SUMMARY

Vitamin D is a vitamin and is also a steroid hormone, mainly including VD2 and VD3, whose struction is very similar. Vitamin D3 and D2 are converted to 25 hydroxyl vitamin D(including 25-dihydroxyl vitamin D3 and D2). 25-(OH) VD in the human body, stable struction, high concentration. 25-(OH) VD reflects the total amount of vitamin D , and the conversion ability of vitamin D, so 25-(OH)VD is considered to be the best indicator for evaluating the level of vitamin D.The Diagnostic Kit is based on immunochromatography and can give a result within 15 minutes.

PRINCIPLE OF THE PROCEDURE

The membrane of the test device is coated with the conjugate of BSA and 25-(OH)VD on the test region and goat anti rabbit IgG antibody on the control region. Marker pad are coated by fluorescence mark anti 25-(OH)VD antibody and rabbit IgG in advance. When testing sample, 25-(OH)VD in sample combine with fluorescence marked anti 25-(OH)VD antibody, and form immune mixture. Under the action of the immunochromatography, the complex flow in the direction of absorbent paper, when complex passed the test region, The free fluorescent marker will be combined with 25-(OH)VD on the membrane.The concentration of 25-(OH)VD is negative correlation for fluorescence signal, and the concentration of 25-(OH)VD in sample can be detected by fluorescence immunoassay assay.