Kit Diagnostic for Luteinizing Hormone (fluorescence immunochromatographic assay)
Diagnostic Kit kwaLuteinizing Hormonefluorescence immunochromatographic assay)
Kwa in vitro diagnostic ntchito kokha
Chonde werengani phukusili Ikani mosamala musanagwiritse ntchito ndikutsatira malangizowo. Kudalirika kwa zotsatira zoyesa sikungatsimikizidwe ngati pali zolakwika zilizonse kuchokera pamalangizo omwe ali mu phukusili.
ZOFUNIKA KUGWIRITSA NTCHITO
Diagnostic Kit for Luteinizing Hormone (fluorescence immunochromatographic assay) ndi fluorescence immunochromatographic assay kwa kuchuluka kwa Luteinizing Hormone (LH) mu seramu yamunthu kapena plasma, yomwe imagwiritsidwa ntchito makamaka pakuwunika kwa pituitary endocrine ntchito. njira zina. Kuyezetsa kumeneku kumangogwiritsidwa ntchito ndi akatswiri azachipatala okha.
CHIDULE
Luteinizing hormone (LH) ndi glycoprotein yokhala ndi molekyulu yolemera pafupifupi 30,000 Dalton, yomwe imapangidwa ndi anterior pituitary. Kuchuluka kwa LH kumakhudzana kwambiri ndi kutulutsa mazira, ndipo nsonga ya LH imanenedweratu kuti idzakhala maola 24 mpaka 36 a ovulation. Choncho, mtengo wapamwamba wa LH ukhoza kuyang'aniridwa panthawi ya msambo kuti mudziwe nthawi yoyenera kutenga pakati. Kusakhazikika kwa endocrine mu chithokomiro cha pituitary kungayambitse LH secretion irregularity.Kuchuluka kwa LH kungagwiritsidwe ntchito poyesa ntchito ya pituitary endocrine. The Diagnostic Kit imachokera ku immunochromatography ndipo imatha kupereka zotsatira mkati mwa mphindi 15.
MFUNDO YA NJIRA
Nembanemba ya chipangizo choyesera imakutidwa ndi anti-LH antibody pagawo loyesa ndi anti-Rabbit IgG antibody pagawo lowongolera. Lable pad amakutidwa ndi fluorescence otchedwa anti LH antibody ndi kalulu IgG pasadakhale. Mukayesa zitsanzo zabwino, antigen ya LH mu zitsanzo imaphatikizana ndi fluorescence yotchedwa anti LH antibody, ndikupanga kusakaniza kwa chitetezo chamthupi. Pansi pa zochita za immunochromatography, zovuta kuyenda molunjika ku mbali ya kuyamwa pepala, pamene zovuta zadutsa dera loyesa, zimaphatikizidwa ndi anti-LH zokutira antibody, zimapanga zovuta zatsopano. Mulingo wa LH umalumikizidwa bwino ndi siginecha ya fluorescence, ndipo kuchuluka kwa LH mu zitsanzo kumatha kudziwika ndi fluorescence immunoassay assay.
REAGENTS NDI Zipangizo ZOPEREKA
Zigawo za phukusi la 25T:
.Khadi loyesera payokha zojambulazo zopakidwa ndi desiccant 25T
.Zitsanzo za diluent
.Kuyika phukusi
ZINTHU ZOFUNIKA KOMA ZOSAPATSIDWA
Zitsanzo zosonkhanitsira chidebe, timer
KUSONKHA ZITSANZO NDI KUSINTHA
1.Zitsanzo zoyesedwa zingakhale seramu, heparin anticoagulant plasma kapena EDTA anticoagulant plasma.
2.Malinga ndi njira zoyenera sonkhanitsani zitsanzo. Seramu kapena madzi a m'magazi amatha kusungidwa mufiriji pa 2-8 ℃ kwa 7days ndi cryopreservation pansi -15 ° C kwa miyezi 6.
3.Zitsanzo zonse zimapewa kuzizira kozizira.
NJIRA YOYENERA
Njira yoyesera ya chida onani buku la immunoanalyzer. Njira yoyeserera ya reagent ili motere
1.Ikani pambali ma reagents onse ndi zitsanzo kutentha kutentha.
2.Tsegulani Portable Immune Analyzer (WIZ-A101), lowetsani mawu achinsinsi a akaunti malinga ndi njira yogwiritsira ntchito chida, ndikulowetsani mawonekedwe ozindikira.
3.Scan code dentification kuti mutsimikizire chinthu choyesera.
4.Tulutsani khadi loyesera muthumba la zojambulazo.
5.Lowetsani khadi loyesera mu kagawo ka khadi, jambulani kachidindo ka QR, ndi kuzindikira chinthu choyesera.
6.Add 20μL seramu kapena madzi a m'magazi zitsanzo diluent, ndi kusakaniza bwino.
7.Onjezani 80μL yachitsanzo chachitsulo kuti muyese bwino khadi.
8.Dinani batani la "standard test", pakatha mphindi 15, chidacho chidzangozindikira khadi yoyesera, imatha kuwerenga zotsatira kuchokera pachiwonetsero chowonetsera chida, ndikulemba / kusindikiza zotsatira zoyesa.
9. Onani malangizo a Portable Immune Analyzer(WIZ-A101).
ZOTSATIRA ZA MAYESE NDI KUMASULIRA
Gawo | Range(mIU/mL) | |
Mwamuna | 1.50-9.25 | |
Mkazi | gawo la follicular | 1.25-11.80 |
| nthawi ya ovulatory | 13.15-94.75 |
| Gawo la Luteal | 1.05-14.50 |
| Kusiya kusamba | 7.70-64.20 |
.Deta yomwe ili pamwambayi ndi nthawi yolozera yomwe yakhazikitsidwa kuti ipeze deta ya zidazi, ndipo akulangizidwa kuti labotale iliyonse iyenera kukhazikitsa nthawi yowonetsera kufunikira kwachipatala kwa anthu m'derali.
.Chiwerengero cha LH ndipamwamba kuposa chiwerengero chofotokozera, ndipo kusintha kwa thupi kapena kupsinjika maganizo kuyenera kuperekedwa.
.Zotsatira za njirayi zimangogwiritsidwa ntchito pazidziwitso zomwe zakhazikitsidwa ndi njirayi, ndipo zotsatira zake sizingafanane mwachindunji ndi njira zina.
.Zinthu zina zingayambitsenso zolakwika pazotsatira zodziwika, kuphatikizapo zifukwa zamakono, zolakwika zogwirira ntchito ndi zitsanzo zina.
KUSINTHA NDI KUKHALA
1. The zida ndi 18 miyezi alumali moyo kuyambira tsiku kupanga. Sungani zida zosagwiritsidwa ntchito pa 2-30 ° C. OSATI MASIMA. Osagwiritsa ntchito kupitirira tsiku lotha ntchito.
2.Musatsegule thumba losindikizidwa mpaka mutakonzeka kuyesa, ndipo kuyesa kamodzi kokha kukuyenera kugwiritsidwa ntchito pansi pa malo ofunikira (kutentha 2-35 ℃, chinyezi 40-90%) mkati mwa mphindi 60 mwamsanga. momwe zingathere.
3.Sample diluent imagwiritsidwa ntchito mwamsanga mutangotsegulidwa.
CHENJEZO NDI CHENJEZO
.Chidacho chiyenera kutsekedwa ndi kutetezedwa ku chinyezi.
.Zitsanzo zonse zabwino zidzatsimikiziridwa ndi njira zina.
.Zitsanzo zonse ziyenera kuonedwa ngati zowononga.
.MUSAMAGWIRITSE NTCHITO zomwe zidatha.
. OSATI osinthana ma reagents pakati pa zida zokhala ndi magawo osiyanasiyana No.
.MUSAMAGWIRITSE NTCHITO makhadi oyesera ndi zina zilizonse zomwe zingathe kutayidwa.
.Misoperation, mochulukira kapena pang'ono zitsanzo kungayambitse zotsatira zopotoka.
LKUTSANZITSA
.Monga ndi kuyesa kulikonse komwe kumagwiritsa ntchito ma antibodies a mbewa, kuthekera kulipo kuti kusokonezedwe ndi ma antibodies a anti-mbewa (HAMA) pachitsanzo. Zitsanzo za odwala omwe alandira kukonzekera kwa ma antibodies a monoclonal kuti adziwe matenda kapena mankhwala angakhale ndi HAMA. Zitsanzo zoterezi zingayambitse zotsatira zabodza kapena zabodza.
.Chotsatira choyezetsa ichi ndi chachipatala chokha, sichiyenera kukhala maziko okhawo a matenda ndi chithandizo chamankhwala, chithandizo chamankhwala cha odwala chiyenera kuganiziridwa mozama pamodzi ndi zizindikiro zake, mbiri yachipatala, kufufuza kwa labotale, kuyankha kwamankhwala, miliri ndi zina. .
.Reagent iyi imagwiritsidwa ntchito poyezetsa seramu ndi plasma. Ikhoza kusapeza zotsatira zolondola zikagwiritsidwa ntchito pa zitsanzo zina monga malovu ndi mkodzo ndi zina.
ZINTHU ZOCHITIKA
Linearity | 10mIU/mL mpaka 10000mIU/mL | Kupatuka kwachibale: -15% mpaka +15%. |
Linear coefficient:(r)≥0.9900 | ||
Kulondola | Mlingo wochira uyenera kukhala mkati mwa 85% - 115%. | |
Kubwerezabwereza | CV≤15% | |
Kufotokozera (Palibe zinthu zomwe zidayesedwa zomwe zidasokoneza kuyesa) | Wosokoneza | Kusokoneza maganizo |
Hemoglobin | 200μg/mL | |
transferrin | 100μg/mL | |
Horse radish peroxidase | 2000μg/mL | |
Mtengo wa FSH | 200mIU/mL | |
HCG | 20000mIU/mL | |
TSH | 200μIU/mL |
MALONJE
1.Hansen JH, ndi al.HAMA Kusokoneza ndi Murine Monoclonal Antibody-Based Immunoassays [J].J ya Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Chilengedwe cha Heterophilic Antibodies ndi Udindo mu Immunoassay Interference[J].J wa Clin Immunoassay,1992,15:108-114.
Chinsinsi cha zizindikiro zogwiritsidwa ntchito:
In Vitro Diagnostic Medical Chipangizo | |
Wopanga | |
Sungani pa 2-30 ℃ | |
Tsiku lothera ntchito | |
Osagwiritsanso Ntchito | |
CHENJEZO | |
Funsani Malangizo Ogwiritsa Ntchito |
Xiamen Wiz Biotech CO., LTD
Address:3-4 Floor,NO.16 Building,Bio-medical Workshop,2030 Wengjiao West Road,Haicang District,361026,Xiamen,China
Tel: + 86-592-6808278
Fax: + 86-592-6808279