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Diagnostic Kit for Total Triiodothyronine(fluorescence immunochromatographic assay)
Kwa in vitro diagnostic ntchito kokha
Chonde werengani phukusili Ikani mosamala musanagwiritse ntchito ndikutsatira malangizowo. Kudalirika kwa zotsatira zoyesa sikungatsimikizidwe ngati pali zolakwika zilizonse kuchokera pamalangizo omwe ali mu phukusili.
ZOFUNIKA KUGWIRITSA NTCHITO
Diagnostic Kit for Total Triiodothyronine (fluorescence immunochromatographic assay) ndi fluorescence immunochromatographic assay pofuna kudziwa kuchuluka kwa Total Triiodothyronine (TT3) mu seramu yaumunthu kapena plasma, yomwe imagwiritsidwa ntchito makamaka kuyesa ntchito ya chithokomiro. ziyenera kutsimikiziridwa ndi njira zina. Kuyezetsa kumeneku kumangogwiritsidwa ntchito ndi akatswiri azachipatala okha.
CHIDULE
Triiodothyronine(T3) molekyulu yolemera 651D. Ndilo gawo lalikulu la mahomoni a chithokomiro. Total T3(Total T3, TT3) mu seramu imagawidwa m'mitundu yomangiriza komanso yaulere. 99.5 % ya TT3 imamanga ku seramu ya Thyroxine Binding Proteins(TBP), ndipo T3 yaulere (T3 yaulere) imakhala 0.2 mpaka 0.4%. T4 ndi T3 zimatenga nawo mbali pakusunga ndi kuwongolera magwiridwe antchito a kagayidwe kachakudya m'thupi.Miyeso ya TT3 imagwiritsidwa ntchito poyesa magwiridwe antchito a chithokomiro komanso kuzindikira matenda. Clinical TT3 ndi chisonyezo chodalirika cha matenda a chithokomiro ndi kuwonetsa bwino kwa hyperthyroidism ndi hypothyroidism.
MFUNDO YA NJIRA
Nembanemba ya chipangizo choyesera imakutidwa ndi cholumikizira cha BSA ndi T3 pagawo loyeserera ndi anti-anti rabbit IgG antibody pachigawo chowongolera. Marker pad amakutidwa ndi fluorescence mark anti T3 antibody ndi kalulu IgG pasadakhale. Mukayesa zitsanzo, TT3 mu zitsanzo imaphatikiza ndi fluorescence yodziwika ndi anti T3 antibody, ndikupanga kusakaniza kwa chitetezo chamthupi. Pansi pa zochita za immunochromatography, kuyenda movutikira kupita ku pepala loyamwa, pamene zovuta zidadutsa gawo loyesa, Chizindikiro cha fulorosenti chaulere chidzaphatikizidwa ndi T3 pa nembanemba. kuchuluka kwa TT3 mu zitsanzo kumatha kudziwika ndi fluorescence immunoassay assay.
REAGENTS NDI Zipangizo ZOPEREKA
Zigawo za phukusi la 25T:
.Khadi loyesera payokha zojambulazo zopakidwa ndi desiccant 25T
.A yankho 25T
.B yankho 1
.Phukusi loyika 1
ZINTHU ZOFUNIKA KOMA ZOSAPATSIDWA
Zitsanzo zosonkhanitsira chidebe, timer
KUSONKHA ZITSANZO NDI KUSINTHA
1.Zitsanzo zoyesedwa zingakhale seramu, heparin anticoagulant plasma kapena EDTA anticoagulant plasma.
2.Malinga ndi njira zoyenera sonkhanitsani zitsanzo. Seramu kapena madzi a m'magazi amatha kusungidwa mufiriji pa 2-8 ℃ kwa 7days ndi cryopreservation pansi -15 ° C kwa miyezi 6.
3.Zitsanzo zonse zimapewa kuzizira kozizira.
NJIRA YOYENERA
Njira yoyesera ya chida onani buku la immunoanalyzer. Njira yoyeserera ya reagent ili motere
1.Ikani pambali ma reagents onse ndi zitsanzo kutentha kutentha.
2.Tsegulani Portable Immune Analyzer (WIZ-A101), lowetsani mawu achinsinsi a akaunti malinga ndi njira yogwiritsira ntchito chida, ndikulowetsani mawonekedwe ozindikira.
3.Scan code dentification kuti mutsimikizire chinthu choyesera.
4.Tulutsani khadi loyesera muthumba la zojambulazo.
5.Lowetsani khadi loyesera mu kagawo ka khadi, jambulani kachidindo ka QR, ndi kuzindikira chinthu choyesera.
6.Onjezani 30μL seramu kapena chitsanzo cha plasma ku A yankho, ndikusakaniza bwino.
7.Onjezani yankho la 20μL B kusakaniza pamwamba, ndikusakaniza bwino.
8.Siyani kusakaniza kwa mphindi 20.
9.Onjezani 80μL osakaniza kuti muyese bwino khadi.
10.Dinani batani la "standard test", pakatha mphindi 10, chidacho chimangozindikira khadi yoyeserera, imatha kuwerenga zotsatira kuchokera pachiwonetsero cha chidacho, ndikulemba / kusindikiza zotsatira zoyeserera.
11. Onani malangizo a Portable Immune Analyzer(WIZ-A101).
MFUNDO ZOYENERA
TT3 yachibadwa osiyanasiyana: 0.5-2.5ng/mL
Ndikoyenera kuti labotale iliyonse ikhazikitse mitundu yakeyake yomwe ikuyimira odwala ake.
ZOTSATIRA ZA MAYESE NDI KUMASULIRA
.Deta yomwe ili pamwambayi ndi nthawi yolozera yomwe yakhazikitsidwa kuti ipeze deta ya zidazi, ndipo akulangizidwa kuti labotale iliyonse iyenera kukhazikitsa nthawi yowonetsera kufunikira kwachipatala kwa anthu m'derali.
.Chiwerengero cha TT3 ndi chapamwamba kuposa chiwerengero chofotokozera, ndipo kusintha kwa thupi kapena kupsinjika maganizo kuyenera kuperekedwa.
.Zotsatira za njirayi zimangogwiritsidwa ntchito pazidziwitso zomwe zakhazikitsidwa ndi njirayi, ndipo zotsatira zake sizingafanane mwachindunji ndi njira zina.
.Zinthu zina zingayambitsenso zolakwika pazotsatira zodziwika, kuphatikizapo zifukwa zamakono, zolakwika zogwirira ntchito ndi zitsanzo zina.
KUSINTHA NDI KUKHALA
1. The zida ndi 18 miyezi alumali moyo kuyambira tsiku kupanga. Sungani zida zosagwiritsidwa ntchito pa 2-30 ° C. OSATI MASIMA. Musagwiritse ntchito kupitirira tsiku lotha ntchito.
2.Musatsegule thumba losindikizidwa mpaka mutakonzeka kuyesa, ndipo kuyesa kamodzi kokha kukuyenera kugwiritsidwa ntchito pansi pa malo ofunikira (kutentha 2-35 ℃, chinyezi 40-90%) mkati mwa mphindi 60 mwamsanga. momwe zingathere.
3.Sample diluent imagwiritsidwa ntchito mwamsanga mutangotsegulidwa.
CHENJEZO NDI CHENJEZO
.Chidacho chiyenera kutsekedwa ndi kutetezedwa ku chinyezi.
.Zitsanzo zonse zabwino zidzatsimikiziridwa ndi njira zina.
.Zitsanzo zonse ziyenera kuonedwa ngati zowononga.
.MUSAMAGWIRITSE NTCHITO zomwe zidatha.
.OSATI kusinthanitsa ma reagents pakati pa zida zomwe zili ndi manambala osiyanasiyana.
.MUSAMAGWIRITSE NTCHITO makhadi oyesera ndi zina zilizonse zomwe zingathe kutayidwa.
.Misoperation, mochulukira kapena pang'ono zitsanzo kungayambitse zotsatira zopotoka.
LKUTSANZITSA
.Monga ndi kuyesa kulikonse komwe kumagwiritsa ntchito ma antibodies a mbewa, kuthekera kulipo kuti kusokonezedwe ndi ma antibodies a anti-mbewa (HAMA) pachitsanzo. Zitsanzo za odwala omwe alandira kukonzekera kwa ma antibodies a monoclonal kuti adziwe matenda kapena mankhwala angakhale ndi HAMA. Zitsanzo zoterezi zingayambitse zotsatira zabodza kapena zabodza.
.Chotsatira choyezetsa ichi ndi chachipatala chokha, sichiyenera kukhala maziko okhawo a matenda ndi chithandizo chamankhwala, chithandizo chamankhwala cha odwala chiyenera kuganiziridwa mozama pamodzi ndi zizindikiro zake, mbiri yachipatala, kufufuza kwa labotale, kuyankha kwamankhwala, miliri ndi zina. .
.Reagent iyi imagwiritsidwa ntchito poyezetsa seramu ndi plasma. Ikhoza kusapeza zotsatira zolondola zikagwiritsidwa ntchito pa zitsanzo zina monga malovu ndi mkodzo ndi zina.
ZINTHU ZOCHITIKA
Linearity | 0.25 ng/mL mpaka 10 ng/mL | Kupatuka kwachibale: -15% mpaka +15%. |
Linear coefficient:(r)≥0.9900 | ||
Kulondola | Mlingo wochira uyenera kukhala mkati mwa 85% - 115%. | |
Kubwerezabwereza | CV≤15% | |
Mwatsatanetsatane(Palibe zinthu zomwe zidayesedwa zomwe zidasokoneza mayesowo) | Wosokoneza | Kusokoneza maganizo |
Hemoglobin | 200μg/mL | |
transferrin | 100μg/mL | |
Horseradish Peroxidase | 2000μg/mL | |
rT3 ndi | 100ng/mL | |
T4 | 200ng/mL |
RMALANGIZO
1.Hansen JH, ndi al.HAMA Kusokoneza ndi Murine Monoclonal Antibody-Based Immunoassays [J].J ya Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Chilengedwe cha Heterophilic Antibodies ndi Udindo mu Immunoassay Interference[J].J wa Clin Immunoassay,1992,15:108-114.
Chinsinsi cha zizindikiro zogwiritsidwa ntchito:
In Vitro Diagnostic Medical Chipangizo | |
Wopanga | |
Sungani pa 2-30 ℃ | |
Tsiku lothera ntchito | |
Osagwiritsanso Ntchito | |
CHENJEZO | |
Funsani Malangizo Ogwiritsa Ntchito |
Xiamen Wiz Biotech CO., LTD
Address:3-4 Floor,NO.16 Building,Bio-medical Workshop,2030 Wengjiao West Road,Haicang District,361026,Xiamen,China
Tel: + 86-592-6808278
Fax: + 86-592-6808279