MFUNDO

Nembanemba ya chipangizo choyesera imakutidwa ndi antibody ya Tf pagawo loyeserera ndi anti-Rabbit IgG antibody pagawo lowongolera. Padi lable amakutidwa ndi fluorescence olembedwa anti Tf antibody ndi kalulu IgG pasadakhale. Mukayesa zitsanzo zabwino, antigen ya Tf mu zitsanzo imaphatikizana ndi fluorescence yotchedwa anti Tf antibody, ndikupanga kusakaniza kwa chitetezo chamthupi. Pansi pa zochita za immunochromatography, zovuta kuyenda mu malangizo a absorbent pepala. Pamene zovuta zidadutsa gawo loyesa, zimaphatikizidwa ndi anti Tf coating antibody, zimapanga zovuta zatsopano.

Ngati zili zoipa, chitsanzocho chilibe transferrin kapena zomwe zili ndi zochepa, kotero kuti chitetezo cha mthupi sichingapangidwe. Sipadzakhala mzere wofiira pamalo ozindikira (T). Ziribe kanthu kuti antibody ya Tf ilipo kapena ayi, mbewa yotsalira ya golide yokhala ndi zilembo zotsutsana ndi munthu IgM ndi anti-mbewa IgG antibody yomwe idakutidwa pamzere wowongolera (C). Kenako ma agglutinates amapanga mtundu m'dera lowongolera, ndipo mzere wofiira udzawonekera mu (C). Mzere wofiyira ndi muyezo womwe umawonekera m'dera lowongolera (C) kuti muwone ngati pali zitsanzo zokwanira komanso ngati njira ya chromatography ndi yabwinobwino. Amagwiritsidwanso ntchito ngati muyezo wowongolera mkati mwa ma reagents.

Njira Yoyesera

Chonde werengani phukusili musanayese.

1.Tulutsani khadi loyesera kuchokera mu thumba la zojambulazo, liyikeni pa tebulo lapamwamba ndikuyika chizindikiro.

2.Chotsani kapu kuchokera ku chubu lachitsanzo ndikutaya madontho awiri oyambirira osungunuka, onjezerani madontho a 3 (pafupifupi 100uL) palibe kuwira kuchepetsedwa chitsanzo vertically ndi pang'onopang'ono mu chitsanzo chitsime cha khadi ndi dispette anapereka. Kenako yambani chowerengera.