MFUNDO

Nembanemba ya chipangizo choyesera imakutidwa ndi antigen ya Rotavirus Gulu A pagawo loyeserera ndi anti-anti rabbit IgG antibody pachigawo chowongolera. Lable pad amakutidwa ndi fluorescence olembedwa anti Rotavirus Gulu A ndi kalulu IgG pasadakhale. Mukayesa zitsanzo zabwino, RV mu zitsanzo imaphatikizana ndi fluorescence yolembedwa kuti anti Rotavirus Gulu A, ndikupanga kusakaniza kwa chitetezo chamthupi. Pansi pa zochita za immunochromatography, zovuta kuyenda mu malangizo a absorbent pepala. Pamene zovuta zidadutsa gawo loyesa, zimaphatikizana ndi anti-Rotavirus Gulu A zokutira antibody, zimapanga zovuta zatsopano. Ngati zili zoipa, palibe antigen ya Rotavirus Gulu A mu chitsanzo, kotero kuti zovuta za chitetezo cha mthupi zisapangidwe, sipadzakhala mzere wofiira pamalo ozindikira (T). Kaya gulu A rotavirus alipo mu chitsanzo, mbewa zolembedwa latex IgG ndi chromatographed kumalo olamulira khalidwe (C) ndi kugwidwa ndi mbuzi anti-mbewa IgG antibody. Mzere wofiira udzawonekera mu malo olamulira khalidwe (C). Mzere wofiyira ndi muyezo womwe umawonekera m'dera lowongolera (C) kuti muwone ngati pali zitsanzo zokwanira komanso ngati njira ya chromatography ndi yabwinobwino. Amagwiritsidwanso ntchito ngati muyezo wowongolera mkati mwa ma reagents.

Njira Yoyesera:

1.Odwala ozindikira ayenera kusonkhanitsidwa. Malinga ndi malipoti, excretion pazipita rotavirus mu ndowe za odwala gastroenteritis kumachitika 3-5 patatha masiku chiyambi cha matenda ndi 3-13 masiku chiyambi cha zizindikiro. Ngati chitsanzo chasonkhanitsidwa nthawi yayitali pambuyo pa kutsekula m'mimba, chiwerengero cha ma antigen sichingakhale chokwanira kuti chichitike.

2.Zitsanzozi ziyenera kusonkhanitsidwa mu chidebe choyera, chouma, chopanda madzi chomwe mulibe zotsukira ndi zotetezera.

3.Kwa odwala osatsegula m'mimba, zitsanzo za ndowe zomwe zasonkhanitsidwa zisakhale zosachepera 1-2 magalamu. Kwa odwala matenda otsekula m'mimba, ngati ndowe ndi madzi, chonde tengani 1-2 ml ya ndowe zamadzimadzi. Ngati ndowe zili ndi magazi ambiri ndi mamina, chonde sonkhanitsaninso nyembazo.

4.Tikulimbikitsidwa kuyesa zitsanzo mwamsanga mutatha kusonkhanitsa, mwinamwake ziyenera kutumizidwa ku labotale mkati mwa maola 6 ndikusungidwa pa 2-8 ° C. Ngati zitsanzozo sizinayesedwe mkati mwa maola 72, ziyenera kusungidwa pa kutentha kosachepera -15 ° C.

5. Gwiritsani ntchito ndowe zatsopano poyesa, ndi zitsanzo za ndowe zosakanikirana ndi madzi osungunuka kapena osungunuka