Zida Zowunikira za Total Thyroxine (fluorescence immunochromatographic assay)
Kwa in vitro diagnostic ntchito kokha
Chonde werengani phukusili Ikani mosamala musanagwiritse ntchito ndikutsatira malangizowo. Kudalirika kwa zotsatira zoyesa sikungatsimikizidwe ngati pali zolakwika zilizonse kuchokera pamalangizo omwe ali mu phukusili.

ZOFUNIKA KUGWIRITSA NTCHITO
Diagnostic Kit for Total Thyroxine (fluorescence immunochromatographic assay) ndi fluorescence immunochromatographic assay pakuzindikira kuchuluka kwa Total Thyroxine (TT4) mu seramu yamunthu kapena plasma, yomwe imagwiritsidwa ntchito makamaka kuyesa ntchito ya chithokomiro. ziyenera kutsimikiziridwa ndi njira zina. Kuyezetsa kumeneku kumangogwiritsidwa ntchito ndi akatswiri azachipatala okha.

CHIDULE
Thyroxine (T4) imatulutsidwa ndi chithokomiro ndipo kulemera kwake ndi 777D. T4 (Total T4,TT4) yonse mu seramu ndi nthawi 50 kuposa ya seramu T3. Pakati pawo, 99.9 % ya TT4 imamangiriza ku seramu ya Thyroxine Binding Proteins(TBP), ndipo T4 yaulere (Free T4,FT4) ndi yochepera 0.05%. T4 ndi T3 amatenga nawo gawo pakuwongolera kagayidwe kachakudya m'thupi. Miyezo ya TT4 imagwiritsidwa ntchito poyesa momwe chithokomiro chimagwirira ntchito komanso kuzindikira matenda. Zachipatala, TT4 ndi chizindikiro chodalirika cha matenda a chithokomiro ndi kuwonetsetsa kwapamwamba kwa hyperthyroidism ndi hypothyroidism.

MFUNDO YA NJIRA
Nembanemba ya chipangizo choyesera imakutidwa ndi conjugate ya BSA ndi T4 pagawo loyesa ndi anti-Rabbit IgG antibody pachigawo chowongolera. Marker pad amakutidwa ndi fluorescence mark anti T4 antibody ndi kalulu IgG pasadakhale. Mukayesa zitsanzo, TT4 mu zitsanzo imaphatikizana ndi fluorescence yodziwika ndi anti T4 antibody, ndikupanga kusakaniza kwa chitetezo chamthupi. Pansi pa zochita za immunochromatography, zovuta kuyenda molunjika ku mbali ya kuyamwa pepala, pamene zovuta zadutsa dera loyesa, Chizindikiro cha fulorosenti yaulere chidzaphatikizidwa ndi T4 pa nembanemba. Kuchuluka kwa TT4 mu zitsanzo kumatha kudziwika ndi fluorescence immunoassay assay.

REAGENTS NDI Zipangizo ZOPEREKA

Zigawo za phukusi la 25T:
.Khadi loyesera payokha zojambulazo zopakidwa ndi desiccant 25T
.A yankho 25T
.B yankho 1
.Phukusi loyika 1

ZINTHU ZOFUNIKA KOMA ZOSAPATSIDWA
Zitsanzo zosonkhanitsira chidebe, timer

KUSONKHA ZITSANZO NDI KUSINTHA
1.Zitsanzo zoyesedwa zingakhale seramu, heparin anticoagulant plasma kapena EDTA anticoagulant plasma.
2.Malinga ndi njira zoyenera sonkhanitsani zitsanzo. Seramu kapena madzi a m'magazi amatha kusungidwa mufiriji pa 2-8 ℃ kwa 7days ndi cryopreservation pansi -15 ° C kwa miyezi 6.
3.Zitsanzo zonse zimapewa kuzizira kozizira.

NJIRA YOYENERA

Njira yoyesera ya chida onani buku la immunoanalyzer. Njira yoyeserera ya reagent ili motere
1.Ikani pambali ma reagents onse ndi zitsanzo kutentha kutentha.
2.Tsegulani Portable Immune Analyzer (WIZ-A101), lowetsani mawu achinsinsi a akaunti malinga ndi njira yogwiritsira ntchito chida, ndikulowetsani mawonekedwe ozindikira.
3.Scan code dentification kuti mutsimikizire chinthu choyesera.
3.Tulutsani khadi loyesera muthumba la zojambulazo.
4.Lowetsani khadi loyesera mu kagawo ka khadi, jambulani kachidindo ka QR, ndi kuzindikira chinthu choyesera.
5.Onjezani 20μL seramu kapena chitsanzo cha plasma ku A yankho, ndikusakaniza bwino.
6.Onjezani yankho la 20μL B kusakaniza pamwamba, ndikusakaniza bwino.

Siyani kusakaniza kwa20mphindi.
Onjezani kusakaniza kwa 80μL kuti muyese bwino khadi.
Dinani batani la "standard test", pakatha mphindi 10, chidacho chimangozindikira khadi yoyeserera, imatha kuwerenga zotsatira kuchokera pachiwonetsero cha chidacho, ndikulemba / kusindikiza zotsatira zoyeserera.
Onani malangizo a Portable Immune Analyzer(WIZ-A101).

MFUNDO ZOYENERA
TT4 wamba: 55-140nmol / L
Ndikoyenera kuti labotale iliyonse ikhazikitse mitundu yakeyake yomwe ikuyimira odwala ake.

ZOTSATIRA ZA MAYESE NDI KUMASULIRA
.Deta yomwe ili pamwambayi ndi nthawi yolozera yomwe yakhazikitsidwa kuti ipeze deta ya zidazi, ndipo akulangizidwa kuti labotale iliyonse iyenera kukhazikitsa nthawi yowonetsera kufunikira kwachipatala kwa anthu m'derali.
.Chiwerengero cha TT4 ndipamwamba kuposa chiwerengero chofotokozera, ndipo kusintha kwa thupi kapena kupsinjika maganizo kuyenera kuperekedwa.
.Zotsatira za njirayi zimangogwiritsidwa ntchito pazidziwitso zomwe zakhazikitsidwa ndi njirayi, ndipo zotsatira zake sizingafanane mwachindunji ndi njira zina.
.Zinthu zina zingayambitsenso zolakwika pazotsatira zodziwika, kuphatikizapo zifukwa zamakono, zolakwika zogwirira ntchito ndi zitsanzo zina.

KUSINTHA NDI KUKHALA
.Zidazi ndi miyezi 18 ya alumali kuyambira tsiku lopangidwa. Sungani zida zosagwiritsidwa ntchito pa 2-30 ° C. OSATI MASIMA. Osagwiritsa ntchito kupitirira tsiku lotha ntchito.
.Musatsegule thumba losindikizidwa mpaka mutakonzeka kuyesa, ndipo kuyesa kamodzi kokha kumalangizidwa kuti agwiritsidwe ntchito pansi pa malo ofunikira (kutentha 2-35 ℃, chinyezi 40-90%) mkati mwa mphindi 60 mwamsanga zotheka.
.Sample diluent amagwiritsidwa ntchito atangotsegulidwa.

CHENJEZO NDI CHENJEZO
.Chidacho chiyenera kutsekedwa ndi kutetezedwa ku chinyezi.
.Zitsanzo zonse zabwino zidzatsimikiziridwa ndi njira zina.
.Zitsanzo zonse ziyenera kuonedwa ngati zowononga.
.MUSAMAGWIRITSE NTCHITO zomwe zidatha.
.OSATI kusinthanitsa ma reagents pakati pa zida zomwe zili ndi manambala osiyanasiyana.
.MUSAMAGWIRITSE NTCHITO makhadi oyesera ndi zina zilizonse zomwe zingathe kutayidwa.
.Misoperation, mochulukira kapena pang'ono zitsanzo kungayambitse zotsatira zopotoka.

LKUTSANZITSA
.Monga ndi kuyesa kulikonse komwe kumagwiritsa ntchito ma antibodies a mbewa, kuthekera kulipo kuti kusokonezedwe ndi ma antibodies a anti-mbewa (HAMA) pachitsanzo. Zitsanzo za odwala omwe alandira kukonzekera kwa ma antibodies a monoclonal kuti adziwe matenda kapena mankhwala angakhale ndi HAMA. Zitsanzo zoterezi zingayambitse zotsatira zabodza kapena zabodza.
.Chotsatira choyezetsa ichi ndi chachipatala chokha, sichiyenera kukhala maziko okhawo a matenda ndi chithandizo chamankhwala, chithandizo chamankhwala cha odwala chiyenera kuganiziridwa mozama pamodzi ndi zizindikiro zake, mbiri yachipatala, kufufuza kwa labotale, kuyankha kwamankhwala, miliri ndi zina. .
.Reagent iyi imagwiritsidwa ntchito poyezetsa seramu ndi plasma. Ikhoza kusapeza zotsatira zolondola zikagwiritsidwa ntchito pa zitsanzo zina monga malovu ndi mkodzo ndi zina.

ZINTHU ZOCHITIKA

RMALANGIZO
1.Hansen JH, ndi al.HAMA Kusokoneza ndi Murine Monoclonal Antibody-Based Immunoassays [J].J ya Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Chilengedwe cha Heterophilic Antibodies ndi Udindo mu Immunoassay Interference[J].J wa Clin Immunoassay,1992,15:108-114.

Chinsinsi cha zizindikiro zogwiritsidwa ntchito:

	In Vitro Diagnostic Medical Chipangizo
	Wopanga
	Sungani pa 2-30 ℃
	Tsiku lothera ntchito
	Osagwiritsanso Ntchito
	CHENJEZO
	Funsani Malangizo Ogwiritsa Ntchito

Xiamen Wiz Biotech CO., LTD
Address:3-4 Floor,NO.16 Building,Bio-medical Workshop,2030 Wengjiao West Road,Haicang District,361026,Xiamen,China
Tel: + 86-592-6808278
Fax: + 86-592-6808279