MFUNDO

Nembanemba ya chipangizo choyesera imakutidwa ndi antibody ya Cpn-IgM pagawo loyeserera ndi anti-Rabbit IgG antibody pagawo lowongolera. Lable pad amakutidwa ndi fluorescence otchedwa anti Cpn-IgM antibody ndi kalulu IgG pasadakhale. Mukayesa zitsanzo zabwino, antigen ya Cpn-IgM mu zitsanzo imaphatikiza ndi fluorescence yotchedwa anti Cpn-IgM antibody, ndikupanga kusakaniza kwa chitetezo cha mthupi. Pansi pa zochita za immunochromatography, zovuta kuyenda mu malangizo a absorbent pepala. Pamene zovuta zidadutsa gawo loyesa, zimaphatikizidwa ndi anti-Cpn-IgM zokutira antibody, zimapanga zovuta zatsopano. Mzere wofiyira ndi muyezo womwe umawonekera m'dera lowongolera (C) kuti muwone ngati pali zitsanzo zokwanira komanso ngati njira ya chromatography ndi yabwinobwino. Amagwiritsidwanso ntchito ngati muyezo wowongolera mkati mwa ma reagents.

Njira Yoyesera

Njira yoyesera ya WIZ-A202 ndi Portable Immune analyzer WIZ-A101 imatanthawuza malangizo a malangizo. Njira yoyeserera yowonera ili motere:

1. Tulutsani khadi loyesera mu thumba la zojambulazo, liyikeni pa tebulo lapamwamba ndikuyika chizindikiro.
2. Onjezani 10μl seramu kapena madzi a m'magazi kapena 20ul wathunthu wamagazi kuti muyese bwino pakhadi ndi dispette yoperekedwa, kenaka yikani 100μl (pafupifupi 2-3 dontho) chitsanzo chochepetsera; kuyamba nthawi.
3. Dikirani kwa mphindi zosachepera 10-15 ndikuwerenga zotsatira zake, zotsatira zake ndizosavomerezeka pakatha mphindi 15.