Chifukwa mu diacro wozindikira okha

Chonde werengani phukusi ili musanagwiritsidwe ntchito ndikutsatira mwachikondi malangizowo. Kudalirika kwa zotsatira za Astay sikungatsimikizidwe ngati pali zopatuka pazinthu zomwe zili mu phukusi ili.

Kugwiritsa Ntchito

Matenda a diatititis c virus antibody (ma flurescence immunoromatographic chizindikiritso cha HCV mu seramu) njira. Kuyesaku kumapangidwira ntchito zaumoyo wathanzi kokha

1.Tikweza ma reagents onse ndi zitsanzo kutentha.
2.Pen Worser Worlint (Wiz-A101), Lowetsani Chinsinsi cha Akaunti Malinga ndi Njira Yogwirira Ntchitoyo, ndikulowetsa mawonekedwe.
3.Scan nambala ya decotion kuti itsimikizire chinthucho.
4.Tukani khadi yoyeserera kuchokera m'thumba la foil.
5. Onani khadi yoyeserera mu khadi la khadi, jambulani nambala ya QR, ndikuwonetsa chinthucho.
6.Add 20μl seramu kapena plasma zitsanzo kuti ayambe kuzisamalira, ndikusakaniza bwino ..
7.dd 80μl zitsanzo zothetsera chiwonetsero cha khadi.
8.CLLick "Medial Kuyesa", patatha mphindi 15, chida chimatha kudziwa khadi yoyeserera, imatha kuwerenga zotsatira kuchokera pazenera la chipangizocho, ndikulemba / kusindikiza zotsatira zake.
9.Lemiyer ku malangizo a wopenda wosambira (Wiz-A101).

Chidule

Hepatitis C (hcv) ndi envulopu, imodzi yokha yomwe ili ndi kachilombo ka RNA (9,5 KB) ya banja la Flaviviridae. Asanu ndi umodzi akuluakulu ndi mndandanda wa babypes wa HCV yadziwika. Kutalikirana mu 1989, HCV tsopano imadziwika kuti ndi chifukwa chachikulu chogwirira ntchito chosagwirizana ndi anthu, osakhala a Bhepatitis. Matendawa amadziwika ndi mawonekedwe komanso osavuta. Oposa 50% ya anthu omwe ali ndi kachilombo amakulira kwambiri hepatitis matenda a chiwindi a chiwindi ndi hepatocellum carcinomas. Kuyambira koyamba mu 1990 ya kuwunika kwa zopereka zamagazi, zomwe zimachitika chifukwa cha matendawa omwe amathandizidwa ndi magazi achepetsedwa kwambiri. Kafukufuku wazachipatala akuwonetsa kuti anthu omwe ali ndi kachilombo ka HCV omwe ali ndi kachilombo ka HCV amakhala ndi ma antibodies mpaka ma proterict a NS5 osakhala makina a kachilomboka. Pachifukwa ichi, mayesowo akuphatikizapo ma antigens dera la NS5 ya gerul genome kuphatikiza pa NS3 (C200), NS4 (C2) ndi pachimake (C200).

Mfundo ya njirayi

Membrane wa chipangizo choyeserera ndi ophatikizidwa ndi HCV Antigen dera loyeserera ndipo Goat antibit igg igg igg dera. Pad pad ndi wokutidwa ndi fluorescence lotchedwa HCV Antigen ndi kalulu igg pasadakhale. Mukamayesa zitsanzo zabwino, antibody a hcv mu makanema ophatikizira ndi phyvscence yolembedwa hCV antigen, ndikupanga osakaniza. Mothandizidwa ndi immunoromatography, kulowerera kolowera m'mphepete mwa njira yoyesera, kuphatikizidwa ndi HCV ku Antigen kuphatikizidwa ndi ma fluorescence siginecha ya HCV Antibody mu zitsanzo zitha kupezeka ndi fluorescence immunosm

Zoyeserera ndi zida zoperekedwa

25T:
.Teadi khadiyo payokha yomwe yatulutsidwa ndi deta
.Satherps
.

Zida zofunika koma sizinaperekedwe
Chidebe chotolera, Timer
Zosunga Zitsanzo ndi Kusunga
1.Maso oyesedwa amatha kukhala seramu, heparin anticoagulant plasma kapena edta anticoagulant plasma.
2.Kugwirizana ndi maluso oyambira kutolera Sampu. Seramu kapena plasma zitsanzo zimatha kusungunuka mu 2-8 ℃ kwa 7s kwa 7s ndi cryphopulo ya cryprocy m'munsi - miyezi 6
3.All temple Pewani kuzungulira kwaming'alu.

Njira Yanjira
Chonde werengani buku la GAWO LA GAWO NDI Phukusi lisanachitike musanayesedwe.
. Zotsatira zoyeserera zangotchulidwa zamankhwala, siziyenera kukhala maziko okhawo omwe matenda azachipatala ndi chithandizo, mbiri yazachipatala, kupenda kwina, kupenda kwina, Epidemiology ndi zidziwitso zina .
. Sogonint imagwiritsidwa ntchito kokha kwa seramu ndi plasma. Mwina sizingapeze zotsatira zolondola mukamagwiritsa ntchito zitsanzo zina monga malovu ndi mkodzo ndi zina.

Makhalidwe Akugwirira Ntchito

Maumboni
1.Post kuthiridwa Hepatitis. Mu: Moore SB, Ed. Mankhwala osokoneza magazi. Amineton, VA. Ndili. Akuphatikiza. Mabanki a Magazi, mas. 53-38.
2.Anse jh, et al.hama kulowerera ndi Murine Moonlol antinossiays (j]
3.Levinson SS.uvinson ya mankhwala a heterophilic ndi udindo wa informsm [j].
4.Larter Hj., Purcell RH, Holland PV, et al. (1978) Wogwiritsa ntchito kunka kutanthauzira mosadetsedwa, osakhala a Bhepatitis. Lancet i: 459-463.
5.Claoo ql, wener Aj, rorby lr, oo g, horton m. (1990) HAPTIITIS C. (1990) Msuzi wamkulu wa viral osakhala, osakhala Bhepatitis. Br Med ng'ombe 46: 423-4441.
6.Engvall e, perlmann p. (1971) enzyme yolumikizidwa immunorbent metay (Elisa): lingaliro loyenerera la igg. Imfoshimtestry 8: 871-874.

Malingaliro Akuyembekezeka

HCV -B <0.02

Ndikulimbikitsidwa kuti labotale iliyonse ya labotale imayimira mtundu wake woyimira anthu ake.

Zotsatira ndi kutanthauzira

• Zomwe zili pamwambazi ndizotsatira za HCV-AB PRenter yovomerezeka, ndipo imanenedwa kuti labotale iliyonse iyenera kuwunika mitundu ya HCV-AM yomwe iyenera kupezeka kwa anthu kuderali. Zotsatira zomwe zili pamwambapa ndizongoyambira.
• Zotsatira za njirayi zimangogwira ntchito zomwe zimakhazikitsidwa mwanjira iyi, ndipo palibe kusiyana kwakukulu ndi njira zina.
• Zina zomwe zingapangitsenso zolakwitsa m'manyanda, kuphatikizapo zifukwa zaukadaulo, zolakwika zogwirira ntchito ndi zitsanzo zina.

Kusunga ndi kukhazikika

1. Kit ndi alumali miyezi 18 kuchokera tsiku lopanga. Sungani ma nduna osagwiritsidwa ntchito pa 2-30 ° C. Osazizira. Osagwiritsa ntchito kupitirira tsiku lomaliza.
2. Osatsegula thumba losindikizidwa mpaka mutakonzekera kuyesedwa, ndipo mayeso ogwiritsira ntchito amafunikira kugwiritsidwa ntchito pansi pa malo ofunikira (kutentha 2-35 ℃, chinyezi 40-9%) mwachangu .
3. Omwe amawagwiritsa ntchito atatsegulidwa atatsegulidwa.

Machenjezo ndi Zosamala
. Chipangizocho chikuyenera kusindikizidwa ndikutetezedwa ku chinyezi.
Malingaliro abwino adzavomerezedwa ndi njira zina.
.Malingaliro onse adzathandizidwa ndi zodetsa.
.Osagwiritsa ntchito zolimbitsa thupi.
.Osasokoneza zosintha pakati pa ma kits okhala ndi Mata ambiri ayi ..
.Osagwiritsanso ntchito makhadi oyesa komanso zowonjezera zilizonse zotayika.
.Zolakwika, mopitirira muyeso kapena zochepa zimatha kuyambitsa zopatuka.

LChifanizo
.Monga momwe mungagwiritsire ntchito ma antibodies ogwiritsa ntchito mbewa, kuthekera komwe kumachitika chifukwa cha ma antibodies a anti-mbewa (hama) m'chifanizo. Zofanana ndi odwala omwe alandila ma antibolodies a Monoclonies a matenda a matenda kapena chithandizo akhoza kukhala ndi hama. Zolinga zake zitha kuyambitsa zotsatira zabodza kapena zabodza.
Chinsinsi cha Zizindikiro Zogwiritsidwa Ntchito:

	Mu diagracic Diactic chipangizo chowonjezera
	Kupanga
	Sitolo pa 2-30 ℃
	Tsiku lothera ntchito
	Osagwiritsanso ntchito
	Chenjezo
	Konzani malangizo