MFUNDO

Nembanemba ya chipangizo choyesera imakutidwa ndi antibody ya FOB pamalo oyeserera ndi anti-Rabbit IgG antibody pagawo lowongolera. Lable pad amakutidwa ndi fluorescence otchedwa anti FOB antibody ndi kalulu IgG pasadakhale. Mukayesa zitsanzo zabwino, antigen ya FOB mu zitsanzo imaphatikizana ndi fluorescence yotchedwa anti FOB antibody, ndikupanga kusakaniza kwa chitetezo cha mthupi. Pansi pa zochita za immunochromatography, zovuta kuyenda mu malangizo a absorbent pepala. Zovuta zikadutsa gawo loyesa, zimaphatikizidwa ndi anti FOB zokutira antibody, zimapanga zovuta zatsopano. Ngati zili zoipa, chitsanzocho chilibe hemoglobini yaumunthu kapena zomwe zili ndi zochepa, chitetezo chokwanira chokwanira sichingapangidwe. Sipadzakhala mzere wofiira pamalo ozindikira (T). Mzere wofiyira ndi muyezo womwe umawonekera m'dera lowongolera (C) kuti muwone ngati pali zitsanzo zokwanira komanso ngati njira ya chromatography ndi yabwinobwino. Amagwiritsidwanso ntchito ngati muyezo wowongolera mkati mwa ma reagents.

Njira Yoyesera:

Chonde werengani phukusili musanayese.

1.Tulutsani khadi loyesera kuchokera mu thumba la zojambulazo, liyikeni pa tebulo lapamwamba ndikuyika chizindikiro.

2.Chotsani kapu kuchokera ku chubu lachitsanzo ndikutaya madontho awiri oyambirira osungunuka, onjezerani madontho a 3 (pafupifupi 100uL) palibe kuwira kuchepetsedwa chitsanzo vertically ndi pang'onopang'ono mu chitsanzo chitsime cha khadi ndi dispette anapereka. Kenako yambani chowerengera.

3.Zotsatira ziyenera kuwerengedwa mkati mwa mphindi 10-15, ndipo ndizosavomerezeka pakatha mphindi 15.