Diagnostic Kit ya Procalcitonin

(fluorescence immunochromatographic assay)

Kwa in vitro diagnostic ntchito kokha

Chonde werengani phukusili Ikani mosamala musanagwiritse ntchito ndikutsatira malangizowo. Kudalirika kwa zotsatira zoyesa sikungatsimikizidwe ngati pali zolakwika zilizonse kuchokera pamalangizo omwe ali mu phukusili.

ZOFUNIKA KUGWIRITSA NTCHITO

Diagnostic Kit for Procalcitonin (fluorescence immunochromatographic assay) ndi fluorescence immunochromatographic assay pakuzindikira kuchuluka kwa Procalcitonin (PCT) mu seramu yamunthu kapena plasma, imagwiritsidwa ntchito pozindikira matenda oyambitsidwa ndi bakiteriya ndi sepsis. Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina. Kuyezetsa kumeneku kumangogwiritsidwa ntchito ndi akatswiri azachipatala okha.

CHIDULE

Procalcitonin imapangidwa ndi 116 amino acid ndipo kulemera kwake ndi 12.7KD. PCT imawonetsedwa ndi ma cell a neuroendocrine ndikuphwanyidwa ndi michere kukhala (immature) calcitonin, carboxy-terminating peptide, ndi amino terminting peptide. Anthu omwe ali ndi thanzi labwino amakhala ndi PCT yochepa m'magazi awo, omwe amatha kuwonjezeka kwambiri pambuyo pa matenda a bakiteriya. Pamene sepsis imapezeka m'thupi, minyewa yambiri imatha kufotokoza PCT, kotero PCT ikhoza kugwiritsidwa ntchito ngati chizindikiro cha sepsis. Kwa odwala ena omwe ali ndi matenda otupa, PCT ingagwiritsidwe ntchito ngati chizindikiro cha kusankha maantibayotiki ndi chiweruzo chothandiza.

MFUNDO YA NJIRA

Nembanemba ya chipangizo choyesera imakutidwa ndi anti PCT antibody pagawo loyesa komanso anti-Rabbit IgG antibody pagawo lowongolera. Lable pad amakutidwa ndi fluorescence olembedwa anti PCT antibody ndi kalulu IgG pasadakhale. Mukayesa zitsanzo zabwino, antigen ya PCT mu zitsanzo imaphatikizana ndi fluorescence yotchedwa anti PCT antibody, ndikupanga kusakaniza kwa chitetezo cha mthupi. Pansi pa zochita za immunochromatography, kuyenda movutikira kumayendedwe a pepala loyamwa, pamene zovuta zidadutsa dera loyesa, kuphatikiza ndi anti PCT ❖ kuyanika antibody, zimapanga zovuta zatsopano. Mulingo wa PCT umalumikizidwa bwino ndi siginecha ya fluorescence, ndipo kuchuluka kwa PCT mu zitsanzo kumatha kudziwika ndi fluorescence immunoassay assay.