Matenda a dianiac Trotonin I(fluorescence immunoromatophic stay)
Chifukwa mu diacro wozindikira okha
Chonde werengani phukusi ili musanagwiritsidwe ntchito ndikutsatira mwachikondi malangizowo. Kudalirika kwa zotsatira za Astay sikungatsimikizidwe ngati pali zopatuka pazinthu zomwe zili mu phukusi ili.

Kugwiritsa Ntchito
Diagnostic King Crotonin Issunon Immunochrophic Chimalowa. Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina. Kuyesedwa uku kumapangidwa kuti azigwiritsa ntchito zaumoyo wathanzi zokha.

Chidule
Miyezo ya CTNI idachulukana maola angapo pambuyo pa uthocardial infarction idachitika, idakwera pa 12-16 maola, ndipo adakhazikika patadutsa masiku 4-9 pambuyo pa myocardial infarction idachitika. Tanthauzo lapadziko lonse lapansi lachitatu lolowera mu 2012: Wokondedwa wa Biomeker-CTN (I kapena T), ali ndi minofu yayikulu ya myocardial ndi chidwi chachikulu. Zosintha mu kupsinjika kwa CTN ndikofunikira pakuzindikira kwa ami

Mfundo ya njirayi
Membrane wa chipangizo choyeserera ndi cholumikizidwa ndi anti ctni antibody padera loyeserera ndi Goat antibit IGG IGG Degred dera. Pad pad ndi wokutidwa ndi fluorescence yolembedwa anti ctni antibody ndi kalulu igg pasadakhale. Mukamayesa zitsanzo zabwino, CTNI antigen mu makanema ophatikizika ndi anzeru omwe a Anti Ctni Canibody, ndikupanga osakaniza. Mothandizidwa ndi immunoromatography, kulowerera kolowera m'mphepete mwa mayeso, pomwe panali dera loyesa, lomwe limaphatikizidwa ndi anti contraved antibody, ndi kuchuluka kwa ma fluorescence stre Mu zitsanzo zitha kupezeka ndi fluorescence immunossassisy.

Zoyeserera ndi zida zoperekedwa

25T:
Khadi la mayeso payokha kuti foil ofiirira ndi deta
Makanema apansi pa 25t
Phukusi

Zida zofunika koma sizinaperekedwe
Chidebe chotolera, Timer

Zosunga Zitsanzo ndi Kusunga
1.Maso oyesedwa amatha kukhala seramu, heparin anticoagulant plasma kapena edta anticoagulant plasma.
2.Kugwirizana ndi maluso oyambira kutolera Sampu. Seramu kapena plasma zitsanzo zimatha kusungunuka mu 2-8 ℃ kwa 7days ndi chgeroptar orsict -15 ° imayang'aniridwa pansipa -15 °
3.All temple Pewani kuzungulira kwaming'alu.

Njira Yanjira
Chonde werengani buku la GAWO LA GAWO NDI Phukusi lisanachitike musanayesedwe.
1.Tikweza ma reagents onse ndi zitsanzo kutentha.
2.Pen Worser Worlint (Wiz-A101), Lowetsani Chinsinsi cha Akaunti Malinga ndi Njira Yogwirira Ntchitoyo, ndikulowetsa mawonekedwe.
3.Scan nambala ya decotion kuti itsimikizire chinthucho.
4.Tukani khadi yoyeserera kuchokera m'thumba la foil.
5. Onani khadi yoyeserera mu khadi la khadi, jambulani nambala ya QR, ndikuwonetsa chinthucho.
6.dd 40μl seramu kapena plasma zitsanzo kuti zisangalatse, ndikusakaniza bwino ..
7.dd 80μl zitsanzo zothetsera chiwonetsero cha khadi.
8.CLLick "Medial Kuyesa", patatha mphindi 15, chida chimatha kudziwa khadi yoyeserera, imatha kuwerenga zotsatira kuchokera pazenera la chipangizocho, ndikulemba / kusindikiza zotsatira zake.
9.Lemiyer ku malangizo a wopenda wosambira (Wiz-A101).

Malingaliro Akuyembekezeka
Ctni <0.3ng / ml
Ndikulimbikitsidwa kuti labotale iliyonse ya labotale imayimira mtundu wake woyimira anthu ake.

Zotsatira ndi kutanthauzira
. Zotsatira zomwe zili pamwambapa ndizongoyambira.
. Zotsatira za njirayi zimangogwira ntchito zomwe zimakhazikitsidwa mwanjira iyi, ndipo palibe kusiyana pakati pa njira zina ndi njira zina.
.

Kusunga ndi kukhazikika
1.Tichisi ndi alumali miyezi 18 kuchokera tsiku lopanga. Sungani ma nduna osagwiritsidwa ntchito pa 2-30 ° C. Osazizira. Osagwiritsa ntchito kupitirira tsiku lomaliza.
2.Kosatsegula thumba losindikizidwa mpaka mutakonzekera kuyesedwa, ndipo mayeso ogwiritsira ntchito amapangidwira kugwiritsidwa ntchito pansi pa malo ofunikira (kutentha 2-35 ℃, chinyezi 40-9%) mkati mwa 60 mwachangu momwe mungathere.
3. Amagwirira ntchito amagwiritsidwa ntchito atatsegulidwa atatsegulidwa.

Machenjezo ndi Zosamala
. Chipangizocho chikuyenera kusindikizidwa ndikutetezedwa ku chinyezi.
Malingaliro abwino adzavomerezedwa ndi njira zina.
. Zoyesayesa zidzaperekedwa ngati zodetsa.
.Do osagwiritsa ntchito reagent.
.Do osasinthana pakati pa ma kits ndi Mata ambiri ayi ..
.Do osagwiritsanso ntchito makhadi oyesera komanso zowonjezera zilizonse zotayika.
.Misation, zitsanzo zochulukirapo kapena zochepa zimatha kuyambitsa zopatuka.

LChifanizo
. Zofanana ndi odwala omwe alandila ma antibolodies a Monoclonies a matenda a matenda kapena chithandizo akhoza kukhala ndi hama. Zolinga zake zitha kuyambitsa zotsatira zabodza kapena zabodza.
. Zotsatira zoyeserera zangotchulidwa zamankhwala, siziyenera kukhala maziko okhawo omwe matenda azachipatala ndi chithandizo, mbiri yazachipatala, kupenda kwina, kupenda kwina, Epidemiology ndi zidziwitso zina .
. Sogonint imagwiritsidwa ntchito kokha kwa seramu ndi plasma. Mwina sizingapeze zotsatira zolondola mukamagwiritsa ntchito zitsanzo zina monga malovu ndi mkodzo ndi zina.

Makhalidwe Akugwirira Ntchito

RZokonda

1.Tanse jh, et al.hama kulowerera kwa Murine Moonlol antinossiays (j]
2.levinson SS.uvinson ya ma antibodies a ma antibodies ndi gawo lomwe limagwirira ntchito (j] .j of fintumosm infwasm, 1992,15: 108-114.

Chinsinsi cha Zizindikiro Zogwiritsidwa Ntchito:

	Mu diagracic Diactic chipangizo chowonjezera
	Kupanga
	Sitolo pa 2-30 ℃
	Tsiku lothera ntchito
	Osagwiritsanso ntchito
	Chenjezo
	Konzani malangizo

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