Diagnostic Kit ya Alpha-fetoprotein(fluorescence immunochromatographic assay)
Kwa in vitro diagnostic ntchito kokha
Chonde werengani phukusili Ikani mosamala musanagwiritse ntchito ndikutsatira malangizowo. Kudalirika kwa zotsatira zoyesa sikungatsimikizidwe ngati pali zolakwika zilizonse kuchokera pamalangizo omwe ali mu phukusili.

ZOFUNIKA KUGWIRITSA NTCHITO

Diagnostic Kit for Alpha-fetoprotein (fluorescence immunochromatographic assay) ndi fluorescence immunochromatographic assay ya kuchuluka kwa Alpha-fetoprotein (AFP) mu seramu yamunthu kapena plasma, yomwe imagwiritsidwa ntchito makamaka pozindikira matenda owonjezera, machiritso komanso kuwonetsa kwa hepatocellular carcinoma. Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina. Kuyezetsa kumeneku kumangogwiritsidwa ntchito ndi akatswiri azachipatala okha.

CHIDULE

Alpha-fetoprotein (AFP) ndi imodzi mwa zolembera zomwe zimagwiritsidwa ntchito kwambiri. Ndi glycoprotein yokhala ndi molekyulu yolemera 70,000 ndi shuga 4%. Imapangidwa makamaka ndi chiwindi cha fetal, motsatiridwa ndi yolk sac. Masabata 6, kufika pachimake pa masabata 12 mpaka 15, kuchuluka kwa seramu mu 1 mpaka 3 g/L, ndi umbilical cord magazi pakubadwa kwa 10 mpaka 100 mg/L; 90 mpaka 500 ng/mL pakati;Zomwe zili mu seramu yamunthu ya AFP zimakhala pakati pa 2 ndi 8 ng/mL, koma matenda ambiri, makamaka a chiwindi, amakhudza mtengo wa AFP.

MFUNDO YA NJIRA

Nembanemba ya chipangizo choyesera imakutidwa ndi anti AFP antibody pagawo loyesa komanso anti-Rabbit IgG antibody pagawo lowongolera. Lable pad amakutidwa ndi fluorescence olembedwa anti AFP antibody ndi kalulu IgG pasadakhale. Mukayesa zitsanzo zabwino, antigen ya AFP mu zitsanzo imaphatikizana ndi fluorescence yotchedwa anti AFP antibody, ndikupanga kusakaniza kwa chitetezo chamthupi. Pansi pa zochita za immunochromatography, kuyenda movutikira molunjika ku pepala loyamwa, pamene zovuta zidadutsa gawo loyesa, zimaphatikizana ndi anti AFP zokutira antibody, zimapanga zovuta zatsopano. mu zitsanzo zitha kudziwika ndi fluorescence immunoassay assay.

REAGENTS NDI Zipangizo ZOPEREKA

Zigawo za phukusi la 25T:

.Khadi loyesera payokha zojambulazo zopakidwa ndi desiccant 25T
.Sample diluents 25T
.Phukusi loyika 1

ZINTHU ZOFUNIKA KOMA ZOSAPATSIDWA
Zitsanzo zosonkhanitsira chidebe, timer
KUSONKHA ZITSANZO NDI KUSINTHA
1.Zitsanzo zoyesedwa zingakhale seramu, heparin anticoagulant plasma kapena EDTA anticoagulant plasma.
2.Malinga ndi njira zoyenera sonkhanitsani zitsanzo. Seramu kapena madzi a m'magazi amatha kusungidwa mufiriji pa 2-8 ℃ kwa 7days ndi cryopreservation pansi -15 ° C kwa miyezi 6.
3.Zitsanzo zonse zimapewa kuzizira kozizira.

NJIRA YOYENERA
Chonde werengani buku lothandizira zida ndi kuyika phukusi musanayese.
1.Ikani pambali ma reagents onse ndi zitsanzo kutentha kutentha.
2.Tsegulani Portable Immune Analyzer (WIZ-A101), lowetsani mawu achinsinsi a akaunti malinga ndi njira yogwiritsira ntchito chida, ndikulowetsani mawonekedwe ozindikira.
3.Scan code dentification kuti mutsimikizire chinthu choyesera.
4.Tulutsani khadi loyesera muthumba la zojambulazo.
5.Lowetsani khadi loyesera mu kagawo ka khadi, jambulani kachidindo ka QR, ndi kuzindikira chinthu choyesera.
6.Add 20μL seramu kapena madzi a m'magazi zitsanzo diluent, ndi kusakaniza bwino.
7.Onjezani 80μL yachitsanzo chachitsulo kuti muyese bwino khadi.
8.Dinani batani la "standard test", pakatha mphindi 15, chidacho chidzangozindikira khadi yoyesera, imatha kuwerenga zotsatira kuchokera pachiwonetsero chowonetsera chida, ndikulemba / kusindikiza zotsatira zoyesa.
9. Onani malangizo a Portable Immune Analyzer(WIZ-A101).

MFUNDO ZOYENERA

AFP: ＜10ng/mL
Ndikoyenera kuti labotale iliyonse ikhazikitse mitundu yakeyake yomwe ikuyimira odwala ake.

ZOTSATIRA ZA MAYESE NDI KUMASULIRA
.Deta yomwe ili pamwambayi ndi zotsatira za mayeso a AFP reagent, ndipo akulangizidwa kuti labotale iliyonse iyenera kukhazikitsa mitundu yambiri ya AFP yodziwika bwino kwa anthu a m'derali. Zotsatira zomwe zili pamwambazi ndizongowona.
.Zotsatira za njirayi zimangogwiritsidwa ntchito pazigawo zowonetsera zomwe zakhazikitsidwa mu njira iyi, ndipo palibe kufananizidwa kwachindunji ndi njira zina.
.Zinthu zina zingayambitsenso zolakwika pazotsatira zodziwika, kuphatikizapo zifukwa zamakono, zolakwika zogwirira ntchito ndi zitsanzo zina.

KUSINTHA NDI KUKHALA
1. The zida ndi 18 miyezi alumali moyo kuyambira tsiku kupanga. Sungani zida zosagwiritsidwa ntchito pa 2-30 ° C. OSATI MASIMA. Osagwiritsa ntchito kupitirira tsiku lotha ntchito.
2.Musatsegule thumba losindikizidwa mpaka mutakonzeka kuyesa, ndipo kuyesa kamodzi kokha kukuyenera kugwiritsidwa ntchito pansi pa malo ofunikira (kutentha 2-35 ℃, chinyezi 40-90%) mkati mwa mphindi 60 mwamsanga. momwe zingathere.
3.Sample diluent imagwiritsidwa ntchito mwamsanga mutangotsegulidwa.

CHENJEZO NDI CHENJEZO
.Chidacho chiyenera kutsekedwa ndi kutetezedwa ku chinyezi.
.Zitsanzo zonse zabwino zidzatsimikiziridwa ndi njira zina.
.Zitsanzo zonse ziyenera kuonedwa ngati zowononga.
.MUSAMAGWIRITSE NTCHITO zomwe zidatha.
. OSATI osinthana ma reagents pakati pa zida zokhala ndi magawo osiyanasiyana No.
.MUSAMAGWIRITSE NTCHITO makhadi oyesera ndi zina zilizonse zomwe zingathe kutayidwa.
.Misoperation, mochulukira kapena pang'ono zitsanzo kungayambitse zotsatira zopotoka.

LKUTSANZITSA
.Monga ndi kuyesa kulikonse komwe kumagwiritsa ntchito ma antibodies a mbewa, kuthekera kulipo kuti kusokonezedwe ndi ma antibodies a anti-mbewa (HAMA) pachitsanzo. Zitsanzo za odwala omwe alandira kukonzekera kwa ma antibodies a monoclonal kuti adziwe matenda kapena mankhwala angakhale ndi HAMA. Zitsanzo zoterezi zingayambitse zotsatira zabodza kapena zabodza.
.Chotsatira choyezetsa ichi ndi chachipatala chokha, sichiyenera kukhala maziko okhawo a matenda ndi chithandizo chamankhwala, chithandizo chamankhwala cha odwala chiyenera kuganiziridwa mozama pamodzi ndi zizindikiro zake, mbiri yachipatala, kufufuza kwa labotale, kuyankha kwamankhwala, miliri ndi zina. .
.Reagent iyi imagwiritsidwa ntchito poyezetsa seramu ndi plasma. Ikhoza kusapeza zotsatira zolondola zikagwiritsidwa ntchito pa zitsanzo zina monga malovu ndi mkodzo ndi zina.

ZINTHU ZOCHITIKA

RMALANGIZO
1.Hansen JH, ndi al.HAMA Kusokoneza ndi Murine Monoclonal Antibody-Based Immunoassays [J].J ya Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Chilengedwe cha Heterophilic Antibodies ndi Udindo mu Immunoassay Interference[J].J wa Clin Immunoassay,1992,15:108-114.

Chinsinsi cha zizindikiro zogwiritsidwa ntchito:

	In Vitro Diagnostic Medical Chipangizo
	Wopanga
	Sungani pa 2-30 ℃
	Tsiku lothera ntchito
	Osagwiritsanso Ntchito
	CHENJEZO
	Funsani Malangizo Ogwiritsa Ntchito

Xiamen Wiz Biotech CO., LTD
Address:3-4 Floor,NO.16 Building,Bio-medical Workshop,2030 Wengjiao West Road,Haicang District,361026,Xiamen,China
Tel: + 86-592-6808278
Fax: + 86-592-6808279