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Kwa in vitro diagnostic ntchito kokha

Chonde werengani phukusili Ikani mosamala musanagwiritse ntchito ndikutsatira malangizowo. Kudalirika kwa zotsatira zoyesa sikungatsimikizidwe ngati pali zolakwika zilizonse kuchokera pamalangizo omwe ali mu phukusili.

ZOFUNIKA KUGWIRITSA NTCHITO

Diagnostic Kit for Hepatitis C Virus Antibody (Fluorescence Immunochromatographic Assay) ndi fluorescence immunochromatographic assay pofuna kudziwa kuchuluka kwa ma antibody a HCV mu seramu yamunthu kapena plasma, yomwe ndi yofunikira pakuwunika kothandizira kwa matenda a chiwindi C. Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi ena njira. Kuyezetsa kumeneku kumangogwiritsidwa ntchito ndi akatswiri azachipatala okha

1.Ikani pambali ma reagents onse ndi zitsanzo kutentha kutentha.
2.Tsegulani Portable Immune Analyzer (WIZ-A101), lowetsani mawu achinsinsi a akaunti malinga ndi njira yogwiritsira ntchito chida, ndikulowetsani mawonekedwe ozindikira.
3.Scan code dentification kuti mutsimikizire chinthu choyesera.
4.Tulutsani khadi loyesera muthumba la zojambulazo.
5.Lowetsani khadi loyesera mu kagawo ka khadi, jambulani kachidindo ka QR, ndi kuzindikira chinthu choyesera.
6.Add 20μL seramu kapena madzi a m'magazi zitsanzo diluent, ndi kusakaniza bwino.
7.Onjezani 80μL yachitsanzo chachitsulo kuti muyese bwino khadi.
8.Dinani batani la "standard test", pakatha mphindi 15, chidacho chidzangozindikira khadi yoyesera, imatha kuwerenga zotsatira kuchokera pachiwonetsero chowonetsera chida, ndikulemba / kusindikiza zotsatira zoyesa.
9. Onani malangizo a Portable Immune Analyzer(WIZ-A101).

CHIDULE

Kachilombo ka Hepatitis C (HCV) ndi kachirombo ka HIV kamene kamakhala ndi kachilombo ka RNA (9.5 kb) komwe kamachokera ku banja la Flaviviridae. Ma genotypes akuluakulu asanu ndi limodzi ndi magulu ang'onoang'ono a HCV adziwika. Atalekanitsidwa mu 1989, HCV tsopano yazindikirika monga choyambitsa chachikulu cha kuikidwa magazi kogwirizana ndi matenda a chiwindi a non-A, non-B. Matendawa amakhala ndi pachimake ndi aakulu mawonekedwe. Oposa 50 peresenti ya anthu omwe ali ndi kachilomboka amakhala ndi matenda a chiwindi oopsa kwambiri, omwe amawopsyeza moyo ndi matenda a chiwindi ndi hepatocellular carcinomas. Chiyambireni mu 1990 njira yoyezera magazi oletsa kudwala HCV, kuchuluka kwa matendawa kwa olandira magazi kwachepetsedwa kwambiri. Kafukufuku wachipatala amasonyeza kuti chiwerengero chachikulu cha anthu omwe ali ndi kachilombo ka HCV amapanga ma antibodies ku NS5 omwe si opangidwa ndi mapuloteni a kachilomboka. Pa izi, mayesowa amaphatikiza ma antigen ochokera kudera la NS5 la ma virus genome kuphatikiza NS3 (c200), NS4 (c200) ndi Core (c22).

MFUNDO YA NJIRA

Nembanemba ya chipangizo choyesera imakutidwa ndi antigen ya HCV pagawo loyesa ndi anti-Rabbit IgG antibody pagawo lowongolera. Lable pad amakutidwa ndi fluorescence otchedwa HCV antigen ndi kalulu IgG pasadakhale. Mukayesa zitsanzo zabwino, antibody ya HCV mu zitsanzo imaphatikizana ndi fluorescence yotchedwa HCV antigen, ndikupanga kusakaniza kwa chitetezo chamthupi. Pansi pa zochita za immunochromatography, otaya zovuta mu malangizo a kuyamwa pepala, pamene zovuta anadutsa mayeso dera, izo pamodzi ndi HCV Antigen ❖ kuyanika antigen, kupanga latsopano complex.HCV antibody mlingo ndi zabwino zogwirizana ndi fluorescence chizindikiro, ndi ndende ya Ma antibodies a HCV mu zitsanzo amatha kudziwika ndi fluorescence immunoassay assay

REAGENTS NDI Zipangizo ZOPEREKA

Zigawo za phukusi la 25T:
.Khadi loyesera payokha zojambulazo zopakidwa ndi desiccant
.Zitsanzo za diluent
.Kuyika phukusi

ZINTHU ZOFUNIKA KOMA ZOSAPATSIDWA
Zitsanzo zosonkhanitsira chidebe, timer
KUSONKHA ZITSANZO NDI KUSINTHA
1.Zitsanzo zoyesedwa zingakhale seramu, heparin anticoagulant plasma kapena EDTA anticoagulant plasma.
2.Malinga ndi njira zoyenera sonkhanitsani zitsanzo. Seramu kapena madzi a m'magazi amatha kusungidwa mufiriji pa 2-8 ℃ kwa 7days ndi cryopreservation pansipa -15 ° C kwa miyezi 6.
3.Zitsanzo zonse zimapewa kuzizira kozizira.

NJIRA YOYENERA
Chonde werengani buku lothandizira zida ndi kuyika phukusi musanayese.
.Chotsatira choyezetsa ichi ndi chachipatala chokha, sichiyenera kukhala maziko okhawo a matenda ndi chithandizo chamankhwala, chithandizo chamankhwala cha odwala chiyenera kuganiziridwa mozama pamodzi ndi zizindikiro zake, mbiri yachipatala, kufufuza kwa labotale, kuyankha kwamankhwala, miliri ndi zina. .
.Reagent iyi imagwiritsidwa ntchito poyezetsa seramu ndi plasma. Ikhoza kusapeza zotsatira zolondola zikagwiritsidwa ntchito pa zitsanzo zina monga malovu ndi mkodzo ndi zina.

ZINTHU ZOCHITIKA

MALONJE
1.Post transfusion hepatitis. Mu: Moore SB, ed. Matenda Opatsirana Opatsirana Opatsirana. Alington, PA. Ndi. Assoc. Mabanki a Magazi, tsamba 53-38.
2.Hansen JH, ndi al.HAMA Kusokoneza ndi Murine Monoclonal Antibody-Based Immunoassays [J].J ya Clin Immunoassay,1993,16:294-299.
3.Levinson SS.Chikhalidwe cha Heterophilic Antibodies ndi Udindo mu Immunoassay Interference[J].J wa Clin Immunoassay,1992,15:108-114.
4.Alter HJ., Purcell RH, Holland PV, et al. (1978) Wothandizira wopatsirana mu non-A, non-B hepatitis. Lancet I: 459-463.
5.Choo QL,Weiner AJ, Overby LR, Kuo G, Houghton M. (1990) Hepatitis C Virus: choyambitsa chachikulu cha tizilombo toyambitsa matenda omwe si A, omwe si a B. Br Med Bull 46: 423-441.
6.Engvall E, Perlmann P. (1971) Enzyme yogwirizana ndi immunosorbent assay (ELISA): khalidwe lapamwamba la IgG. Immunochemistry 8: 871-874.

MFUNDO ZOYENERA

HCV-Ab <0.02

Ndikoyenera kuti labotale iliyonse ikhazikitse mitundu yakeyake yomwe ikuyimira odwala ake.

ZOTSATIRA ZA MAYESE NDI KUMASULIRA

• Zomwe zili pamwambazi ndi zotsatira za kuyesa kwa HCV-Ab reagent, ndipo akuti labotale iliyonse iyenera kukhazikitsa mitundu ingapo yodziwikiratu ya HCV-Ab yoyenera anthu amderali. Zotsatira zomwe zili pamwambazi ndizongowona.
• Zotsatira za njirayi zimangogwira ntchito pazigawo zowonetsera zomwe zakhazikitsidwa mu njira iyi, ndipo palibe kufananizidwa kwachindunji ndi njira zina.
• Zinthu zina zingayambitsenso zolakwika pazotsatira zozindikiridwa, kuphatikiza zifukwa zaukadaulo, zolakwika zogwirira ntchito ndi zitsanzo zina.

KUSINTHA NDI KUKHALA

1. Zidazi ndi miyezi 18 ya alumali kuyambira tsiku lopangidwa. Sungani zida zosagwiritsidwa ntchito pa 2-30 ° C. OSATI MASIMA. Musagwiritse ntchito kupitirira tsiku lotha ntchito.
2. Osatsegula thumba losindikizidwa mpaka mutakonzeka kuyesa, ndipo mayeso ogwiritsira ntchito kamodzi akuyenera kugwiritsidwa ntchito pamalo ofunikira (kutentha 2-35 ℃, chinyezi 40-90%) mkati mwa mphindi 60 mwachangu momwe mungathere. .
3. Sample diluent amagwiritsidwa ntchito atangotsegula.

CHENJEZO NDI CHENJEZO
.Chidacho chiyenera kutsekedwa ndi kutetezedwa ku chinyezi.
.Zitsanzo zonse zabwino zidzatsimikiziridwa ndi njira zina.
.Zitsanzo zonse ziyenera kutengedwa ngati zowononga.
.OSATI kugwiritsa ntchito reagent yomwe yatha.
.OSATI kusinthanitsa ma reagents pakati pa zida zokhala ndi magawo osiyanasiyana No.
.OSAGWIRITSA NTCHITO makhadi oyesera ndi zida zilizonse zotayidwa.
.Kusagwira ntchito molakwika, kuchulukirachulukira kapena kuchepera pang'ono kumatha kubweretsa zotsatira zoyipa.

LKUTSANZITSA
.Mofanana ndi kuyesa kulikonse komwe kumagwiritsa ntchito ma antibodies a mbewa, kuthekera kulipo kosokoneza ma antibodies a anti-mbewa (HAMA) pachitsanzocho. Zitsanzo za odwala omwe alandira kukonzekera kwa ma antibodies a monoclonal kuti adziwe matenda kapena mankhwala angakhale ndi HAMA. Zitsanzo zoterezi zingayambitse zotsatira zabodza kapena zabodza.
Chinsinsi cha zizindikiro zogwiritsidwa ntchito:

	In Vitro Diagnostic Medical Chipangizo
	Wopanga
	Sungani pa 2-30 ℃
	Tsiku lothera ntchito
	Osagwiritsanso Ntchito
	CHENJEZO
	Funsani Malangizo Ogwiritsa Ntchito