Diagnostic Kit for Isoenzyme MB ya Creatine Kinase(fluorescence immunochromatographic assay)
Diagnostic Kit ya Isoenzyme MB ya Creatine Kinase(fluorescence immunochromatographic assay)
Kwa in vitro diagnostic ntchito kokha
Chonde werengani phukusili Ikani mosamala musanagwiritse ntchito ndikutsatira malangizowo. Kudalirika kwa zotsatira zoyesa sikungatsimikizidwe ngati pali zolakwika zilizonse kuchokera pamalangizo omwe ali mu phukusili.
ZOFUNIKA KUGWIRITSA NTCHITO
Diagnostic Kit for Isoenzyme MB ya Creatine Kinase(fluorescence immunochromatographic assay) is a fluorescence immunochromatographic assay for the quantitative discovery of Isoenzyme MB ya Creatine Kinase (CK-MB) mu seramu yamunthu kapena plasma, imagwiritsidwa ntchito pozindikira matenda a AMI (Acute Mcardial) Infarction). Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina. Kuyezetsa kumeneku kumangogwiritsidwa ntchito ndi akatswiri azachipatala okha.
CHIDULE
Miyezo ya CK-MB idakwera maola 4-6 pambuyo pa infarction ya Myocardial, idakwera kwambiri maola 18-24, ndikubwereranso masiku 2-3 pambuyo pake. CK-MB kutulutsidwa m'magazi mochedwa kuposa hemoglobin.CK-MB ndiye chizindikiro chachikhalidwe cha acute myocardial infarction.
MFUNDO YA NJIRA
Nembanemba ya chipangizo choyesera imakutidwa ndi anti CK-MB antibody pagawo loyesa ndi anti-Rabbit IgG antibody pachigawo chowongolera. Padi lable amakutidwa ndi fluorescence olembedwa anti CK-MB antibody ndi kalulu IgG pasadakhale. Poyesa zitsanzo zabwino, antigen ya CK-MB mu zitsanzo imaphatikizana ndi fluorescence yotchedwa anti CK-MB antibody, ndikupanga kusakaniza kwa chitetezo cha mthupi. Pansi pa zochita za immunochromatography, kuyenda movutikira kumayendedwe a pepala loyamwa, pamene zovuta zidadutsa dera loyesa, zimaphatikizidwa ndi anti-CK-MB zokutira antibody, zimapanga zovuta zatsopano. Mulingo wa CK-MB umalumikizidwa bwino ndi siginecha ya fluorescence, ndipo kuchuluka kwa CK-MB pachitsanzo kumatha kudziwika ndi fluorescence immunoassay assay.
REAGENTS NDI Zipangizo ZOPEREKA
Zigawo za phukusi la 25T:
Khadi yoyesera payokha zojambulazo zopakidwa ndi desiccant 25T
Zitsanzo za diluent 25T
Phukusi 1
ZINTHU ZOFUNIKA KOMA ZOSAPATSIDWA
Zitsanzo zosonkhanitsira chidebe, timer
KUSONKHA ZITSANZO NDI KUSINTHA
1.Zitsanzo zoyesedwa zingakhale seramu, heparin anticoagulant plasma kapena EDTA anticoagulant plasma.
2.Malinga ndi njira zoyenera sonkhanitsani zitsanzo. Seramu kapena madzi a m'magazi amatha kusungidwa mufiriji pa 2-8 ℃ kwa 7days ndi cryopreservation pansi -15 ° C kwa miyezi 6.
3.Zitsanzo zonse zimapewa kuzizira kozizira.
NJIRA YOYENERA
Chonde werengani buku lothandizira zida ndi kuyika phukusi musanayese.
1.Ikani pambali ma reagents onse ndi zitsanzo kutentha kutentha.
2.Tsegulani Portable Immune Analyzer (WIZ-A101), lowetsani mawu achinsinsi a akaunti malinga ndi njira yogwiritsira ntchito chida, ndikulowetsani mawonekedwe ozindikira.
3.Scan code dentification kuti mutsimikizire chinthu choyesera.
4.Tulutsani khadi loyesera muthumba la zojambulazo.
5.Lowetsani khadi loyesera mu kagawo ka khadi, jambulani kachidindo ka QR, ndi kuzindikira chinthu choyesera.
5.Add 40μL seramu kapena madzi a m'magazi zitsanzo diluent, ndi kusakaniza bwino.
6.Onjezani 80μL yachitsanzo chachitsulo kuti muyese bwino khadi.
7.Dinani batani la "standard test", pakatha mphindi 15, chidacho chidzangozindikira khadi yoyesera, imatha kuwerenga zotsatira kuchokera pachiwonetsero chowonetsera chida, ndikulemba / kusindikiza zotsatira zoyesa.
8. Onani malangizo a Portable Immune Analyzer(WIZ-A101).
MFUNDO ZOYENERA
CK-MB <5ng/mL
Ndikoyenera kuti labotale iliyonse ikhazikitse mitundu yakeyake yomwe ikuyimira odwala ake.
ZOTSATIRA ZA MAYESE NDI KUMASULIRA
.Deta yomwe ili pamwambayi ndi zotsatira za kuyesa kwa CK-MB reagent, ndipo akulangizidwa kuti labotale iliyonse iyenera kukhazikitsa miyeso yodziwika bwino ya CK-MB yoyenera anthu okhala m'derali. Zotsatira zomwe zili pamwambazi ndizongowona.
.Zotsatira za njirayi zimangogwiritsidwa ntchito pazigawo zowonetsera zomwe zakhazikitsidwa mu njira iyi, ndipo palibe kufananizidwa kwachindunji ndi njira zina.
.Zinthu zina zingayambitsenso zolakwika pazotsatira zodziwika, kuphatikizapo zifukwa zamakono, zolakwika zogwirira ntchito ndi zitsanzo zina.
KUSINTHA NDI KUKHALA
1. The zida ndi 18 miyezi alumali moyo kuyambira tsiku kupanga. Sungani zida zosagwiritsidwa ntchito pa 2-30 ° C. OSATI MASIMA. Osagwiritsa ntchito kupitirira tsiku lotha ntchito.
2.Musatsegule thumba losindikizidwa mpaka mutakonzeka kuyesa, ndipo kuyesa kamodzi kokha kukuyenera kugwiritsidwa ntchito pansi pa malo ofunikira (kutentha 2-35 ℃, chinyezi 40-90%) mkati mwa mphindi 60 mwamsanga. momwe zingathere.
3.Sample diluent imagwiritsidwa ntchito mwamsanga mutangotsegulidwa.
CHENJEZO NDI CHENJEZO
.Chidacho chiyenera kutsekedwa ndi kutetezedwa ku chinyezi.
.Zitsanzo zonse zabwino zidzatsimikiziridwa ndi njira zina.
.Zitsanzo zonse ziyenera kuonedwa ngati zowononga.
.MUSAMAGWIRITSE NTCHITO zomwe zidatha.
.OSATANA kusinthanitsa ma reagents pakati pa zida zomwe zili ndi magawo osiyanasiyana No.
.MUSAMAGWIRITSE NTCHITO makhadi oyesera ndi zina zilizonse zomwe zingathe kutayidwa.
.Misoperation, mochulukira kapena pang'ono zitsanzo kungayambitse zotsatira zopotoka.
LKUTSANZITSA
.Monga ndi kuyesa kulikonse komwe kumagwiritsa ntchito ma antibodies a mbewa, kuthekera kulipo kuti kusokonezedwe ndi ma antibodies a anti-mbewa (HAMA) pachitsanzo. Zitsanzo za odwala omwe alandira kukonzekera kwa ma antibodies a monoclonal kuti adziwe matenda kapena mankhwala angakhale ndi HAMA. Zitsanzo zoterezi zingayambitse zotsatira zabodza kapena zabodza.
.Chotsatira choyezetsa ichi ndi chachipatala chokha, sichiyenera kukhala maziko okhawo a matenda ndi chithandizo chamankhwala, chithandizo chamankhwala cha odwala chiyenera kuganiziridwa mozama pamodzi ndi zizindikiro zake, mbiri yachipatala, kufufuza kwa labotale, kuyankha kwamankhwala, miliri ndi zina. .
.Reagent iyi imagwiritsidwa ntchito poyezetsa seramu ndi plasma. Ikhoza kusapeza zotsatira zolondola zikagwiritsidwa ntchito pa zitsanzo zina monga malovu ndi mkodzo ndi zina.
ZINTHU ZOCHITIKA
Linearity | 0.5ng/mL mpaka 80ng/mL | Kupatuka kwachibale: -15% mpaka +15%. |
Linear coefficient:(r)≥0.9900 | ||
Kulondola | Mlingo wochira uyenera kukhala mkati mwa 85% - 115%. | |
Kubwerezabwereza | CV≤15% | |
Mwatsatanetsatane(Palibe zinthu zomwe zidayesedwa zomwe zidasokoneza mayesowo) | Wosokoneza | Kusokoneza maganizo |
sTnI | 1000μg/L | |
cTnT | 1000μg/L | |
ABP | 1000μg/L | |
cTnI | 1000μg/L | |
cTnC | 1000μg/L | |
sTnT | 1000μg/L | |
MYO | 1000μg/L |
RMALANGIZO
1.Hansen JH, ndi al.HAMA Kusokoneza ndi Murine Monoclonal Antibody-Based Immunoassays [J].J ya Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Chilengedwe cha Heterophilic Antibodies ndi Udindo mu Immunoassay Interference[J].J wa Clin Immunoassay,1992,15:108-114.
Chinsinsi cha zizindikiro zogwiritsidwa ntchito:
In Vitro Diagnostic Medical Chipangizo | |
Wopanga | |
Sungani pa 2-30 ℃ | |
Tsiku lothera ntchito | |
Osagwiritsanso Ntchito | |
CHENJEZO | |
Funsani Malangizo Ogwiritsa Ntchito |
Xiamen Wiz Biotech CO., LTD
Address:3-4 Floor,NO.16 Building,Bio-medical Workshop,2030 Wengjiao West Road,Haicang District,361026,Xiamen,China
Tel: + 86-592-6808278
Fax: + 86-592-6808279