndikulemba chizindikiro.

2.Tengani chitsanzo cha mkodzo ndi pipette yotayika, tayani madontho awiri oyambirira a mkodzo. Onjezani madontho atatu (pafupifupi 100uL) a mkodzo wopanda thovu pakati pa dzenje lachitsanzo la khadi loyeserera molunjika ndikuyamba kuwerengera nthawi.

3.Werengani zotsatira mu mphindi 10-15. Ndizosavomerezeka ngati kupitilira mphindi 15.