MĀTĀPONO

He paninga anti-cal McAb kei runga i te rohe whakamatautau me te koati anti-rabbit IgG antibody i runga i te rohe whakahaere, he mea mau ki te chromatography membrane i mua. Ko te papa tapanga he mea pani ki te koura colloidal kua tapaina ki te anti cal McAb me te colloidal koura kua tapaina ki te rapeti IgG antibody i mua. I te wa e whakamatautau ana i te tauira pai, ka uru mai te cal i roto i te tauira ki te koura colloidal kua tapaina he anti cal McAb, ka hanga i te matatini aukati, na te mea ka taea te heke i te taha o te tarai whakamatautau, ka mau te cal conjugate complex e te paninga anti cal McAb ki te kiriuhi me te ahua. Ko te “anti cal coating McAb-cal-colloidal gold labeled anti cal McAb” matatini, i puta he roopu whakamatautau tae ki te rohe whakamatautau. Ko te kaha o te tae he pai te hono ki te ihirangi cal. Ko te tauira toraro karekau e whakaputa i te roopu whakamatautau na te kore o te cal complex conjugate koura colloidal. Ahakoa he tauira kei roto i te tauira kaore ranei, ka kitea he karaehe whero ki te rohe tohutoro me te rohe whakahaere kounga, e kiia ana he paerewa hinonga o-roto kounga.

Tikanga Whakamatau:

1.Tangohia te rakau tauira, ka kuhu ki roto i te tauira tai, katahi ka whakahokia te rakau tauira, ka wiriwiri kia pai te wiri, ka tuaruatia te mahi kia toru nga wa. Ma te whakamahi ranei i te rakau tauira i kohia mo te 50mg tauira taima, ka maka ki roto i te ngongo tauira taima kei roto te waimeha tauira, ka wiriwiri kia piri.

2. Whakamahia te tauira paipa tuku tango i te tauira taima angiangi mai i te turoro matere, katahi ka tapiri kia 3 nga pata (pee ki te 100uL) ki te ngongo tauira taie ka wiri pai, waiho ki te taha.

3.Tangohia te kaari whakamatautau mai i te putea pepa, tuu ki runga i te teepu taumata ka tohu.

4.Tangohia te potae mai i te ngongo tauira ka maka i nga pata tuatahi e rua kua diluted tauira, tāpiri 4.drops (mo 100uL) kore mirumiru diluted tauira poutū, me te āta ki te tauira pai o te kāri me te dispette whakaratohia, tīmata wā.

5.Me panui te hua i roto i nga meneti 10-15, a ka muhu i muri i nga meneti 15